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Fig. 7 | Molecular Cancer

Fig. 7

From: A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

Fig. 7

SLU7 regulated the generation of circCAPG. (A) Eight RBPs were selected based on both the previous studies and the TCGA database. (B) The expression level of these RBPs in both tumor and normal tissue. (C) The relative expression level of these eight RBPs in breast cancer cell lines. (D) IB of eight RBPs in MDA-MB-231. (E) qPCR of circCAPG and linear CAPG in RBP OE (left) and KD (right) MDA-MB-231. (F) RIP with an anti-SLU7 and IgG antibody in MDA-MB-231 cell lines was used to detect the mRNA levels of flanking intronic sequences (AluSp, Flam, AluJo, and AluSz) of circCAPG. (G) Illustration of five constructs designed with different combinations of four Alu sequences randomly inserted before exon 6 and/or after exon 8 of circCAPG. (H) qPCR of circCAPG in a, b, c, d, and e constructs. (I) qPCR of circCAPG in a, b, a + OE-Slu7, and b + OE-Slu7. All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598