Fig. 7
TNS1 is a ceRNA of ZNRD1-AS1. A A Venn diagram presents the intersection of miR-942 target genes predicted using four miRNA target gene prediction databases, including miRDB, miRTarBase, TargetScan and miRWalk, as well as the mRNAs correlated with miR-942 expression. Correlation analysis indicated the association between miR-942 and (B) TNS1 or (C) ZNRD1-AS1 in TCGA lung cancer datasets. Western blotting was performed to determine TNS1 protein expression levels in (D) miR-942 overexpressed S30 cells and (E) ZNRD1-AS1 overexpressed or knocked-down S30 cells. F IHC analysis of TNS1 protein expression levels in subcutaneous tumors formed from ZNRD1-AS1-overexpressed H1299 cells injected into nude mice. G The EdU proliferation assay was performed to analyze the proliferation ability of S30 cells following co-transfection of shTNS1 and pZNRD1-AS1 in S30 cells. H Transwell cell migration assay analysis of the altered migration ability after co-transfection of shTNS1 and pZNRD1-AS1 in S30 cells. The different letters (a, b, c and d) represent statistically significant group differences (P < 0.05). I The binding sites between the TNS1 3’UTR and miR-942 were predicted using the TargetScan online tool. J The dual-luciferase reporter assay was performed to determine the interaction between TNS1 and miR-942. ceRNAs: Competing endogenous RNAs. IHC: immunohistochemical. UTR: untranslated region