Fig. 5

Knock-in GD2-CAR-T cells efficiently control growth of the SY5Y metastatic Neuroblastoma in vivo model. a Schematic of SY5Y tumor cell injection (1 × 10⁶ on day 0 via tail vein injection) in NSG mice. Confirmed tumor engraftment on day 7 via bioluminescent imaging and consecutive GD2-CAR-T cell treatment on day 7 using 5 × 10⁶ GD2-CAR-T cells applied via tail vein injection followed by weekly imaging of tumor bioluminescence. b Bioluminescent images of treated mice over time with color encoded radiance (p/sec/cm²/sr). c Total flux values (p/s) of all animals over time. Statistical significance was evaluated using two-way ANOVA multiple comparisons along with Dunnett’s test for indicated time points. d Weight of all treated animals over time without relevant changes over baseline. f Kaplan–Meier Survival analysis of treated animals. Statistical significance was evaluated using Mantel-Cox test. Error bars indicate SD