Figure 1
From: Expression of the neuron-specific protein CHD5 is an independent marker of outcome in neuroblastoma
CHD5 protein expression in normal human neural tissues. CHD5 immunostaining in (A) normal neural tissue, cerebral cortex (100x), box: pyramidal and interneuron CHD5 staining (400x); (B) expression of CHD5 protein detected by immunoblotting in (1) brain cortex total protein, (2) brain cortex nuclear protein fraction, (3) brain cortex cytoplasmatic fraction, (4) LA-N-1 NB cell line total protein and (5) LA-N-1 nuclear fraction; CHD5 predicted molecular weight of 250-260 kDa is based on the amino acid composition (ref. 5); all analyzed samples displayed a 150-160 kDa size band, not yet characterized; (C) Cerebellum (100x), box: Purkinje cell CHD5 staining (400x); (D) Spinal cord (100x), box: Motoneuron CHD5 staining (400x), box*: ependymal cells lining canal spinal cord (100x); (E) Sympathetic ganglia (100x), box: Ganglion cell CHD5 staining (400x); (F) immature neuroblast aggregates within fetal adrenal gland (200x), (arrow) CHD5 immunopositive neuroblasts; (G-H) double fluorescent immunostaining, (G, arrow) NF68+/CHD5+ neuron; (H, arrow) GFAP+/CHD5- astrocyte, (H, arrow head) GFAP-/CHD5+ neuron.