Possibility of selection against mtDNA mutations in tumors
© Khaidakov and Reis; licensee BioMed Central Ltd. 2005
Received: 02 February 2005
Accepted: 13 September 2005
Published: 13 September 2005
Several studies of tumors have revealed substantial numbers of clonally expanded somatic mutations in mitochondrial DNA (mtDNA), not observed in adjacent intact tissues. These findings were interpreted as indicating the involvement of mtDNA mutations in tumorigenesis. Such comparisons, however, ignore an important confounding factor: the monoclonal origin of tumors as opposed to the highly polyclonal nature of normal tissues. Analysis of recently published data on the incidence of somatic mutations in nontumor monoclonal cells suggests that, contrary to the prevailing view, the process of tumorigenesis may be accompanied by active selection against detrimental mtDNA mutations.
Accumulation in mutations in mtDNA, leading to an impairment of mitochondrial function, has been implicated in the etiology of aging [1, 2] and of several degenerative pathologies including Parkinson's , Alzheimer's diseases , and diabetes . Similarly, mtDNA mutation is thought to be involved in tumorigenesis based on the presence of novel hetero- and homoplasmies in a number of neoplasms . In the case of degenerative diseases, intracellular clonal expansion of detrimental mtDNA mutations could play a causative role, resulting in cell loss and/or significant functional impairment of affected cells. This logic is not likely to apply to tumorigenesis, and it is not entirely clear at what stage of tumorigenesis a deficiency in aerobic metabolism would confer a selective advantage.
Several different scenarios, linking mtDNA mutations to tumorigenesis, can be envisioned. Cells with an elevated mtDNA mutational load may be more susceptible to carcinogenesis due to increased production of ROS by a dysfunctional electron transport chain, which may in turn promote mutagenesis of nuclear genes. On the other hand, cells with relatively intact oxidative phosphorylation would have enhanced prospects for survival. It is also possible that carcinogenesis is influenced to a larger extent by accumulation of mtDNA mutations in organs distal to the tissue of origin, by creating a systemically permissive environment for development of tumors.
In a number of studies on mtDNA mutations derived from diverse tumors [7–20], the percentage of samples with clonally expanded mtDNA mutations ranged from 27% to almost 80%, averaging 54 ± 5% (mean ± SE; see Suppl. Table 1). These findings led investigators to the conclusion that mtDNA mutations are either more common in carcinogenesis, or in some way predispose to it [8, 21]. It is not possible, however, to infer the functional importance of mitochondrial DNA mutations to tumorigenesis from these comparisons of tumors to adjacent normal tissue. Such studies are confounded by the well-established monoclonal origin of tumors in contrast to the highly polyclonal derivation of normal tissues. Therefore, while the occurrence of hetero- or homoplasmic mutations to mitochondrial DNA is easily detectable in tumor cells, low-level heteroplasmy remains undetected by prevailing methods in essentially all normal tissues. In addition, tumor cells are generally more mitotically active than normal diploid cells  and, as clonal expansion of mutations is thought to result from the random distribution of mutations over multiple replicative cycles , tumors could display higher rates of subclonal development and detection of new heteroplasmies, for this reason alone. Consequently, the validity of direct comparisons between tumor and adjacent normal tissues is questionable, when such studies fail to address the frequency of mitochondrial DNA mutations in individual normal cells or clones of normal cells that have undergone a comparable expansion.
This type of analysis has been recently performed in both dividing and non-dividing normal cells [24–27]. In a study on individual buccal cells and myocytes, direct sequencing of the control region (a non-coding 1121 bp fragment of mtDNA containing several replicational and transcriptional control elements) revealed clonally expanded mutations in 36% of both cell types from older individuals, whereas cells from younger subjects were free from homoplasmies . In another study from the same group, homoplasmy in the D-loop was detected in 20% (5/24) of buccal cells , thus demonstrating that clonal expansion is not a tumor-specific phenomenon. Using available data on tumor dynamics the authors estimated the occurrence of clonal expansions in the entire mtDNA of tumors at 58%, and noted the similarity of this frequency to reported empirical values [8, 21].
The incidence of somatic mutations in the control region of mtDNA is similar for tumors and crypt cells, consistent with these cells having comparable mitotic activity. On the other hand, the ratio of mutation frequencies (per 1 kb) in the D-loop, versus the rest of mtDNA, is much higher in tumors (13.5) than in normal epithelial cells (2.4), suggesting a rather different distribution of mutations or (more likely) very different selective pressures acting on these cell types with respect to mitochondrially encoded subunits. These data support strong selection against detrimental mtDNA mutations in tumor cells, implying that successful tumorigenesis requires intact mitochondria.
- Chomyn A, Attardi G: MtDNA mutations in aging and apoptosis. Biochem Biophys Res Commun. 2003, 304: 519-529. 10.1016/S0006-291X(03)00625-9View ArticlePubMedGoogle Scholar
- Wei YH, Lee HC: Oxidative stress, mitochondrial DNA mutation, and impairment of antioxidant enzymes in aging. Exp Biol Med (Maywood). 2002, 227: 671-682.Google Scholar
- Sherer TB, Betarbet R, Greenamyre JT: Environment, mitochondria, and Parkinson's disease. Neuroscientist. 2002, 8: 192-197.PubMedGoogle Scholar
- Castellani R, Hirai K, Aliev G, Drew KL, Nunomura A: Role of mitochondrial dysfunction in Alzheimer's disease. J Neurosci Res. 2002, 70: 357-360. 10.1002/jnr.10389View ArticlePubMedGoogle Scholar
- Sudoyo H, Suryadi H, Sitorus N, Soegondo S, Pranoto A, Marzuki S: Mitochondrial genome and susceptibility to diabetes mellitus. Adv Exp Med Biol. 2003, 531: 19-36.View ArticlePubMedGoogle Scholar
- Carew JS, Huang P: Mitochondrial defects in cancer. Mol Cancer. 2002, 1: 9- 10.1186/1476-4598-1-9PubMed CentralView ArticlePubMedGoogle Scholar
- Chun-Yang F: personal communication.Google Scholar
- Fliss MS, Usadel H, Caballero OL, Wu L, Buta MR, Eleff SM: Facile detection of mitochondrial DNA mutations in tumors and bodily fluids. Science. 2000, 287: 2017-2019. 10.1126/science.287.5460.2017View ArticlePubMedGoogle Scholar
- He L, Luo L, Proctor SJ, Middleton PG, Blakely EL, Taylor RW: Somatic mitochondrial DNA mutations in adult-onset leukaemia. Leukemia. 2003, 17: 2487-2491. 10.1038/sj.leu.2403146View ArticlePubMedGoogle Scholar
- Liu VW, Shi HH, Cheung AN, Chiu PM, Leung TW, Nagley P: High incidence of somatic mitochondrial DNA mutations in human ovarian carcinomas. Cancer Res. 2001, 61: 5998-6001.PubMedGoogle Scholar
- Nagy A, Wilhelm M, Sukosd F, Ljungberg B, Kovacs G: Somatic mitochondrial DNA mutations in human chromophobe renal cell carcinomas. Genes Chromosomes Cancer. 2002, 35: 256-260. 10.1002/gcc.10118View ArticlePubMedGoogle Scholar
- Parrella P, Xiao Y, Fliss M, Sanchez-Cespedes M, Mazzarelli P, Rinaldi M: Detection of mitochondrial DNA mutations in primary breast cancer and fine-needle aspirates. Cancer Res. 2001, 61: 7623-7626.PubMedGoogle Scholar
- Tan DJ, Bai RK, Wong LJ: Comprehensive scanning of somatic mitochondrial DNA mutations in breast cancer. Cancer Res. 2002, 62: 972-976.PubMedGoogle Scholar
- Tan DJ, Chang J, Chen WL, Agress LJ, Yeh KT, Wang B: Somatic mitochondrial DNA mutations in oral cancer of betel quid chewers. Ann N Y Acad Sci. 2004. 10.1: 310-316.View ArticlePubMedGoogle Scholar
- Wong LJ, Lueth M, Li XN, Lau CC, Vogel H: Detection of mitochondrial DNA mutations in the tumor and cerebrospinal fluid of medulloblastoma patients. Cancer Res. 2003, 63: 3866-3871.PubMedGoogle Scholar
- Hibi K, Nakayama H, Yamazaki T, Takase T, Taguchi M, Kasai Y: Detection of mitochondrial DNA alterations in primary tumors and corresponding serum of colorectal cancer patients. Int J Cancer. 2001, 94: 429-431. 10.1002/ijc.1480View ArticlePubMedGoogle Scholar
- Hibi K, Nakayama H, Yamazaki T, Takase T, Taguchi M, Kasai Y: Mitochondrial DNA alteration in esophageal cancer. Int J Cancer. 2001, 92: 319-321. 10.1002/ijc.1204View ArticlePubMedGoogle Scholar
- Miyazono F, Schneider PM, Metzger R, Warnecke-Eberz U, Baldus SE, Dienes HP: Mutations in the mitochondrial DNA D-Loop region occur frequently in adenocarcinoma in Barrett's esophagus. Oncogene. 2002, 21: 3780-3783. 10.1038/sj.onc.1205532View ArticlePubMedGoogle Scholar
- Tamura G, Nishizuka S, Maesawa C, Suzuki Y, Iwaya T, Sakata K: Mutations in mitochondrial control region DNA in gastric tumours of Japanese patients. Eur J Cancer. 1999, 35: 316-319. 10.1016/S0959-8049(98)00360-8View ArticlePubMedGoogle Scholar
- Okochi O, Hibi K, Uemura T, Inoue S, Takeda S, Kaneko T: Detection of mitochondrial DNA alterations in the serum of hepatocellular carcinoma patients. Clin Cancer Res. 2002, 8: 2875-2878.PubMedGoogle Scholar
- Polyak K, Li Y, Zhu H, Lengauer C, Willson JK: Somatic mutations of the mitochondrial genome in human colorectal tumours. Nat Genet. 1998, 20: 291-293. 10.1038/3108View ArticlePubMedGoogle Scholar
- Michels JJ, Marnay J, Delozier T, Denoux Y, Chasle J: Proliferative activity in primary breast carcinomas is a salient prognostic factor. Cancer. 2004, 100: 455-464. 10.1002/cncr.11916View ArticlePubMedGoogle Scholar
- Coller HA, Bodyak ND, Khrapko K: Frequent intracellular clonal expansions of somatic mtDNA mutations. Ann NY Acad Sci. 2002, 959: 434-447.View ArticlePubMedGoogle Scholar
- Nekhaeva E, Bodyak ND, Kraytsberg Y, McGrath SB, Van Orsouw NJ: Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues. Proc Natl Acad Sci USA. 2002, 99: 5521-5526. 10.1073/pnas.072670199PubMed CentralView ArticlePubMedGoogle Scholar
- Coller HA, Khrapko K, Bodyak ND, Nekhaeva E, Herrero-Jimenez P, Thilly WG: High frequency of homoplasmic mitochondrial DNA mutations in human tumors can be explained without selection. Nat Genet. 2001, 28: 147-150. 10.1038/88859View ArticlePubMedGoogle Scholar
- Taylor RW, Barron MJ, Borthwick GM, Gospel A, Chinnery PF, Samuels DC: Mitochondrial DNA mutations in human colonic crypt stem cells. J Clin Invest. 2003, 112: 1351-1360. 10.1172/JCI200319435PubMed CentralView ArticlePubMedGoogle Scholar
- Shin MG, Kajigaya S, Tarnowka M, McCoy JP, Levin BC, Young NS: Mitochondrial DNA sequence heterogeneity in circulating normal human CD34 cells and granulocytes. Blood. 2004, 103: 4466-4477. 10.1182/blood-2003-11-3949View ArticlePubMedGoogle Scholar
- Bach SP, Renehan AG, Potten CS: Stem cells; the intestinal stem cell as a paradigm. Carcinogenesis. 2000, 21: 469-476. 10.1093/carcin/21.3.469View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.