Fig. 4
From: RNA-based therapies: A cog in the wheel of lung cancer defense
Schematic illustrations for mRNA-based LC vaccine development. The aim of mRNA vaccine development was started with a comparative analysis of exome of LC tissue and normal tissue to identify potential tumor-specific antigen(s). The detailed analysis coupled with high throughput methods enables the verification of identified antigen(s)/neoantigen(s) specific to LC. The mRNA sequence(s) specific to tumor antigens will then be synthesized, modified, and cloned into appropriate plasmids for the mRNA transcription. The liposome formulations (or other appropriate vehicles) of the final, optimized mRNA(s) encoding LC-specific antigens will be injected into LC patients to elicit a prominent anticancer immune response for the destruction of LC tumors