Fig. 5

Linc01056 interfered PPARα transcription activity-associated FAO activation. (a) Linc01056 knockdown induced binding of PPARα on to the promoter region of FAO-related genes. (b) Linc01056 knockdown did not change the protein expression of PPARα in HCC cells. (c) Linc01056 located in the cytoplasm of MHCC97L cells with or without sorafenib treatment. (d) Linc01056 specifically bound to PPARα but not PPARγ or FOXO1. (e) PPARα specifically bound to Linc01056 but not other lncRNA like MALAT1. Suppression of PPARα activity by GW6471 (f) reversed the increase of OCR and (g) decrease of ECAR in Linc01056-knockdown HCC cells upon sorafenib treatment. GW6471 inhibited (h) production of mitochondrial ROS, (i) intracellular ATP, (j) cell viability, (k) colonic capacity, and (l) apoptosis of Linc01056-knockdown HCC cells upon sorafenib treatment. *p < 0.05, **p < 0.01, ***p < 0.001