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Fig. 4 | Molecular Cancer

Fig. 4

From: Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation

Fig. 4

STCs released a significantly increased number of EVs. (A) The overview scheme of the EVs isolation. (B) Representative TEM images of Ctrl-EVs and Sen-EVs. Ctrl-EVs, EVs derived from untreated CRC cells. Sen-EVs, EVs derived from senescent CRC cells. (C and D) Western blot analysis of EVs markers and endoplasmic reticulum marker in whole cell lysates and EVs. (E) Concentration and size distribution of EVs assessed by NTA. (F) Quantitation of the relative EVs number per cell. The relative number of EVs per cell = (particle size concentration of EVs) × (volume of EVs)/total number of cells. (G) CRC cells were incubated with PKH67-labeled Sen-EVs for 18 h, and the uptaken of Sen-EVs was detected by fluorescence microscopy. Green fluorescence indicated PKH67, and blue fluorescence reflected nuclear staining with Hoechst 33342. Data represented the mean ± standard deviation of at least 3 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001

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Molecular Cancer

ISSN: 1476-4598