Fig. 1

R-loops are reduced in PCa and recruit IGF2BP proteins. A Isolated R-loops from PCa and paired normal tissues were analyzed by Dot-blot. Methylene blue staining served as a loading control. (Left) representative dot-blot results; (Right) quantification of dot blot (normalized by the density of methylene blue staining). B Regulators were identified by LC-MS/MS analysis using S9.6 antibody in PC-3 cells. C Validation of S9.6 IP LC-MS/MS results by western blot assay. Experiments were performed with Myc-purified IGF2BP proteins and S9.6 antibody in 293T cells. (Left): Representative western blot results. (Right): Quantification of western blot assay. D PC-3 cells were immunostained for IGF2BP1/2/3 and S9.6; representative images are shown (scale bar: 60 μm). E Quantification of cellular immunofluorescence (n = 10 per group). Data are presented as means ± 95CI, two-tailed unpaired t-test. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001