Fig. 5

Chemoresistance of HCT116 IGF2BP2 wildtype (WT) and knockout (KO) cells in 3D cell culture. A-F Spheroid growth of HCT116 WT and HCT116 IGF2BP2 KO cells was monitored for 96 h by automated live-cell microscopy, starting after spheroid formation. Cells were treated with IC50 concentrations of 5-fluorouracil (A, 22.2 µM), oxaliplatin (B, 2.6 µM), gefitinib (C, 23.5 µM), regorafenib (D, 8.1 µM), selumitinib (E, 7.4 µM), nintedanib (F, 8.2 µM) and the vehicle control (Ctrl). Spheroid area was analyzed using the IncuCyte®S3 system and was normalized to 3-day old spheroids. Data are presented as means ± SEM, n = 3 (quadruplicates). Statistical analysis was performed with a two-way ANOVA using the area under the curve. Asterisks represent p values for the comparisons between the untreated and treated condition in the respective cell line. Hashmarks stand for p values labeling differences in growth between both cell lines either control or treated condition, p values were p ≤ 0.05