Fig. 2
YTHDC2 regulates ZNRD1-AS1 via m6A modification. Correlation analysis between the expression of ZNRD1-AS1 and (A) METTL14 and (B) METTL3 in lung cancer datasets in TCGA database. Five sites with more than moderate confidence were verified by (C-D) meRIP-PCR and (E–F) meRIP qPCR in S30 and H1299 cells, the anti-IgG antibody was used as control. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs IgG control group. qPCR was performed to examine the relative expression levels of ZNRD1-AS1 in (G) S30 and (H) H1299 cells treated with 3-DAA (a global methylation inhibitor) or MA (an FTO inhibitor), for which GAPDH was used as an internal reference. ** P < 0.01 and *** P < 0.001 vs. DMSO group. qPCR was performed to detect the relative expression levels of ZNRD1-AS1 in 3-DAA-treated YTHDC2 overexpressing (I) S30 and (J) H1299 cells, ** P < 0.01 vs. DMSO blank group, # P < 0.05 and ## P < 0.01 vs. DMSO pYTHDC2 group. TCGA: The Cancer Genome Atlas. qPCR: quantitative PCR. meRIP: methylated RNA immunoprecipitation