Fig. 3
From: CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure
CircZBTB44 interacted with HNRNPC and IGF2BP3 in RCC cells. (A) Venn diagram of RNA binding proteins (RBPs) of circZBTB44 based on the prediction on the RBPsuite website and results of RNA pulldown-MS. (B) The mRNA levels of HNRNPC and IGF2BP3 in circZBTB44 silenced RCC cells. (C) RNA-pulldown assays were performed to detect the interaction of circZBTB44 with HNRNPC or IGF2BP3. (D) RIP assays were used to evaluate the binding between circZBTB44 and HNRNPC or IGF2BP3. (E) The Human Protein Atlas database was used to predict the subcellular localization of HNRNPC and IGF2BP3. (F) The subcellular localizations of HNRNPC and IGF2BP3 were confirmed by immunofluorescence assay. (G) GST-pulldown assays were used to examine the interaction between HNRNPC and IGF2BP3. ***P < 0.001