Identification of novel candidate target genes in amplicons of Glioblastoma multiforme tumors detected by expression and CGH microarray profiling

Table 2 Clones used for validation by FISH.

Gene name	Gene-specific-BAC clones	Location	Control clones	Location
EGFR	RP11-339F13	7p12	p275α7^a	7 cen
CDK4	RP11-571M6	12q13-q14	RP11-467M14	12p13
MDM2	RP11-611O2, RP11-450G15	12q15	RP11-467M14	12p13
ESTs on 13q	RP11-94P17, RP11-406G20	13q32.2
	RP11-364F8, RP11-151A6, RP11-113J24	13q33.3

^a plasmid probe located at the centromere (cen) of chromosome 7. The specificity and location of the probes were confirmed by FISH on normal metaphases before hybridization on the TMAs. When clones were available, and in order to increase the signal, two or three BAC clones around the test gene or EST were hybridized together. Control FISH experiments on normal interphase nuclei were performed beforehand in order to ensure a unique signal when two BAC clones were hybridized together. BAC clones on 13q were purchased from the BACPAC Resources Center (Children's Hospital Oakland Research Institute, CHORI). All other clones were generously provided by Dr. Rocchi.

ISSN: 1476-4598