- Short communication
- Open Access
POU1F1 is a novel fusion partner of NUP98 in acute myeloid leukemia with t(3;11)(p11;p15)
- Susana Lisboa†1,
- Nuno Cerveira†1,
- Susana Bizarro1,
- Cecília Correia1,
- Joana Vieira1,
- Lurdes Torres1,
- José M Mariz2 and
- Manuel R Teixeira1, 3Email author
© Lisboa et al.; licensee BioMed Central Ltd. 2013
- Received: 12 September 2012
- Accepted: 3 January 2013
- Published: 18 January 2013
NUP98 gene rearrangements have been reported in acute myeloid leukemia, giving rise to fusion proteins that seem to function as aberrant transcription factors, and are thought to be associated with poor prognosis.
A patient with treatment-related acute myeloid leukemia presented a t(3;11)(p11;p15) as the only cytogenetic abnormality. FISH and molecular genetic analyses identified a class 1 homeobox gene, POU1F1, located on chromosome 3p11, as the fusion partner of NUP98. In addition, we have found that the patient harbored an FLT3-ITD mutation, which most likely collaborated with the NUP98-POU1F1 fusion gene in malignant transformation.
We have identified POU1F1 as the NUP98 fusion partner in therapy-related AML with a t(3;11)(p11;p15). This is the first POU family member identified as a fusion partner in human cancer.
- NUP98 gene
- Gene fusion
- Acute leukemia
Acute myeloid leukemia (AML) is often associated with chromosomal translocations, resulting in fusion genes that have implications in disease prognosis and treatment. Chromosomal translocations involving the NUP98 gene have been reported in a wide range of hematopoietic malignancies, involving more than 20 different partner genes to generate fusion proteins with abnormal function . The frequency of these rearrangements in AML is 1 to 2% and they seem to be associated with poor prognosis, thus highlighting the relevance of identifying and characterizing cases harboring such genetic alterations [1, 2].
The NUP98 gene codes for a protein that is a component of the nuclear pore complex (NPC) and contains multiple nontandem GLFG repeats (Gly-Leu-Phe-Gly) that are thought to function as docking sites to allow the bidirectional transport of mRNA and proteins between the nucleus and the cytoplasm . The NUP98 protein is also involved in cell cycle progression, mitotic spindle formation, and gene transcription . All the translocations so far described involving the NUP98 gene result in the fusion of its 5′ region (coding for the GLFG repeats) to the 3′ region of the partner gene. The C-terminal partners can be divided into two general classes: homeodomain (HD) proteins and non-HD proteins . HD proteins contain a DNA-binding domain (the HD domain) that is in all instances retained in the fusion protein, with its amino terminal region replaced by the GLFG repeats of NUP98. The fusion protein seems to function as an aberrant transcription factor, directly binding DNA to activate gene transcription and leading to the deregulation of HOXA cluster genes that are important for normal hematopoietic differentiation [1, 3].
A 57-year-old female was diagnosed with a breast adenocarcinoma in 2001 (T1N0M0; treated with radical mastectomy, followed by four courses of chemotherapy with 5-fluorouracil, epirubicin, and cyclophosphamide, radiotherapy and hormonotherapy with tamoxifen). In 2005, the patient developed leucocytosis associated with asthenia and febrile syndrome and the diagnosis of therapy-related AML was established (AML-M4 according to the French-American-British classification). Blood count was hemoglobin 11.0 g/dL, platelets 102 × 109/L, and leukocytes 71 × 109/L with 13% circulating blasts and the bone marrow was infiltrated with 41.3% blasts. She was treated with chemotherapy (cytarabine, daunorubicin, and cyclosporin) and a complete response was attained. The patient was proposed to bone marrow transplantation, but no compatible donor was found. Ten months later the patient showed evidence of relapse with leukocytosis and thrombocytopenia and died within five months.
For the identification of the genomic breakpoints of the NUP98-POU1F1 fusion, several primers were designed in NUP98 intron 11 and POU1F1 intron 4 (Additional file 1). When the NUP98_Fint11H sense primer was used in combination with the POU1F1_Rint4A and POU1F1_Rint4B antisense primers, amplification products of 412 bp and 603 bp were observed, respectively (Figure 2D). Partial sequencing of the amplification products showed that the breakpoint was located 7490 bp downstream of NUP98 exon 11 and, interestingly, within POU1F1 exon 4, 129 bp downstream of the start of POU1F1 exon 4, and no evidence of mutation or deletion was detected in the breakpoint region (Figure 2A and F). This leads to retention in the genomic sequence of 36 nucleotides from POU1F1 exon 4 that are not included in the mature NUP98-POU1F1 messenger RNA, probably as a result of the removal of the splice acceptor site of POU1F1 intron 3.
Since FLT3-ITD mutations have been reported in more than 50% of the patients with NUP98- HOX fusions , we have searched for this abnormality in our patient using the FLT3 Mutation Assay for Gel Detection (InVivo Scribe Technologies, San Diego, USA) according to the manufacturer’s instructions. We found an internal tandem mutation of the FLT3 gene, as shown by the presence of an amplification product of approximately 350 bp (Additional file 2: Figure S1).
To our knowledge, this is the first time that POU1F1, a POU class 1 homeobox gene, is reported as being involved in a fusion gene in human cancer. POU1F1 belongs to the POU family of transcription factors that plays a fundamental role in inhibition and promotion of cell differentiation, as well as in the determination of cell lineage and regulation of cell migration, survival and terminal differentiation . In adults, POU1F1 is expressed in cells of the anterior pituitary gland, where it plays a role in cellular commitment, differentiation and proliferation, driving the expression of growth hormone, prolactin, and thyroid-stimulating hormone β chain genes . POU1F1 expression has also been reported in hematopoietic and lymphoid tissues , and its expression was correlated with increased cellular proliferation in breast cancer and human myeloid leukemic cells, leading to the suggestion that POUF1 may be involved in the regulation of cellular proliferation [8–10]. Since the expression of NUP98- HOX chimeric genes seems to be under the control of the NUP98 promoter , leading to overexpression of the HD transcription factor, it is expected that the same occurs in the rearrangement we here describe, and that POU1F1 overexpression might result in increased proliferation of leukemic cells.
Several lines of evidence have suggested that many of the NUP98 fusion proteins can act as aberrant transcription factors. It seems that a synergistic action between NUP98 and the HD partner gene leads to the creation of a unique protein with unique DNA targeting properties and function, which can lead to leukemogenic transformation . Indeed, the NUP98 chimeric proteins not only retain the N-terminal sequences that are responsible for both DNA binding and transcription activation through a “cryptic” transactivation domain [11–13], but also the C-terminal region of the HD partner can directly bind DNA and activate gene transcription . Furthermore, mouse models of NUP98-HOX fusions were shown to induce leukemia with variable latency , which was associated with deregulation of HOXA cluster genes that are thought to play a key role in normal hematopoietic differentiation . Impaired terminal differentiation of hematopoietic cells is a hallmark of leukemia and, according to the two-hit model of leukemogenesis, is classified as a type II mutation . However, this model of leukemic transformation, although overly simplified, requires the presence of a concomitant mutation leading to increased proliferation, survival, or both (type I mutation) . It seems that NUP98- HOX fusions have the ability to initiate and maintain a state of self-renewal necessary, but not sufficient, for the development of leukemia . Indeed, type I mutations are common in NUP98 rearranged leukemia, including mutations in the NRAS, KRAS, KIT, WT1 and FLT3 genes [5, 16]. The type I mutation in the case we here present was the FLT3-ITD mutation, which we hypothesize collaborated with the NUP98-POU1F1 fusion in malignant transformation.
In summary, we have identified POU1F1 as the NUP98 fusion partner in therapy-related AML with a t(3;11)(p11;p15). This is the first POU family member identified as a fusion partner in leukemia, and further studies are necessary to uncover the precise role played by this family of genes in this disease.
This work was supported by a grant from the “Associação Portuguesa Contra a Leucemia” (2006–30.2.AP/MJ) and “Liga Portuguesa Contra o Cancro”.
- Gough SM, Slape CI, Aplan PD: NUP98 gene fusions and hematopoietic malignancies: common themes and new biologic insights. Blood. 2011, 118: 6247-6257. 10.1182/blood-2011-07-328880PubMed CentralView ArticlePubMedGoogle Scholar
- Nebral K, König M, Schmidt HH, Lutz D, Sperr WR, Kalwak K, Brugger S, Dworzak MN, Haas OA, Strehl S: Screening for NUP98 rearrangements in hematopoietic malignancies by fluorescence in situ hybridization. Haematologica. 2005, 90: 746-752.PubMedGoogle Scholar
- Eklund E: The role of Hox proteins in leukemogenesis: insights into key regulatory events in hematopoiesis. Crit Rev Oncog. 2011, 16: 65-76. 10.1615/CritRevOncog.v16.i1-2.70PubMed CentralView ArticlePubMedGoogle Scholar
- Shaffer LG, Slovak ML, Campbell LJ: An International System for Human Cytogenetic Nomenclature. 2009, , BaselKarger Publishers.Google Scholar
- Chou WC, Chen CY, Hou HA, Lin LI, Tang JL, Yao M, Tsay W, Ko BS, Wu SJ, Huang SY: Acute myeloid leukemia bearing t(7;11)(p15;p15) is a distinct cytogenetic entity with poor outcome and a distinct mutation profile: comparative analysis of 493 adult patients. Leukemia. 2009, 23: 1303-1310. 10.1038/leu.2009.25View ArticlePubMedGoogle Scholar
- Andersen B, Rosenfeld MG: POU domain factors in the neuroendocrine system: lessons from developmental biology provide insights into human disease. Endocr Rev. 2011, 22: 2-35.Google Scholar
- Delhase M, Vergani P, Malur A, Hooghe-Peters EL, Hooghe RJ: The transcription factor Pit-1/GHF-1 is expressed in hematopoietic and lymphoid tissues. Eur J Immunol. 1993, 23: 951-955. 10.1002/eji.1830230428View ArticlePubMedGoogle Scholar
- Ben-Batalla I, Seoane S, Garcia-Caballero T, Gallego R, Macia M, Gonzalez LO, Vizoso F, Perez-Fernandez R: Deregulation of the Pit-1 transcription factor in human breast cancer cells promotes tumor growth and metastasis. J Clin Invest. 2010, 120: 4289-4302. 10.1172/JCI42015PubMed CentralView ArticlePubMedGoogle Scholar
- Ben-Batalla I, Seoane S, Macia M, Garcia-Caballero T, Gonzalez LO, Vizoso F, Perez-Fernandez R: The Pit-1/Pou1f1 transcription factor regulates and correlates with prolactin expression in human breast cell lines and tumors. Endocr Relat Cancer. 2010, 17: 73-85. 10.1677/ERC-09-0100PubMed CentralView ArticlePubMedGoogle Scholar
- Costoya JA, García-Barros M, Gallego R, Señarís R, Arce VM, Devesa J: Correlation of Pit-1 gene expression and Pit-1 content with proliferation and differentiation in human myeloid leukemic cells. Exp Cell Res. 1998, 245: 132-136. 10.1006/excr.1998.4232View ArticlePubMedGoogle Scholar
- Capelson M, Liang Y, Schulte R, Mair W, Wagner U, Hetzer MW: Chromatin-bound nuclear pore components regulate gene expression in higher eukaryotes. Cell. 2010, 140: 372-383. 10.1016/j.cell.2009.12.054PubMed CentralView ArticlePubMedGoogle Scholar
- Kalverda B, Pickersgill H, Shloma VV, Fornerod M: Nucleoporins directly stimulate expression of developmental and cell-cycle genes inside the nucleoplasm. Cell. 2010, 140: 360-371. 10.1016/j.cell.2010.01.011View ArticlePubMedGoogle Scholar
- Xu S, Powers MA: Nup98-homeodomain fusions interact with endogenous Nup98 during interphase and localize to kinetochores and chromosome arms during mitosis. Mol Biol Cell. 2010, 21: 1585-1596. 10.1091/mbc.E09-07-0561PubMed CentralView ArticlePubMedGoogle Scholar
- Argiropoulos B, Humphries RK: Hox genes in hematopoiesis and leukemogenesis. Oncogene. 2007, 26: 6766-6776. 10.1038/sj.onc.1210760View ArticlePubMedGoogle Scholar
- Kosmider O, Moreau-Gachelin F: From mice to human: the “two-hit model” of leukemogenesis. Cell Cycle. 2006, 5: 569-570. 10.4161/cc.5.6.2577View ArticlePubMedGoogle Scholar
- Taketani T, Taki T, Nakamura T, Kobayashi Y, Ito E, Fukuda S, Yamaguchi S, Hayashi Y: High frequencies of simultaneous FLT3-ITD, WT1 and KIT mutations in hematological malignancies with NUP98-fusion genes. Leukemia. 2010, 24: 1975-1977. 10.1038/leu.2010.207View ArticlePubMedGoogle Scholar
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