Fig. 1
From: CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure
Expression of circZBTB44 in RCC tissues and cells. (A) Venn diagram of circZBTB44 (hsa_circ_0002484) upregulated in RCC tumor tissues in GSE100186, GSE108735 and GSE137836. (B) qRT-PCR was used to measure the expression of circZBTB44 in RCC cell lines (A498, OS-RC-2, 769-P, ACHN) and human renal proximal tubular epithelial cell line (HK-2). (C) The Schematic diagram of genomic mapping and splicing patterns of circZBTB44. (D) PCR and electrophoresis analysis was used to detect the existence of circZBTB44 in complementary DNA (cDNA) and genomic DNA (gDNA). (E) CircZBTB44 and ZBTB44 mRNA expression in RCC cells after RNase R treatment was detected using qRT-PCR. (F-G) FISH and subcellular fractionation assays were performed to detect the subcellular location of circZBTB44 in RCC cells. *P < 0.05, ***P < 0.001