Figure 1

TQ induces the phosphorylation of a multitude of proteins. Whole cell lysates of HCT116wt cells treated with 40 μM TQ were probed on PepChip Kinomics v2 peptide array. A. Consensus sequence logo obtained, via WebLogo version 3.3, of the identified protein phosphorylation motifs by Pepscan. In the left panel, the phosphorylated amino acid is placed on the position 0 of the x-axis and the frequency of the neighboring amino acids is represented on the y-axis. In the right panel, the amino acids probability is shown according to their polarity (hydrophilic amino acids (R), hydrophobic amino acids (I) and neutral amino acids (S)). B. Top canonical pathways identified by IPA analysis system of the candidate proteins. C. Function analysis of the different proteins and their scores grouped in networks. D. List of top biofunctions involving the identified proteins with p-value <0.05. E. Venn diagram of the top 50 candidates (≥2 fold phosphorylation) belonging to one or more of the most relevant cancer related biological functions: apoptosis, proliferation and inflammation. The 11 candidates common to all three groups are listed in the red square.