Figure 2
From: Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence
Estrogen mediated HIF-1α translocation is sensitive to anti-estrogens and YC-1. TG1-1 cells treated with estradiol (10-8 mol/L) alone show an increase in HIF-1α nuclear accumulation over time (A). Estradiol stimulation was validated using the ERα inhibitor Fulvestrant (ICI). TG1-1 cells were left untreated or treated for 24 hours with estradiol or estradiol plus ICI or estradiol plus the HIF-1α inhibitor YC1 (10-5 mol/L). HIF-1α nuclear accumulation was comparable to control levels when cells were treated with ICI or YC1 (B).