Fig. 3

circINSIG1-121 promotes CRC proliferation and metastasis. A qRT-PCR analysis of circINSIG1 expression in HCT8 cells with circINSIG1 or circINSIG1-121 overexpression. B Western blot analysis of circINSIG1-121 expression in HCT8 and DLD1 cells with circINSIG1 or circINSIG1-121 overexpression. C Sphere formation assays of circINSIG1 or circINSIG1-121 transfected HCT8 and DLD1 cells, n = 3. Scale bar = 200 μm. D Patient-derived organoids (PDOs) growth with circINSIG1 or circINSIG1-121 transfection, n = 3. Scale bar = 50 μm. E Migration and invasion assays of HCT8 cells with circINSIG1 or circINSIG1-121 overexpression, n = 3. Scale bar = 100 μm. F Sphere formation assays of HCT8 and DLD1 cells circINSIG1 knockdown, n = 3. Scale bar = 200 μm. G PDOs growth with circINSIG1 knockdown, n = 3. Scale bar = 50 μm. H Migration and invasion assays of HCT8 cells with circINSIG1 knockdown, n = 3. Scale bar = 100 μm. I Representative images of gross inspection and H&E staining of the CRC orthotopic tumors (n = 5 per group). Orthotopic tumors scale bars = 5 mm, colon H&E scale bars = 500 μm. J Statistical analysis of orthotopic tumor volumes. K qRT-PCR analysis of human HPRT mRNA relative to mouse 18 S rRNA in the livers of orthotopic xenograft CRC models. Values are represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, by one-way ANOVA