Figure 4

Time course of cell death and morphological appearances of a range of cancers of different histologies exposed to 200 μM THR53. A. Time course of loss of viability in H460 human non-small cell lung cancer, H1299 human non-small cell lung cancer and MCF-7 human breast cancer cells exposed to 200 μM THR53. MRC5-hTERT cells were unaffected by 200 μM THR53. Viability assessed by Trypan Blue exclusion of MRC5-hTERT, H460, H1299 and MCF-7 cells in the presence or absence of the compound, trypsinised off and stained at the appropriate time point. Each data point is the mean of results from triplicate wells (Solid lines - Live cells, Broken lines - Dead Cells; Blue - Control, Red - THR53 200 μM). B. Morphgological appearances of ubiquitous response to 200 μM THR53 of: SK-BR-3 human breast cancer, H460 human non-small cell lung cancer, SW620 human colorectal cancer and G361 malignant melanoma cells. C. Side by side quantification of H460 and MRC-5 cell viability assays at a single, 7 day, time point comparing THR53 (PRGPRP in cyclic amphiphilic cassette) and THR53C (PRRPGP in cyclic amphiphilic cassette) after initial exposure to compounds at a concentration of 200 μM.