Fig. 6

CircHAS2 activating bidirectional downstream pathways to promote CRC progression. A The viabilities of SW620 cells following indicated treatments were detected by CCK-8 assays. B Quantitative results and representative images of cell proliferation were conducted by colony formation assay in SW620 cells following indicated treatments. C Quantitative results and representative images of cell proliferation were evaluated by EdU incorporation in SW620 cells following indicated treatments (scale bar: 50 μm). D The percentage of cells in the G1, S, and G2 phases of the entire cell population was determined by flow cytometry following indicated treatments in SW620 cells. E Representative Western blotting images with indicated treatments for p53, p21, CDK2, and CCNE2 proteins in SW620 cells. GAPDH was used as an internal control. F IHC scores of ISH staining (miR-1244) and IHC staining (USP10, p53, p21, CCNE2, and CDK2) assay of tumors of indicated treatments. Cell viability was determined by CCK-8 assay, employing two-way analysis of variance. Statistical differences in clone formation, EdU experiments, and IHC scores were assessed using two-sided Student’s t-test. Data are represented as mean ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significance