Figure 7
From: Bright/ARID3A contributes to chromatin accessibility of the immunoglobulin heavy chain enhancer
Complementation of Namalwa with Bright enhances DNase hypersensitivity of chromatin-assembled IgH enhancer. Following in vitro transcription, Eμ+MARs DNA template alone (left panel) or Eμ+MARs reconstituted under Step 1 conditions with either Namalwa nuclear extract alone (middle panel) or Namalwa complemented with Bright (177–601; right panel) were digested with 0, 1.0, 2.0 or 3.0 μg/ml DNase I (lanes 1–4 in each panel). DNA was purified, cut with Bgl II and analyzed by Southern blotting following hybridization with a downstream (XbaI-EcoRI) probe as described in Materials and Methods. Middle and right autoradiographs were exposed ~2.5 times longer so as to better visualize hypersensitive fragments (horizontal bars).