Figure 6

THR53 cancer cell killing requires cells to have entered the cell division cycle, does not cause perturbation of cycling cells but does alter relative Cdk1/Cdk4 co-expression. A. Macroscopic appearances of cycling THR53-treated MCF-7 human breast cancer cells in medium + 10% FCS (left hand panels) compared to the same cells held outside the cell cycle in medium + 0.1%FCS (right hand panel) B. DNA histograms of H460 human non-small cell lung cancer cells exposed to THR53 or an autophagy-inducing dose of 50 μM chloroquine. C. Sequential Western blots of Cdk1 and Cdk4 expression in RT112 human bladder cancer cells at 0 - 10 days after exposure to 5 mM linear, end-capped, PRGPRP. D. Western blot of Cdk1 and Cdk4 expression in H460 human non-small cell lung cancer cells 24 hours after exposure to 100 μM and 200 μM THR53.