Figure 6From: Cannabinoids reduce ErbB2-driven breast cancer progression through Akt inhibitionAkt downregulation is involved in cannabinoid antitumoral action. (A and B) Viability of N202.1A cells in response to (A) increasing concentrations of THC or JWH-133 or (B) 6 μM THC or 10 μM JWH-133 with or without 2 μM SR141716 (SR1) and/or SR144528 (SR2) for 48 h. Data are expressed as % of vehicle-treated cells, set at 100%. (C) Growth of N202.1A-derived xenografts treated with THC (left panel) or JWH-133 (right panel) with or without SR2. (D) Phospho-Akt and phospho-S6 ribosomal protein (p-S6) levels in N202.1A cells challenged with THC, as determined by Western blot. Total Akt and α-tubulin levels were used for normalization. (E) Phospho-Akt and total Akt levels in N202.1A cells retrovirally transduced with myristoylated Akt (pBABE-Myr-Akt) or the corresponding empty vector (pBABE). (F) Cell viability of pBABE- or myr-Akt-transduced N202.1A cells in response to THC exposure for 72 h. (G and H) Time course of the volume of pBABE-N202.1A-derived (left panels) and myr-Akt-N202.1A-derived (right panels) tumors treated with THC (G), JWH-133 (H) or the corresponding vehicle. *, p < 0.05; **, p < 0.01 vs vehicle-treated cells or tumors; #, p < 0.05; ##, p < 0.01 vs cannabinoid alone-treated cells (B) or vs THC-treated pBABE-transduced cells (F).Back to article page