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Fig. 7 | Molecular Cancer

Fig. 7

From: The incorporation of acetylated LAP-TGF-β1 proteins into exosomes promotes TNBC cell dissemination in lung micro-metastasis

Fig. 7

TIP60-driven LAP-TGF-β1 K304 Acetylation Facilitates Lung Metastases Spread. (A) Graphic representation of HA-LAP-TGFB1 protein with domain alterations. Following this, 231 parental cells were co-transfected and subsequently processed with designated antibodies. (B-D) Further explorations of the interactions and modifications of HA-LAP-TGFB1 in response to various treatments. (E) The impact of flag-TIP60 variants on HA-LAP-TGFB1 interactions in 231 LuT3 TGFB1 KO1 cells. (F-G) After treating LuECs with 231 LuT3 TGFB1 KO1-derived exosomes for 24 h, GFP-labeled 231 LuT3 cells were implanted into transwell inserts (n = 5). Scale bars: 200 μm. (F) Extravasation (2 days). (G) Invasion through the basement membrane, followed by the development of a mammosphere (5 days). Data is mean ± SD

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Molecular Cancer

ISSN: 1476-4598