Fig. 6
miR-942 is a downstream target of ZNRD1-AS1. A FISH was used to analyze the subcellular localization of ZNRD1-AS1 in S30 and H1299 cells. B RT-qPCR results of ZNRD1-AS1 expression in the cytoplasm and nucleus of S30 and H1299 cells. C Scatter plot showing the screening of ZNRD1-AS1 downstream target miRNAs using the lncBase online tool and correlation analysis. D miR-942 expression in CS-exposed cells and H1299 cell. ** P < 0.01 vs. BEAS-2B cells. E miR-942 expression levels in S30 cells with overexpression or knockdown of ZNRD1-AS1. F Correlation analysis of miR-942 and ZNRD1-AS1 in the TCGA lung cancer dataset. ** P < 0.01 vs. BEAS-2B group. G Expression of miR-942 in subcutaneous tumors formed by ZNRD1-AS1-overexpressing H1299 cells injected into nude mice. ** P < 0.01 vs. pCDH blank or pGreen blank. H Expression of miR-942 in tumor and normal tissues in TCGA lung cancer dataset. I The EdU proliferation assay was performed to analyze the proliferation ability of S30 cells following co-transfection of miR-942 and ZNRD1-AS1 in S30 cells. J The Transwell cell migration assay was performed to analyze the altered migration ability of S30 cells following co-transfection of miR-942 and ZNRD1-AS1. Different letters (a, b, c and d) represent statistically significant group differences (P < 0.05). K The binding sites of ZNRD1-AS1 and miR-942 were predicted using lncBase. (50) The dual-luciferase reporter assay was performed to determine the binding sites of ZNRD1-AS1 and miR-942. TCGA: The Cancer Genome Atlas. CS: cigarette smoke