Fig. 2

Cellular uptake and antiproliferation effect of nanoinhibitor in vitro. A Confocal fluorescent images showing cellular uptake of 5 FAM-nanoinhibitor in CT26 cells at 4, 6, 9, and 12 h. Green fluorescence represents 5-FAM conjugated to nanoinhibitor, and blue fluorescence indicates nucleus (DAPI). B AM/PI staining images of CT26 cells after treated with the nanoinhibitor at the concentrations of 12.50, 25.00, 50.00, and 100.00 mg/L for 24 h. Green fluorescence represents living cells dyed by acetoxymethyl ester (AM), and red fluorescence represents dead cells dyed by propidium iodide (PI). Cell viabilities after different treatments in CT26 cells for 24 h (C), HCT116 cells for 24 h (D), MGC803 cells for 24 h (E), CT26 cells for 48 h (F), HCT116 cells for 48 h (G), and MGC803 cells for 48 h (H). *, EGCG 37 °C vs EGCG 43 °C, ****p < 0.0001. #, Nano 37 °C vs Nano 43 °C, ####p < 0.0001. Nano, nanoinhibitor