Figure 1
From: In vivo gene transfer targeting in pancreatic adenocarcinoma with cell surface antigens
Oncospecific transduction in human pancreatic tumor cells. PDAC cell surface specific antigens were chosen according to previous reports. They were used to target lentiviral transductions of the Green Fluorescent Protein (GFP) reporter gene, using a Sindbis virus modified glycoprotein. The E2 recognition ZZ domain of glycoprotein has been modified to contain the Fc-binding domain of the protein A. The resulting mutant glycoprotein was called 2.2. Thus, virions can be associated with cell surface-specific antibodies to target the transduction of tumor cells (insert).