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Fig. 3 | Molecular Cancer

Fig. 3

From: Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation

Fig. 3

CPT-11 induced senescence in CRC cells. CRC cells were treated with or without CPT-11 for 96 h. (A) Representative photographs of SA-β-Gal staining. (B) Quantification of SA-β-Gal-positive cells. (C) Representative photographs of EdU staining. Green fluorescence indicated EdU staining, and blue fluorescence reflected nuclear staining with DAPI. (D) Quantification of EdU-positive cells. (E) Flow cytometry analysis of cell cycle distribution. (F) Quantification of cell cycle distribution. (G) p53 and p21 mRNA levels. (H) p53, p21, and γH2AX protein levels. (I) mRNA levels of SASP factors. Data represented the mean ± standard deviation of at least 3 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001

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Molecular Cancer

ISSN: 1476-4598