Figure 2

RT-PCR analysis for CUL-1 , -2 , -3 , -4A , -4B , -5 , and ribosomal protein S9 in HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells. RT-PCR was performed using 1 μg of total RNA and 25 cycles of PCR. For all of the cells examined, a 560-bp cDNA for CUL-1, a 358-bp cDNA for CUL-2, a 613-bp cDNA for CUL-3, a 512-bp cDNA for CUL-4A, a 593-bp cDNA for CUL-4B, a 674-bp cDNA for CUL-5, and a 411-bp cDNA for ribosomal protein S9 were amplified by RT-PCR. The results shown are representative of duplicate experiments using independent RNA samples.