Fig. 2
From: Safety and antitumor activity of GD2-Specific 4SCAR-T cells in patients with glioblastoma
Design and killing assay of GD2 specific 4SCAR-T cells. A Design of GD2 specific 4SCAR-T cells. B Study design of killing assay of peripheral blood mononuclear cells (PBMCs), CD19 specific 4SCAR-T cells and GD2 specific 4SCAR-T cells against primary GBM cells. The primary GBM cells transduced with a lentiviral wasabi green fluorescence reporter gene were used as target cells, and plated into 48-well plate before co-culturing with different cells (E:T ratio 4:1). C Fluorescence microscopy of primary GBM cells from Patient 01 on 24, 48 and 72 h after co-culture. D-K The percentage of lysed primary GBM cells from indicated patients. All data are represented as the mean ± SEM. *p < 0.05, GD2 4SCAR-T vs. PBMC; #p < 0.05, GD2 4SCAR-T vs. CD19 4SCAR-T (One-Way ANOVA)