Skip to main content

Correction to: Ponatinib efficiently kills imatinib-resistant chronic eosinophilic leukemia cells harboring gatekeeper mutant T674I FIP1L1-PDGFRα: roles of Mcl-1 and β-catenin

The Original Article was published on 28 January 2014

Correction to: Mol Cancer 13, 17 (2014)

https://doi.org/10.1186/1476-4598-13-17

Following publication of the original article [1], minor errors were identified in the images presented in Figs. 1 and 3; specifically:

  • Fig. 1d: immunoblot band for p-Erk1/2 in BaF3-T674I FIP1L1-PDGFRα cells has been replaced with the correct image

  • Fig. 3e: immunoblot bands for Bim in both BaF3-WT FIP1L1-PDGFRα and BaF3-T674I FIP1L1-PDGFRα cells have been replaced with the correct images

The corrected figures are given below. The correction does not have any effect on the results or conclusions of the paper. The original article has been corrected.

Fig. 1
figure 1

Ponatinib inhibits phosphorylation of PDGFRα and its downstream signaling molecules. A BaF3-T674I FIP1L1-PDGFRα cells exhibited differential sensitivity to ponatinib and sorafenib. BaF3-T674I FIP1L1-PDGFRα cells were treated with the TKIs at the indicated concentrations for 24 h, and the levels of phosphorylated and total PDGFRα were detected with the relevant antibodies. B Ponatinib inhibited phosphorylation of PDGFRα in a concentration-dependent manner. EOL-1 and BaF3-WT or -T674I FIP1L1-PDGFRα cells were exposed to escalating concentrations of ponatinib for 24 h. C Ponatinib inhibited phosphorylation of PDGFRα in a time-dependent manner. The concentrations of ponatinib were 1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells, respectively. D Ponatinib concentration-dependently inhibited phosphorylation of Stat3, Stat5, Akt and Erk1/2. The cells were exposed to increasing concentrations of ponatinib for 24 h. E Ponatinib time-dependently inhibited phosphorylation of Stat3, Stat5, Akt and Erk1/2. 300 nM ponatinib was applied.

Fig. 3
figure 2

Ponatinib induces apoptosis in FIP1LI-PDGFRα-expressing cells. A EOL-1 and BaF3-WT or -T674I FIP1L1-PDGFRα cells were exposed to increasing concentrations of ponatinib for 24 h, apoptotic cells were assayed with flow cytometry by PI/Annexin V-FITC (EOL-1) or 7-AAD/Annexin V-PE (BaF3-WT or -T674I FIP1L1-PDGFRα cells) staining. Left, representative histograms; Right, statistical data of 3 independent experiments, the vertical axis stands for the sum of all dead cells. Error bars represent 95% confidence intervals. **, P < 0.01; ***, P < 0.0001, one-way ANOVA, post hoc comparisons, Tukey’s test. B The indicated cells were treated with or without ponatinib (1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells, respectively) for 24 h, washed with PBS and fixed with 2% glutaraldehyde plus 2% paraformaldehyde in 0.1 M cacodylate buffer (pH 7.3). Representative photographs (9700×) were acquired under transmission electron microscopy. C The concentration- (for 24 h) and time-dependent (1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells) cleavage of PARP and caspase-3 triggered by ponatinib was analyzed by immunoblotting. D Ponatinib elicited release of AIF and cytochrome c into the cytosol. Cells were treated with 1 nM ponatinib for the indicated durations and the cytosolic fraction was extracted with digitonin buffer. Levels of AIF and Cytochrome c (Cyto c) were detected by immunoblotting. E Immunoblotting of apoptosis-related proteins in CEL cells after treatment for 24 h

Reference

  1. Jin Y, Ding K, Li H, et al. Ponatinib efficiently kills imatinib-resistant chronic eosinophilic leukemia cells harboring gatekeeper mutant T674I FIP1L1-PDGFRα: roles of Mcl-1 and β-catenin. Mol Cancer. 2014;13:17. https://doi.org/10.1186/1476-4598-13-17.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jingxuan Pan.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Jin, Y., Ding, K., Li, H. et al. Correction to: Ponatinib efficiently kills imatinib-resistant chronic eosinophilic leukemia cells harboring gatekeeper mutant T674I FIP1L1-PDGFRα: roles of Mcl-1 and β-catenin. Mol Cancer 20, 137 (2021). https://doi.org/10.1186/s12943-021-01407-6

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/s12943-021-01407-6