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Impact of FHIT loss on the translation of cancer-associated mRNAs

Molecular Cancer volume 16, Article number: 179 (2017) Cite this article

Abstract

Background

FHIT is a genome caretaker/tumor suppressor that is silenced in >50% of cancers. Although it was identified more than 20 years ago, questions remain as to how FHIT loss contributes to cancer, and conversely, how FHIT acts to maintain genome integrity and suppress malignancy. Fhit belongs to the histidine triad family of enzymes that catalyze the degradation of nucleoside 5′,5′-triphosphates, including the m7GpppN ‘caps’ that are generated when mRNAs undergo 3′-5′ decay. This raised the possibility that Fhit loss might affect changes in the translation of cancer-associated mRNAs, possibly as a consequence of increased intracellular concentrations of these molecules.

Results

Ribosome profiling identified several hundred mRNAs for which coding region ribosome occupancy changed as a function of Fhit expression. While many of these changes could be explained by changes in mRNA steady-state, a subset of these showed changes in translation efficiency as a function of Fhit expression. The onset of malignancy has been linked to changes in 5’-UTR ribosome occupancy and this analysis also identified ribosome binding to 5′-untranslated regions (UTRs) of a number of cancer-associated mRNAs. 5’-UTR ribosome occupancy of these mRNAs differed between Fhit-negative and Fhit-positive cells, and in some cases these differences correlated with differences in coding region ribosome occupancy.

Conclusions

In summary, these findings show Fhit expression impacts the translation of a number of cancer associated genes, and they support the hypothesis that Fhit’s genome protective/tumor suppressor function is associated with post-transcriptional changes in expression of genes whose dysregulation contributes to malignancy.

Background

‘Fragile’ sites are among the most frequently deleted loci in cancers [1]. FHIT was identified more than 20 years ago [2] at a locus that is deleted or otherwise silenced in >50% of most human cancer types [3, 4]. Loss of FHIT alleles occurs early in malignant transformation [5, 6], and is associated with decreased apoptosis [7], increased EMT [8, 9], increased resistance to genotoxic agents [10], and altered control of reactive oxygen species production [11]. Nevertheless, the mechanisms through which Fhit protein affects these functions have remained elusive.

Fhit is a small cytoplasmic protein that does not interact with known tumor suppressors or oncogenes. Its name (Fragile Histidine Triad) derives from a His-X-His-X-His-XX motif characteristic of nucleoside hydrolases. Fhit cleaves diadenosine triphosphate (Ap3A) in vitro to yield ADP and AMP [12], and Ap3A accumulates in Fhit-deficient cells [13]. Recently Taverniti and Seraphin [14] identified Fhit as a scavenger decapping enzyme. Scavenger decapping enzymes are responsible for degrading m7GpppN cap dinucleotides that are generated by 3′-5’ mRNA decay. Because they can be bound by eIF4E such mRNA decay remnants can inhibit translation initiation if they accumulate to a level that can compete with mRNA 5′ ends. DcpS is the major scavenger decapping enzyme [15], and the identification of Fhit as another of this type of enzyme is consistent with previous work describing Fhit’s ability to cleave GpppBODIPY, an mRNA cap-like molecule [16].

Translation plays a critical role in cancer [17], and a recent report showed that, for a number of cancer-associated mRNAs, changes in ribosome occupancy of upstream open reading frames precedes the appearance of detectable tumors [18]. As noted above, the key biochemical property of Fhit is its ability to hydrolyze nucleoside 5′,5′-triphosphates, and while catalytically-inactive forms of Fhit are unable to function as tumor suppressors, a mutant (H96N) that binds to but does not hydrolyze nucleoside 5′,5′-triphosphates is nearly as effective as wild-type Fhit in suppressing tumor formation [12]. FHIT allele loss can occur prior to appearance of detectable preneoplastic lesions or tumors [5, 6, 19]. Since few direct targets of Fhit loss have been identified [11, 20], we wondered if its tumor suppressor or genome caretaker effects might be related to its function in degrading nucleoside 5′,5′-triphosphates, and to the impact of these molecules on the translation of a limited number of mRNAs.

The current study used Fhit expression-negative H1299 lung cancer cells carrying an inducible FHIT transgene to examine the impact of Fhit protein and its loss on the scope of translating mRNAs. Ribosome profiling and RNA-Seq showed that Fhit loss is associated with changes in steady-state level and ribosome occupancy of several hundred mRNAs, but little change in translation efficiency of most of the transcriptome. Rescuing Fhit expression resulted in changes in average ribosome density of a limited number of cancer-associated genes and this was reflected by changes in protein expression. We also identified additional cancer-associated genes for which 5’-UTR ribosome occupancy changed when Fhit expression was restored. This result is consistent with transformation-associated changes in 5’-UTR ribosome occupancy in [18] and with loss of Fhit expression as an early step in this process.

Methods

Ribosome profiling, RNA-seq, and informatics

Detailed description of the methods used for ribosome profiling and data analysis are in Additional file 1.

Cell culture

The engineered H1299 lung carcinoma cells, which inducibly express Fhit cDNA and protein (D1) or an empty vector (E1) have been described [20]. H1299 cells were maintained in DMEM medium with 10% FBS, zeocin, gentamicin and geneticin. Fhit expression was induced by addition of ponasterone A (2 μM) (Life Technologies) to the growth medium for 48 h.

Preparation of cell extracts and RNA

Cytoplasmic extracts were prepared and harvested as described in [21]. Briefly, H1299 E1 and D1 cells (1.75 × 106 cells) were seeded into 150 mm dishes. 24 h later, the cells were induced with ponasterone A (2 μM) for 48 h. Cells were washed twice with PBS, scraped, and pelleted by centrifugation at 2500 xg for 5 min at 4 °C. Subsequent procedures were carried out on ice. Cell pellets were lysed in 5 pellet volumes of ice cold cytoplasmic lysis buffer (50 mM Tris-HCl, pH 7.5, 10 mM KCl, 10 mM MgCl2, 150 mM NaCl, 0.2% NP-40, 2 mM dithiothreitol (DTT), 0.5 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM sodium orthovanadate, supplemented with 5 μl/ml RNAseOUT (Life Technologies), 25 μl/ml protease inhibitor cocktail (Sigma), 10 μl/ml each phosphatase inhibitor cocktails 2 and 3 (Sigma)). Lysates were incubated on ice for 10 min with mild agitation every 2 min. Nuclei and other debris were pelleted with a 10 min centrifugation at 4 °C and 16,100 xg. Pellets were discarded and supernatants were reserved and used for western blotting (see below) or RNA extraction. Cytoplasmic RNA was harvested from the supernatants using a Direct-Zol (Zymo) kit according to the manufacturer’s Reaction Clean-Up protocol. Whole cell extracts were prepared as above, but nuclei were disrupted with three 10 s pulses (5 min rest on ice between each pulse) using a Fisher Scientific 60 sonic dismembrator fitted with a microtip on setting 3 prior to the final centrifugation step.

Western blotting

SDS Laemmli loading dye was added to cytoplasmic or whole cell extracts harvested as above and samples were heated at 95 °C for 5 min. Proteins were separated by SDS gel electrophoresis, transferred to Immobilon FL (EMD millipore) PVDF membranes, and immunoblotted with antisera against human Fhit (1:1000, [22], human eEEF2 (1:1000, One World Labs), human TP53I3 (1:1000, One World Labs), human IFIT1 (1:600, Protein-Tech), human Vinculin (1: 5000, Abcam), human ADAM9 (1:1000, GeneTex) and human FASN (1:1000, Proteintech). IR-680 or IR-800 conjugated goat anti-rabbit or anti-mouse secondary antibodies were used at 1:5000 dilutions and blots were scanned and bands analyzed using a Licor Odyssey Imager.

Reverse transcription and quantitative PCR (qPCR)

For each sample, one microgram of cytoplasmic RNA was spiked with 0.1 ng of Luciferase control RNA (Promega), and reverse transcribed using Superscript III (Life Technologies) with random hexamers according to the manufacturer’s instructions. cDNA was assayed using a CFX Connect Real-Time System (BioRad) for qPCR. Briefly, the cDNA from RT-PCR was diluted 10-fold and qPCR reactions were carried out using SensiFAST SYBR No-ROX reagent (Bioline) following the manufacturer’s protocol for a 2-step reaction. Predesigned Primetime qPCR primer pairs for GAPDH (Hs.PT.39a.22214836), eEF2 (Hs.PT.58.40025410), TP53i3 (Hs.PT.58.27248188), IFIT1 (Hs.PT.56a.20769090.g) and custom primer pairs for Luciferase and Vinculin were purchased from Integrated DNA Technologies (IDT). Primer sequences and ordering information are listed in Additional file 2. Primer efficiencies were determined using a standard curve from serial dilutions and efficiency was calculated using Efficiency = −1 + 10(−1/slope). The comparative ΔΔCt method (2-ΔΔCq) was used to calculate the relative mRNA levels (normalized against the spiked luciferase and an endogenous control (GAPDH) transcript) in empty vector (E1) and Fhit-expressing (D1) cells. All qPCR assays were performed in triplicate using cDNA reverse transcribed from independent biological triplicate RNA samples.

uORF predictions

The sequences of the entire 5’-UTR and coding region were analyzed using the seqshoworfs command within MATLAB’s (Ver: 8.1.0.60) Bioinformatics Toolbox (Ver: 4.3), with the “AlternativeStartCodon” search parameter set to true, to look for start codons: ATG, GTG, CTG, TTG; reading frames: 1, 2, and 3 on the direct strand only; and searched for uORFs with a minimum length of 10 codons using the standard genetic code.

Results

Ribosome profiling identifies a set of mRNAs whose translation is controlled by Fhit

The approach used to determine the transcriptome-wide impact of Fhit expression on translation is shown in Fig. 1a. The E1 cells are Fhit-negative H1299 cells stably transfected with empty vector (E1 cells), and the D1 cells carry a Ponasterone A-inducible FHIT transgene. Prior to generating sequencing libraries we confirmed that treatment with Ponasterone A induced Fhit expression in the D1 cell line but not the E1 cell line (Fig. 1b). Duplicate cultures of each line were then processed for RNA-Seq and ribosome profiling (RIBO-Seq) [23], with all data subjected to Benjamini Hochberg False Discovery Rate correction. Scatter plots showed good agreement between duplicate RNA-Seq libraries of E1 and D1 cell lines (Additional file 3). Fhit expression had limited overall impact on the transcriptome, with most mRNAs showing no statistically significant change in their steady-state level (Fig. 1c). Using a 1.5-fold change as cutoff (red circles) 209 transcripts increased and 377 decreased significantly in Fhit-positive vs Fhit-negative cells. The identities and changes in the abundance of these mRNAs are listed in Additional file 4.

Fig. 1
figure 1

Identification of mRNAs whose levels and translation are altered by Fhit. a The workflow for identifying mRNAs under translational control by Fhit is shown. E1 cells are H1299 cells with a stably transfected empty vector and D1 cells are H1299 cells with a stably transfected, ponasterone A-inducible FHIT transgene. Duplicate cultures of each cell line were treated with Ponasterone A, and cytoplasmic extracts were used for RNA-Seq and ribosome profiling (RIBO-Seq). b Fhit induction in D1 but not E1 cells was confirmed by Western blotting. c The log2 change in steady state levels of the mRNA transcriptome is shown as a function of RNA-Seq read counts. All mRNAs that underwent a statistically-significant change in association with Fhit expression (p < 0.05) are indicated with red circles, and we arbitrarily selected a 1.5-fold change as a cutoff for further study. These transcripts and their fold changes are listed in Additional file 4. d The log2 fold change in coding region RIBO-Seq for each mRNA is shown as a function of RNA-Seq read counts. Statistically significant changes are indicated by red circles as in c. These transcripts and their fold changes are listed in Additional file 7. e RiboDiff was used to normalize the coding region RIBO-Seq data in d to the RNA-Seq data in c. Fhit-mediated changes in the resulting Average Ribosome Density (ARD) are plotted as a function of RNA-Seq read counts. Again a cutoff of 1.5-fold was selected. mRNAs that underwent a statistically-significant change with Fhit (p < 0.05) are labeled and indicated with red dots

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Scatter plots of RIBO-Seq data also showed good agreement between duplicate D1 and E1 RIBO-Seq libraries (Additional file 5). The quality of these libraries was further supported by a metagene analysis of ribosome protected fragments that showed the expected triplet phasing near the start and stop codons and a peak 13 nucleotides upstream of start codons characteristic of ribosomes bound to this site (Additional file 6). Fhit expression also significantly impacted the quantity of ribosome-bound transcripts (Fig. 1d), with 67 transcripts showing increased and 103 transcripts showing decreased ribosome occupancy with Fhit expression. The identities and changes in ribosome occupancy of these mRNAs are listed in Additional file 7.

The FHIT-induced changes observed by RIBO-Seq could result from changes in translation efficiency, steady-state mRNA levels, or a combination of those factors. To decipher this, the RIBO-Seq data for the coding regions were normalized to RNA-Seq data using RiboDiff [24]. The resulting Average Ribosome Density (ARD), accurately tracks translation efficiency of each mRNA [25]. Scatter plots showed good agreement between duplicates of Fhit-positive (D1) and Fhit-negative (E1) cells (Additional file 8), which were then used to compare coding region ARD between treatment groups (Fig. 1e). This analysis identified 6 mRNAs with a statistically-significant increased coding region ARD in Fhit-positive vs Fhit-negative cells, and 4 mRNAs with decreased coding region ARD. These mRNAs and the degrees to which coding region ARD changed with Fhit are presented in the top half of Table 1.

Table 1 mRNAs showing Fhit-dependent changes in ribosome loading
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Association of FHIT loss with changes in coding region ribosome occupancy

Plotting ribosome distribution across each of these mRNAs identified positional changes in ribosome occupancy as one mechanism by which Fhit loss/Fhit expression affects ARD (Fig. 2). CDKN2C mRNA has a short (506 nt) coding region and a long (1216 nt) 5’-UTR that includes several putative upstream open reading frames (uORFs). In Fhit negative cells CDKN2C showed little evidence for CDS ribosome occupancy; however, ribosomes were present on the 5’-UTR. 5’-UTR ribosome occupancy decreased with Fhit expression and this was accompanied by the appearance of CDS-bound ribosomes, suggesting one or more features of the CDKN2C 5’-UTR are responsible for the Fhit-mediated increase in translation (see below). Similar results were also observed for ATG16L2 and MECP2. In Fhit-negative cells there was little evidence for translating ribosomes on the CDS of IGSF9 and TP53I3, and for both of these mRNAs Fhit expression resulted in the appearance of bound ribosomes. TP53I3 was unique in having stalled ribosomes at the initiation codon that disappear and are replaced by ribosomes that distribute across the entire coding region in Fhit-expressing cells. Taken together, these data support a role for Fhit in maintaining the translation of a limited set of mRNAs.

Fig. 2
figure 2

Fhit-mediated changes in ribosome distribution across mRNAs identified by coding region ARD. a Ribosome distribution is shown across the mRNAs identified in Table 1 as having increased coding region ARD in Fhit-positive (blue) vs Fhit-negative (red) cells. b Ribosome distribution is shown across the mRNAs identified in Table 1 as having decreased coding region ARD in Fhit-positive (blue) vs Fhit-negative (red) cells. The coding region of each mRNA is shaded

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RiboDiff analysis also identified a number of mRNAs whose ARD was elevated in Fhit-negative versus Fhit-positive cells, the most abundant of which was EEF2 (Fig. 2b). Importantly, EEF2 is elevated in lung squamous cell carcinoma and elevated levels of EEF2 drive cell proliferation and promote EMT [26].

Validation of ARD targets

To confirm that the preceding results reflect changes in protein expression 3 of the targets in Table 1 were selected (on the basis of antibody availability) for evaluation by Western blotting and RT-qPCR. Western blotting showing TP53I3 was 4.2-fold higher in Fhit-positive vs Fhit-negative cells. This was similar to the 4.5-fold difference in ARD determined by RiboDiff (Fig. 3a). In Fhit-expressing cells EEF2 was 1.5-fold lower by Western blotting and 1.7-fold lower by RiboDiff. In contrast, IFIT1 was 1.6-fold lower by Western blotting and 5.2-fold lower by RiboDiff. The reason for this difference is not known. Vinculin expression was unchanged in both RNA-Seq and RIBO-Seq (Additional files 4 and 7), and quantitative changes for each of these proteins were determined by normalizing to Vinculin.

Fig. 3
figure 3

Fhit-mediated changes in target protein expression are independent of mRNA level. a D1 and E1 cells treated for 24 h with Ponasterone A were analyzed for changes in TP53I3, IFIT1, EEF2 and Vinculin. Shown are representative Western blots for each of triplicate determinations and the fold change normalized to Vinculin is presented beneath the panels for TP53I3, IFIT1 and EEF2. b Cytoplasmic extracts from these cells were spiked with luciferase RNA as a control for sample recovery and analyzed by RT-qPCR for changes in steady-state levels of TP53I3, EEF2, IFIT1 and Vinculin mRNA. The mean fold change ± SEM (n = 3) determined for each mRNA by by RT-qPCR (black bars) is shown alongside the average difference from the duplicate RNA-Seq datasets. c The analysis in a was repeated for ADAM9 and FASN, with U2AF35 used as a normalization control

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We also looked for Fhit-mediated differences in the corresponding mRNAs by RT-qPCR and compared these results with those obtained by RNA-Seq (Fig. 3b). Although TP53I3 mRNA was somewhat higher in Fhit-positive versus Fhit-negative cells (~1.5-fold higher by RT-qPCR and ~1.2-fold higher by RNA-Seq) this was significantly less than the increase in TP53I3 protein determined by Western blotting and ARD, and by the changes in ribosome distribution seen in Fig. 2a. Although IFIT protein was lower in Fhit-positive cells IFIT1 mRNA was higher (1.8-fold higher by RT-qPCR, 1.6-fold higher by RNA-Seq), and EEF2 protein was ~1.5 lower but EEF2 mRNA was either unchanged by RNA-Seq or slightly lower by RT-qPCR. Taken together the preceding data confirm that Fhit affects the expression of a limited number of proteins through changes in the translation of their corresponding mRNAs.

Impact of Fhit expression on 5’-UTR ribosome occupancy

The identification of 5’-UTR bound ribosomes on Fhit-regulated mRNAs was unanticipated and suggested a possible link between Fhit-mediated changes in gene expression and cancer-associated changes in 5’-UTR ribosome binding [18]. RiboDiff analysis of 5’-UTRs identified 5 mRNAs with increased 5’-UTR ribosome occupancy associated with Fhit expression, and 2 mRNAs with decreased 5’-UTR ribosome occupancy (bottom half of Table 1, Fig. 4). Importantly, each of these has one or more predicted uORFs within its 5’-UTR (Additional file 9). These changes ranged from a 13.6-fold increase for ACSL1 to a 4-fold decrease for RPL37A. In some cases differences in 5’-UTR ribosome occupancy were also associated with differences in CDS ribosome occupancy, an example of which is ADAM9. ADAM9 encodes a precursor that is processed to the mature protein, and Western blotting confirmed both forms were higher in Fhit-expressing cells (Fig. 3c).

Fig. 4
figure 4

Fhit-mediated changes in ribosome distribution across mRNAs identified by changes in 5’-UTR ARD. a Ribosome distribution is shown across the mRNAs identified as having increased 5’-UTR ARD in Fhit-positive (blue) vs Fhit-negative (red) cells. b Ribosome distribution is shown across the mRNAs identified as having decreased 5’-UTR ARD in Fhit-positive (blue) vs Fhit-negative (red) cells. The 5’-UTR of each mRNA is shaded

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Fhit expression affects the relative representation of 5’-UTR vs coding region-bound ribosomes

Several of the genes that were identified in Fig. 1e also showed reciprocal differences in 5’-UTR ribosome binding. To determine the extent of this effect RiboDiff data were mined for changes in the ratio of 5’-UTR-bound ribosomes vs CDS-bound ribosomes (Additional file 10). This identified 19 genes (p < 0.05) for which the ratio of 5’-UTR/CDS ribosome occupancy either increased or decreased as a function of Fhit expression (Table 2 and Fig. 5). Several of these, including CDKN2C, ACSL1, TROVE2, MECP2 and RPL37A were identified earlier (see Figs. 2 and 4). The remaining genes fall into four groups; mRNAs whose 5’-UTR and CDS occupancy increased in Fhit-positive cells (Fig. 6a), mRNAs showing increased 5’-UTR but little change in CDS ribosome occupancy (Fig. 6b), and mRNAs with decreased 5’-UTR ribosome occupancy (Fig. 6c and d). Although FASN coding region ribosome occupancy was unchanged the level of the encoded protein was slightly higher in Fhit-positive cells (Fig. 3c).

Table 2 mRNAs showing Fhit-dependent changes in ribosomes bound to the 5’-UTR versus coding sequence
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Fig. 5
figure 5

Identification of mRNAs with Fhit-mediated changes in ribosome occupancy of 5’-UTR versus coding region. RiboDiff was used to determine ribosome occupancy of 5’-UTRs vs the coding region. The results of which were used in Additional file 11 to identify mRNAs for which Fhit expression altered the ratio between these features. These data were plotted as a function of RIBO-Seq read counts, with those mRNAs from Additional file 11 showing statistically-significant changes identified by name and as red dots

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Fig. 6
figure 6

Fhit changes the ribosome occupancy ratio of 5’-UTR versus coding region. Ribosome distribution is shown across the mRNAs identified in Table 2 as having changes to 5’-UTR/coding region ribosome occupancy in Fhit-positive (blue) vs Fhit-negative (red) cells. Omitted are mRNAs from Table 2 whose ribosome distribution is presented in Fig. 4. The 5’-UTR of each mRNA is shaded. a Five mRNAs showed increased ribosome loading in both the 5’-UTR and the coding region, but the increases observed in the 5’-UTR were larger than those observed in the coding region. b Four mRNAs exhibited an increase in 5’-UTR-bound ribosomes while their coding regions remained essentially unchanged. c Two mRNAs showed decrease 5’-UTR ribosome occupancy in Fhit-positive versus Fhit-negative cells, but increased coding region ribosome occupancy. d Two mRNAs showed decreased 5’-UTR ribosome occupancy in Fhit-positive versus Fhit-negative cells but little change in coding region ribosome occupancy

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Discussion

Despite more than 1100 papers on Fhit the molecular mechanism(s) by which it acts to affect these processes are poorly understood. We approached this from the perspective that Fhit might act indirectly to alter gene expression as a function of its ability to clear nucleoside 5′,5′-triphosphates and act as a scavenger decapping enzyme [14]. We reasoned that Fhit loss might increase the intracellular levels of these dinucleotides, including the m7G caps generated by mRNA 3′-5′ decay, and these in turn might affect the translation of some mRNAs. To address this we performed ribosome profiling of Fhit-deficient H1299 lung cancer cells carrying an inducible FHIT transgene and a matching cell line carrying empty vector. Changes in Fhit expression impacted ribosome occupancy, with 67 mRNAs showing statistically-significantly increased ribosome binding and 103 mRNAs showing decreased binding (Fig. 1d and Additional file 7). For the most part changes in ribosome occupancy could be accounted for by corresponding changes in mRNA level. Once this was taken into account 6 mRNAs were identified for which Fhit increased coding region average ribosome density (ARD) and 4 mRNAs for which ARD decreased (Fig. 1e, Table 1).

This small number of mRNAs was unexpected, but their identity was significant. Of the mRNAs showing increased translation in association with Fhit expression, CDKN2C is a member of the INK4 cyclin-dependent kinase inhibitors with medium to low expression in lung cancers (see [27, 28]. IGSF9 is involved in cellular adhesion, TP53I3 is induced by TP53 and thought to generate reactive oxygen molecules, MECP2 binds methyl-CpG sites in chromatin and CSRP2 is a LIM domain protein thought to be involved in regulating cell growth. Mecp2 is also the key protein that is lost or mutated in Rett syndrome [29]. We also identified a number of mRNAs for which coding region ribosome occupancy was higher in Fhit-negative versus Fhit-positive cells. The greatest differential was seen for EEF2, a GTP-binding protein that catalyzes the movment of peptide-bound tRNAs from the ribosome A site to the P site during translation. This is notable because EEF2 is highly expressed in numerous cancers [30], where it promotes cell proliferation and EMT [26].

Because we were interested in the relationship between Fhit loss and protein expression our initial work focused on coding region ribosome occupancy. However, this changed with the report by Sendoel et al. [18], showing the onset of malignancy is preceded by changes in 5’-UTR ribosome occupancy of a number of cancer-associated mRNAs. This was first evident in Fig. 2, where CDKN2C, ATG16L2 and MECP2 had higher 5’-UTR ribosome occupancy in Fhit-negative vs Fhit-positive cells. Restoring Fhit expression shifted this pattern, resulting in higher coding region and lower 5’-UTR ribosome occupancy of these mRNAs. A related picture was seen for TP53I3, but in Fhit-negative cells there appears to be a single stalled ribosome bound at the start codon that is released by Fhit expression. These findings are consistent with results in [31] and [32] in which a uORF regulates downstream translation, which in our case is affected by the presence or absence of Fhit.

Futher examination identified 5 mRNAs for which changes in 5’-UTR ribosome occupancy increased with Fhit rescue, 2 mRNAs for which 5’-UTR ribosome occupancy decreased (Fig. 4), and 13 mRNAs for which Fhit expression changed the ratio of 5’-UTR vs coding region ribosome occupancy. Each of these mRNAs has a potential or verified uORF, the sequences of which are listed in Additional file 9. As in [18], these uORFs have mostly non-canonical (CUG, GUG, UUG) start codons, and some extend into the coding region, raising the possibility that loss of Fhit leads to changes in ribosome occupancy that can affect the protein products translated from these mRNAs.

In total, this study identified 30 genes for which FHIT loss alters ribosome occupancy of the 5’-UTR or the coding region of their respective mRNAs (Table 3). These are notable for the breadth of pathways they impact. A number of genes have been directly linked to malignancy, including ADAM9, CDKN2C, DCAF5, KPNB1, MECP2, RACK1, SERTAD3, TBC1D7, TP53I3 and TIAM1. H2AF7 was recently identified as a major regulator of EMT [33]. Others that have been indirectly linked to cancer include HIST1H2AD, two zinc finger proteins (ZCCHC3, ZNF552), and CSRP2, CREBL2, and SERTAD3. Intermediary metabolism and protein synthesis have essential roles in malignancy, and these processes are represented in our dataset by ACSL1 and FASN, which encode enzymes that function in fatty acid synthesis and lipid metabolism, and TROVE2, EEF2, RACK1 and RPL37A, each of which impact RNA metabolism or translation. This analysis also identified HINT2, which is a histidine triad containing protein that functions in mitochondrial cell death signaling. Based on results presented here we propose that past challenges in identifying how loss of FHIT leads to cancer may be partially due to the fact that Fhit and its loss exert pleiotropic effects on the translation of genes that function in a multitude of cellular processes.

Table 3 Targets of Fhit-mediated changes in ribosome occupancy
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It remains to be determined how Fhit or its loss affects changes in translation. The 5’-UTR is a nexus for post-transcriptional gene regulation [34], and with changes in 5’-UTR ribosome occupancy being the feature most characteristic of the targets identified here. We speculate that sequences or structural elements within this region play a major role in determining specificity for their regulation by Fhit. Fhit does not contain any recognizable RNA binding domains, nor has it been identified as a constituent of the mRNP proteome [35,36,37]. As a member of the histidine triad family Fhit catalyzes the hydrolysis of nucleoside 5′,5′-triphosphates, including m7G caps that are the remnants of 3′-5’ mRNA decay [14]. The H1299 cells used here have low levels of the main scavenger decapping enzyme DcpS, and we hypothesize that nucleoside 5′,5′-triphosphates (including but not necessarily limited to m7G caps) accumulate in Fhit-negative cells to a level sufficient to compete with eIF4E binding to the capped ends of a limited number of mRNAs. As noted above, sequences or structural elements within the 5’-UTR likely determine susceptibility to this disruption. Indirect targeting of gene expression by the intracellular level of nucleoside 5′,5′-triphosphates is consistent with the observation that Fhit/H96N, which binds nucleoside 5′,5′-triphosphates with high affinity, is nearly as effective as wild-type Fhit in suppressing tumor formation [38, 39].

Conclusions

In summary, results presented here show that FHIT loss is associated with changes in ribosome occupancy of the 5’-UTR and/or coding region of 30 different mRNAs, many of which are associated with cancer. This is consistent with recent findings showing changes in 5’-UTR occupancy of a number of cancer-associated genes precedes appearance of a detectable malignancy. The protein products of Fhit-regulated mRNAs function in a number of different pathways, the diversity of which may help explain the challenges encountered in identifying how FHIT loss leads to genome instability and cancer.

Abbreviations

ARD:

Average ribosome density

EMT:

Epithelial-mesenchyal transition

uORF:

Upstream open reading frame

UTR:

Untranslated region

References

  1. Bignell GR, Greenman CD, Davies H, et al. Signatures of mutation and selection in the cancer genome. Nature. 2010;463:893–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell. 1996;84:587–97.

    Article  CAS  PubMed  Google Scholar 

  3. Pichiorri F, Palumbo T, Suh SS, et al. Fhit tumor suppressor: guardian of the preneoplastic genome. Future Oncol. 2008;4:815–24.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Waters CE, Saldivar JC, Hosseini SA, Huebner K. The FHIT gene product: tumor suppressor and genome “caretaker”. Cell Mol Life Sci. 2014;71:4577–87.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gorgoulis VG, Vassiliou LV, Karakaidos P, et al. Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature. 2005;434:907–13.

    Article  CAS  PubMed  Google Scholar 

  6. Bartkova J, Horejsí Z, Koed K, et al. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature. 2005;434:864–70.

    Article  CAS  PubMed  Google Scholar 

  7. Sard L, Accornero P, Tornielli S, et al. The tumor-suppressor gene FHIT is involved in the regulation of apoptosis and in cell cycle control. Proc Natl Acad Sci U S A. 1999;96:8489–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Joannes A, Grelet S, Duca L, et al. Fhit regulates EMT targets through an EGFR/Src/ERK/slug signaling axis in human bronchial cells. Mol Cancer Res. 2014;12:775–83.

    Article  CAS  PubMed  Google Scholar 

  9. Suh SS, Yoo JY, Cui R, et al. FHIT suppresses epithelial-mesenchymal transition (EMT) and metastasis in lung cancer through modulation of microRNAs. PLoS Genet. 2014;10:e1004652.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Ottey M, Han SY, Druck T, et al. Fhit-deficient normal and cancer cells are mitomycin C and UVC resistant. Br J Cancer. 2004;91:1669–77.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Okumura H, Ishii H, Pichiorri F, Croce CM, Mori M, Huebner K. Fragile gene product, Fhit, in oxidative and replicative stress responses. Cancer Sci. 2009;100:1145–50.

    Article  CAS  PubMed  Google Scholar 

  12. Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleoside 5′,5″’-P1, P3-triphosphate hydrolase. Biochemistry. 1996;35:11529–35.

    Article  CAS  PubMed  Google Scholar 

  13. Murphy GA, Halliday D, McLennan AG. The Fhit tumor suppressor protein regulates the intracellular concentration of diadenosine triphosphate but not diadenosine tetraphosphate. Cancer Res. 2000;60:2342–4.

    CAS  PubMed  Google Scholar 

  14. Taverniti V, Seraphin B. Elimination of cap structures generated by mRNA decay involves the new scavenger mRNA decapping enzyme Aph1/FHIT together with DcpS. Nucleic Acids Res. 2014;43:482–92.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Li Y, Kiledjian M. Regulation of mRNA decapping. Wiley Interdiscip Rev RNA. 2010;1:253–65.

    Article  PubMed  Google Scholar 

  16. Draganescu A, Hodawadekar SC, Gee KR, Brenner C. Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates. J Biol Chem. 2000;275:4555–60.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Pelletier J, Graff J, Ruggero D, Sonenberg N. Targeting the eIF4F translation initiation complex: a critical nexus for cancer development. Cancer Res. 2015;75:250–63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Sendoel A, Dunn JG, Rodriguez EH, et al. Translation from unconventional 5′ start sites drives tumour initiation. Nature. 2017;541:494–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Sozzi G, Pastorino U, Moiraghi L, et al. Loss of FHIT function in lung cancer and preinvasive bronchial lesions. Cancer Res. 1998;58:5032–7.

    CAS  PubMed  Google Scholar 

  20. Saldivar JC, Miuma S, Bene J, et al. Initiation of genome instability and preneoplastic processes through loss of Fhit expression. PLoS Genet. 2012;8:e1003077.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kiss DL, Waters CE, Ouda IM, et al. Identification of Fhit as a post-transcriptional effector of thymidine kinase 1 expression. Biochim Biophys Acta. 2017;1860:374–82.

    Article  CAS  PubMed  Google Scholar 

  22. Druck T, Hadaczek P, TB F, et al. Structure and expression of the human FHIT gene in normal and tumor cells. Cancer Res. 1997;57:504–12.

    CAS  PubMed  Google Scholar 

  23. Ingolia NT, Brar GA, Rouskin S, McGeachy AM, Weissman JS. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc. 2012;7:1534–50.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Zhong Y, Karaletsos T, Drewe P, et al. RiboDiff: detecting changes of mRNA translation efficiency from ribosome footprints. Bioinformatics. 2017;33:139–41.

    Article  PubMed  Google Scholar 

  25. Balakrishnan R, Oman K, Shoji S, Bundschuh R, Fredrick K. The conserved GTPase LepA contributes mainly to translation initiation in Escherichia Coli. Nucleic Acids Res. 2014;42:13370–83.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Song Y, Sun B, Hao L, et al. Elevated eukaryotic elongation factor 2 expression is involved in proliferation and invasion of lung squamous cell carcinoma. Oncotarget. 2016;7:58470–82.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Human protein atlas. https://www.proteinatlas.org. Accessed December 1, 2017.

  28. Uhlén M, Björling E, Agaton C, et al. A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteomics. 2005;4:1920–32.

    Article  PubMed  Google Scholar 

  29. Tillotson R, Selfridge J, Koerner MV, et al. Radically truncated MeCP2 rescues Rett syndrome-like neurological defects. Nature. 2017;550:398–401.

    Article  CAS  PubMed  Google Scholar 

  30. Oji Y, Tatsumi N, Fukuda M, et al. The translation elongation factor eEF2 is a novel tumor-associated antigen overexpressed in various types of cancers. Int J Oncol. 2014;44:1461–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Palam LR, Baird TD, Wek RC. Phosphorylation of eIF2 facilitates ribosomal bypass of an inhibitory upstream ORF to enhance CHOP translation. J Biol Chem. 2011;286:10939–49.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Barbosa C, Romão L. Translation of the human erythropoietin transcript is regulated by an upstream open reading frame in response to hypoxia. RNA. 2014;20:594–608.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Domaschenz R, Kurscheid S, Nekrasov M, Han S, Tremethick DJ. The Histone variant H2A.Z is a master regulator of the epithelial-Mesenchymal transition. Cell Rep. 2017;21:943–52.

    Article  CAS  PubMed  Google Scholar 

  34. Hinnebusch AG, Ivanov IP, Sonenberg N. Translational control by 5′-untranslated regions of eukaryotic mRNAs. Science. 2016;352:1413–6.

    Article  CAS  PubMed  Google Scholar 

  35. Kwon SC, Yi H, Eichelbaum K, et al. The RNA-binding protein repertoire of embryonic stem cells. Nat Struct Mol Biolf. 2013;20:1122–30.

    Article  CAS  Google Scholar 

  36. Castello A, Fischer B, Eichelbaum K, et al. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell. 2012;149:1393–406.

    Article  CAS  PubMed  Google Scholar 

  37. Castello A, Fischer B, Frese CK et al. Comprehensive identification of RNA-binding domains in human cells. Mol cell. 2016.

    Google Scholar 

  38. Siprashvili Z, Sozzi G, Barnes LD, et al. Replacement of Fhit in cancer cells suppresses tumorigenicity. Proc Natl Acad Sci U S A. 1997;94:13771–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Pace HC, Garrison PN, Robinson AK, et al. Genetic, biochemical, and crystallographic characterization of Fhit-substrate complexes as the active signaling form of Fhit. Proc Natl Acad Sci U S A. 1998;95:5484–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Not applicable

Funding

This research was supported by a multi-PI IDEA grant from the Ohio State University Comprehensive Cancer Center Pelotonia fund (to KH, DS, RB) and from grants from the National Institute of General Medical Science (grant number GM084177, DRS), and the National Science Foundation (grant number DMR-1410172, RB). DLK was supported by a Pelotonia postdoctoral fellowship and training grant T32 CA0093338 from the National Cancer Institute. The OSU Genomics Shared Resource is supported by a grant from the National Cancer Institute (grant number P30CA016058) to The Ohio State University Comprehensive Cancer Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of Pelotonia, The Ohio State University, the National Institutes of Health or the National Science Foundation.

Availability of data and materials

The raw data (FASTQ files) are available online under BioProject (https://www.ncbi.nlm.nih.gov/bioproject/) accession number PRJNA390535.

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Author notes

    Authors and Affiliations

    1. Center for RNA Biology, The Ohio State University, Columbus, OH, 43210, USA

      Daniel L. Kiss, Ralf Bundschuh & Daniel R. Schoenberg

    2. Comprehensive Cancer Center, The Ohio State University, Columbus, OH, 43210, USA

      Daniel L. Kiss, Kay Huebner & Daniel R. Schoenberg

    3. Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, 43210, USA

      Daniel L. Kiss & Daniel R. Schoenberg

    4. Department of Physics, The Ohio State University, Columbus, OH, 43210, USA

      William Baez & Ralf Bundschuh

    5. Department of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, 43210, USA

      Kay Huebner

    6. Department of Chemistry & Biochemistry, The Ohio State University, Columbus, OH, 43210, USA

      Ralf Bundschuh

    7. Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH, 43210, USA

      Ralf Bundschuh

    8. Biomarker Research Program, Houston Methodist Research Institute, Houston, TX 77030, USA

      Daniel L. Kiss

    Authors
    1. Daniel L. Kiss
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    Contributions

    DLK performed all the cell culture and molecular biology work, including generating the ribosome profiling libraries, isolating RNA for RNA-Seq and RT-qPCR and Western blotting. He also participated in generating text, tables, and figures of the manuscript. WB performed bioinformatics analysis of RNA-Seq and RIBO-Seq data, generated figures and tables from RNA-Seq and RIBO-Seq data, performed analysis of cancer-associated gene products, generated most of the data in Additional files, generated text of the manuscript. KH provided information and insight into the relationship of Fhit to cancer, provided cell lines and antibodies used in this study, generated text of the manuscript. RB supervised WB’s approach to data analysis, participated in all aspects of experiment planning and informatics analysis, participated in weekly strategy and planning meetings, generated text of the manuscript. DRS oversaw planning and experimental approach, supervised DLK’s experimental design and analysis, participated in weekly strategy and planning meetings, prepared the bulk of the manuscript, generated all of the figures. All authors read and approved the final manuscript.

    Corresponding author

    Correspondence to Daniel R. Schoenberg.

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    Additional files

    Additional file 1:

    Detailed presentation of methods used for ribosome profiling and bioinformatics analysis. (PDF 84 kb)

    Additional file 2:

    A list of oligonucleotides used. (XLSX 11 kb)

    Additional file 3:

    Scatterplots of duplicate RNA-Seq libraries from Fhit-deficient (E1) and Fhit-expressing (D1) H1299 cells. (PDF 688 kb)

    Additional file 4:

    Excel file listing all of the transcripts identified by RNA-Seq as having increased or decreased steady-state as a function of Fhit expression. These are ordered by fold change and statistical significance. (XLSX 110 kb)

    Additional file 5:

    Scatterplots of duplicate ribosome profiling libraries from Fhit-deficient (E1) and Fhit-expressing (D1) H1299 cells. (PDF 639 kb)

    Additional file 6:

    Metagene analysis showing the 3 nucleotide periodicity of bound ribosomes. (PDF 333 kb)

    Additional file 7:

    Excel file listing all of the transcripts identified by RIBO-Seq as having increased or decreased ribosome occupancy as a function of Fhit expression. These are ordered by fold change and statistical significance. (XLSX 25 kb)

    Additional file 8:

    Scatterplots of average ribosome density of duplicate Fhit- negative (E1) and Fhit-expressing (D1) H1299 cells. (PDF 770 kb)

    Additional file 9:

    Upstream open reading frames within the 5’-UTRs of Fhit-regulated mRNAs. (XLSX 27 kb)

    Additional file 10:

    Transcripts with Fhit-mediated changes in the relative occupancy of 5’-UTR/coding sequence. (XLSX 1582 kb)

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    Cite this article

    Kiss, D.L., Baez, W., Huebner, K. et al. Impact of FHIT loss on the translation of cancer-associated mRNAs. Mol Cancer 16, 179 (2017). https://doi.org/10.1186/s12943-017-0749-x

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    • DOI: https://doi.org/10.1186/s12943-017-0749-x

    Keywords

    • Fhit
    • Translational control
    • Ribosome profiling
    • Scavenger decapping
    • Gene expression

    Molecular Cancer

    ISSN: 1476-4598