Fig. 4
YTHDF2 interact with R-loop and YTHDF2 depletion leads to accumulation of R-loops. A PC-3 cell lysates were immunoprecipitated with IgG or anti-S9.6 antibody. Precipitates and input were blotted for YTHDF2. B Pulldown and western blot results showed that YTHDF2 preferentially bind the RNA: DNA hybrids containing m6A-modified RNA. C Pulldown followed by western blot indicated in vitro binding of m6A-modified RNA: DNA hybrid probe with YTHDF2 in control and IGF2BPs overexpression PC-3 cells. D PC-3 cells were transfected with negative control and YTHDF2 siRNA. The R-loop levels were evaluated by dot-blot, and RNaseH1 treated samples were used as control. E Left: Immunofluorescent staining of control and IGF2BP1/2/3 overexpression PC-3 cells. Scale bar = 60 μm. Right: Quantification of cellular immunofluorescence (n = 10 per group). Data are presented as means ± 95CI, two-tailed unpaired t-test. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001