Impact of FHIT loss on the translation of cancer-associated mRNAs

Table 2 mRNAs showing Fhit-dependent changes in ribosomes bound to the 5’-UTR versus coding sequence

Symbol	Gene ID	Ratio of 5’-UTR/CDS (Log₂ fold change)
TBC1D7	NM_016495	4.65
TIAM1	NM_003253	3.61
ZCCHC3	NM_033089	3.33
DCAF5	NM_003861	3.09
ACSL1	NM_001995	3.09
SERTAD3	NM_013368	3.06
TROVE2	NM_004600	2.89
CLPTM1	NM_001294	2.70
HINT2	NM_032593	2.63
FASN	NM_004104	1.19
KPNB1	NM_002265	0.92
CALR	NM_004343	−1.05
RPL37A	NM_000998	−1.71
FBLN1	NM_006486	−1.92
CREBL2	NM_001310	−2.51
MECP2	NM_004992	−2.82
CDKN2C	NM_001262	−3.41
ZNF552	NM_024762	−5.89

RiboDiff was used to determine relative changes in ribosome occupancy of 5’-UTR versus coding region as a function of Fhit expression, using ARD data from Ponasterone A-treated E1 and D1 cells. Only those transcripts with an adjusted p value <0.05 were considered further. The complete list of genes, their relative changes and statistical analysis are included in Additional file 10

ISSN: 1476-4598