Fig. 2
From: Hypoxia and HIFs in Ewing sarcoma: new perspectives on a multi-facetted relationship
Elevated HIF-1-a and GLUT1 expression correlates with worse overall survival in EwS patients. Kaplan–Meier survival analyses in 156 EwS patients based on HIF-1-a and GLUT1 expression levels (cut-off defined as best percentile, log-rank test). Microarray data were retrieved from the Gene Expression Omnibus (accession codes: GSE63157, GSE34620, GSE12102, GSE17618) and normalized using Robust Multiarray Average (RMA) using custom brainarray chip-description files (v20). Batch effects were removed with ComBat. Tumor purity was assessed using the ESTIMATE algorithm. Only samples with a tumor purity > 60% corresponding to The Cancer Genome Atlas (TCGA) standard were included in survival analyses