Figure 2From: Bright/ARID3A contributes to chromatin accessibility of the immunoglobulin heavy chain enhancerIn vitro nucleosomal arrays assembled on the IgH enhancer. The rate and extent of the chromatin reconstitution reaction (detailed in Methods and Materials) on the Eμ+MARs template (Fig. 1C) was monitored by formation of nucleosomes. Aliquots were removed at regular 2–240 min intervals, digested with MNase for 5 min, fractionated on a 1.5% agarose gel, and then stained with ethidium bromide. Outside lanes, naked DNA; -ATP lanes, no ATP regeneration system.Back to article page