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Fig. 3 | Molecular Cancer

Fig. 3

From: Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer

Fig. 3

The KH domains of IGF2BPs are essential for R-loop accumulation. A PC-3 cells were transfected with wild-type (WT) and KH domain-mutated IGF2BPs variants. The R-loop levels were assessed by dot-blot. B Quantification of dot blot results in B. Data are presented as means ± SD. C RNA pull-down followed by western blot indicated in vitro binding of m6A-modified ssRNA and RNA: DNA hybrid probes with wild-type (WT), KH domain-mutated and RRM domain mutated IGF2BPs variants. D Co-localization of wide-type or mutated IGF2BPs variants with R-loops in IGF2BPs KO PC-3 cells. Scale bar = 60 μm.E Quantification of cellular immunofluorescence (Myc-IGF2BP1 group: n = 10;Myc-IGF2BP2 group: n = 8; Myc-IGF2BP3 group: n = 10). Data are presented as means ± 95CI, two-tailed unpaired t-test. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001

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Molecular Cancer

ISSN: 1476-4598