Fig. 5
Conditional knockout (cKO) of Yap1 in the stroma promotes in vivo tumor dissemination (A) The images (left) and quantifications (right) of the bioluminescence signals among Yap1+/+, Yap1+/− and Yap1−/−mice upon intraperitoneal injection of GFP/Luc-labelled ID8 cells from Day 7 to Day 49 (n = 3, mean ± SEM, 2-way ANOVA, **P < 0.01, ***P < 0.001). (B) Dot plots displayed the ascites volume among Yap1+/+, Yap1+/−, and Yap1−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 cells (n = 5, mean ± SEM). (C) Dot plots displayed the number of tumor nodules among Yap1+/+, Yap1+/−, and Yap1−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 cells (n = 5, mean ± SEM, t-test, **P < 0.01, ****P < 0.0001). (D) ELISA revealed the amount of GROα in the ascites of Yap1+/+, Yap1+/− and Yap1−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 cells (n = 3, mean ± SEM, t-test, **P < 0.01, ****P < 0.0001). (E) The images (left and right) and quantifications (middle) of the intraperitoneal epifluorescence signals among Yap1+/+, Yap1+/− and Yap1−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 cells (n = 3, mean ± SEM, t-test, * P < 0.05, **P < 0.01)