Fig. 6

Depletion of CXCR1/2 impairs the oncogenic potential of OvCa cells (A) XTT cell proliferation assay indicated the relative cell growth of ID8 and ID8 Cxcr1/2 CRISPRi cell lines when co-cultured with murine stromal conditioned medium from Yap1 stromal-specific cKO mice (Yap1^+/−-CM and Yap1^−/−-CM). Yap1^+/+-CM was used as a control (n = 6, mean ± SEM, 2-way ANOVA, ****P < 0.0001). N = 3 independent experiments. (B) The schematic diagram showed the workflow of how ID8 cells with Cxcr1/2 were silenced using CRISPR interference (CRISPRi) approach (ID8 Cxcr1/2 CRISPRi cells) were generated and intraperitoneally injected into Yap1^+/+, Yap1^+/− and Yap1^−/− mice to establish a mouse tumor model. (C) The images (left) and quantifications (right) of the bioluminescence signals among Yap1^+/+, Yap1^+/− and Yap1^−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 Cxcr1/2 CRISPRi cells from Day 7 to Day 49 (n = 3, mean ± SEM). (D) The images (left and right) and quantifications (middle) of the intraperitoneal epifluorescence signals among Yap1^+/+, Yap1^+/− and Yap1^−/− mice with an intraperitoneal injection of GFP/Luc-labelled ID8 Cxcr1/2 CRISPRi cells (n = 3, mean ± SEM)