Figure 6
From: MutS homologue hMSH5: role in cisplatin-induced DNA damage response
The effects of hMSH5 deficiency on cisplatin sensitivity. (A) hMSH5 deficiency increased G2/M arrest in response to cisplatin treatment. 293T, 293T/f-hMSH5, 293T/f-hMSH5Y742F, and 293T hMSH5 RNAi cells (hMSH5-silenced 293T cells) were treated with cisplatin for 2 hrs, and were used for the cell cycle analysis at indicated time points. Percentages of G2/M cells were determined using FlowJo (Dean-Jett model). (B) MTT assays were performed to determine the proliferation of 293T, 293T/f-hMSH5 and 293T/hMSH5Y742F cells in response to cisplatin treatment. (C) Clonogenic survival analysis of 293T, 293T/f-hMSH5, and 293T/f-hMSH5Y742F cells treated with different doses of cisplatin. Cells were treated with indicated doses for 1 hr and maintained in culture for 14 days to allow colony formation. Error bars represent standard deviations from the means of three independent measurements. Asterisks denote p < 0.05 by Student t-test.