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Figure 1 | Molecular Cancer

Figure 1

From: Sensitive detection of human papillomavirus type 16 E7-specific T cells by ELISPOT after multiple in vitro stimulations of CD8+ T cells with peptide-pulsed autologous dendritic cells

Figure 1

Immunophenotypic analysis of dendritic cells. Plastic adherent monocytes were cultured in the presence of GM-CSF and IL-4 for 6 days, followed by activation with a cytokine cocktail consisting of IL-1, IL-6, TNF-α and PGE2 for 24 hours (filled histogram). Analysis of typical DC membrane markers (CD80, 83, 86 and HLA-DR) and the monocyte marker CD14 was determined on R1 as shown in the forward vs side scatter dot plot, CD3 and CD19 on the ungated population. An isotype control is represented by the bold line.

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Molecular Cancer

ISSN: 1476-4598