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Figure 1 | Molecular Cancer

Figure 1

From: Regulation of the Aurora-A gene following topoisomerase I inhibition: implication of the Myc transcription Factor

Figure 1

Topoisomerase I inhibition induced a downregulation of Aurora-A expression. A. HCT116 were synchronized in G1/S with hydroxyurea and released for the indicated times in growth medium complemented with 3% serum. DNA content was analyzed by flow cytometry analysis. B. Aurora-A expression was analyzed in these conditions by western blot (lanes 1-7), or quantitative RT-PCR (B, lanes 8-9) (n = 3 +/- sd). C. HCT116 cells were synchronized in the G2 phase of the cell cycle following treatment with hydroxyurea and serum stimulation for 9hr in growth medium (serum 9 hr) or treated with sn38 (5 ng/ml, 12.5 nM). DNA content was then analyzed by flow cytometry and propidium iodide staining. D-E. Aurora-A expression was measured by western blot analysis (D, lanes 1-4) or quantitative RT-PCR (E) following treatment or cell synchronisation. p21waf1 and tubulin expressions were used as controls (n = 3).

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Molecular Cancer

ISSN: 1476-4598