Fig. 4

Modulation by IFN-ɣ of PD-L1 expression in NSCLC cell lines carrying different rs822336 genotype. EGFR^mut HCC827^G/G, H1975^G/G, PC-9^G/G and EGFR^wt H460^G/G, A549^G/G, H1299^C/C, H1703^C/C and H1437^C/C cells were seeded into 6-well plates at a density of 2 × 10⁶ cells per well and incubated with IFN-ɣ (100ng/ml). Untreated cells were used as a control. Following a 48 h incubation at 37 °C in a 5% CO₂ atmosphere, cells were harvested and cell surface stained with Allophycocyanin (APC) anti-PD-L1 IgG2b Ab. APC anti-mouse IgG2b was used as a specificity control. Data, expressed as mean fluorescence intensity (MFI), are representative of the results obtained in three independent experiments. No changes in MFI were observed in specificity control following IFN-ɣ incubation (data not shown)