- Open Access
Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
Molecular Cancervolume 13, Article number: 74 (2014)
PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the Notch pathway and its involvement in PC progression.
Stable PTOV1 knockdown or overexpression were performed by lentiviral transduction. Protein interactions were analyzed by co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous gene expression was analyzed by real time RT-PCR and/or Western blotting. Exogenous promoter activities were studied by luciferase assays. Gene promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). In vivo studies were performed in the Drosophila melanogaster wing, the SCID-Beige mouse model, and human prostate cancer tissues and metastasis. The Excel package was used for statistical analysis.
Knockdown of PTOV1 in prostate epithelial cells and HaCaT skin keratinocytes caused the upregulation, and overexpression of PTOV1 the downregulation, of the Notch target genes HEY1 and HES1, suggesting that PTOV1 counteracts Notch signaling. Under conditions of inactive Notch signaling, endogenous PTOV1 associated with the HEY1 and HES1 promoters, together with components of the Notch repressor complex. Conversely, expression of active Notch1 provoked the dismissal of PTOV1 from these promoters. The antagonist role of PTOV1 on Notch activity was corroborated in the Drosophila melanogaster wing, where human PTOV1 exacerbated Notch deletion mutant phenotypes and suppressed the effects of constitutively active Notch. PTOV1 was required for optimal in vitro invasiveness and anchorage-independent growth of PC-3 cells, activities counteracted by Notch, and for their efficient growth and metastatic spread in vivo. In prostate tumors, the overexpression of PTOV1 was associated with decreased expression of HEY1 and HES1, and this correlation was significant in metastatic lesions.
High levels of the adaptor protein PTOV1 counteract the transcriptional activity of Notch. Our evidences link the pro-oncogenic and pro-metastatic effects of PTOV1 in prostate cancer to its inhibitory activity on Notch signaling and are supportive of a tumor suppressor role of Notch in prostate cancer progression.
The PTOV1 gene and protein are expressed at increased levels in PC [1–4] and other tumors [5, 6]. PTOV1 expression is detected in putative pre-neoplastic lesions of atypical adenomatous hyperplasia (AAH)  and its detection in pre-neoplastic high-grade prostate intraepithelial neoplasia (HGPIN) lesions from prostatic biopsies may be helpful in the early diagnosis of PC . The protein consists of a tandem repeated domain, also present as a single copy in PTOV2, or MED25, a subunit of the Mediator transcriptional complex [1, 9, 10], conserved among higher eukaryotes, that uses novel structural modes to recruit the VP16 activation domain [11–13]. Recently, PTOV1 was shown to repress the MED25-mediated transcription of the retinoic acid (RA) receptor , suggesting a potential molecular mechanism underlying resistance to RA . Additionally, PTOV1 may interact with the lipid-raft associated protein Flotillin-1 , the phosphoserine-recognizing protein 14-3-3σ , the BUZ/Znf-Ubp domains of the Histone deacetylase HDAC6 , and the ribosomal protein RACK1 . Although it is difficult to ascertain how each of these interactions contributes to a possible role of dysregulated PTOV1 expression in cancer progression, this protein modulates cell proliferation, cell cycle progression [4, 16], protein synthesis and gene transcription [15, 19, 20]. Combined these observations suggest a function for PTOV1 as an adaptor protein implicated in different cellular events and locations.
Here we report a functional interaction of PTOV1 with the Notch signaling pathway. Notch is part of an evolutionarily conserved pathway that regulates cell differentiation, proliferation and growth . Following ligand binding, two subsequent proteolytic cleavages by intracellular γ-secretase release the active intracellular domain of Notch (ICN) from the cell membrane. ICN translocates to the nucleus and interacts with the CBF-1/RBP-Jκ transcription factor and directs the expression of numerous downstream target genes, including HES1 and HEY1[22–25]. In the absence of ICN, CBF-1/RBP-Jκ acts as a transcriptional repressor by forming a complex that includes SMRT/NCoR, and HDAC1 .
In cancer, Notch signaling, initially shown to be oncogenic in human T cell acute lymphoblastic leukemia (T-ALL), and later in other tumors [27–29], was subsequently found to function also as a suppressor of tumor growth, depending on cell lineage or tissue [30–32]. In PC, several evidences suggest a tumor suppressor role of Notch signaling , including its action in promoting PTEN activity , the downregulation of Notch1 and HEY1 expression in tumors [34, 35], the undetectable levels of Notch1 and ligands in PC cell lines, and the inhibition of PC cell proliferation by ICN . However, additional findings, including the elevated levels of Notch ligand Jagged1 in PC and its association to recurrence, the requirement of Notch2 in the resistance to docetaxel, and the Notch1 association with aggressive PC, are suggestive of an oncogenic role [37–39].
In this work, we show that PTOV1 promotes the invasion and anchorage-independent growth of prostate cancer cells while it acts as a novel repressor of the Notch target genes HES1 and HEY1. Reciprocally, a constitutively activated Notch1 receptor decreases anchorage-independent growth and invasion in vitro. In vivo, PTOV1 antagonizes Notch function in the Drosophila melanogaster wing, and it is required for full tumor growth and metastatic potentials of PC-3 prostate cancer cells in an immunodeficient mouse model. In prostate tumors, the reciprocal expression patterns observed for PTOV1 and Notch targets support our in vitro findings.
PTOV1 blunts Notch transcriptional activity
The nuclear localization of PTOV1 was previously associated with higher proliferative index and tumor grade , suggesting a link between nuclear PTOV1 and cancer progression in different tumor types, including prostate and bladder cancers. Others have shown that, in the nucleus, PTOV1 antagonizes the transcriptional activity of complexes requiring the histone acetyl-transferase CBP . Although CBP was reported to function as a classic tumor-suppressor gene in the mouse and in prostate cancer [40–43], other evidences have also suggested a role in promoting cell proliferation and prostate cancer progression [44, 45]. We thus searched for interactions of PTOV1 with transcriptional networks known to participate in the progression of PC and other cancers. Notch is one such major signaling pathway, regulating the formation of the normal prostate and involved in PC [36, 46, 47].
To confirm that prostate cells have active Notch signaling , RWPE1 cells, derived from benign prostate epithelium, and PC-3 prostate cancer cells were treated with the γ-secretase inhibitor DAPT, known to prevent Notch processing and transcriptional signaling . This treatment caused a significant downregulation of the endogenous Notch target genes HES1 and HEY1, as determined by real-time RT-PCR (Figure 1A) and a comparable decline in the HES1 promoter activity, as determined by luciferase transactivation assays (Figure 1A). A similar reduction in HES1- luciferase promoter activity was observed after the expression of a dominant negative form of MAML1, a transcriptional co-activator of the Notch signaling pathway . Similar results were obtained with LNCaP prostate cancer cells (Additional file 1: Figure S1). Expression analysis of the four Notch receptors shows that prostate cell lines have moderate and variable levels of Notch2, Notch3 and Notch4, while Notch1 is expressed at lower levels in metastatic cell lines (Additional file 1: Figure S2). Together, these observations suggest that Notch maintains at least in part the transcription levels of HES1 and HEY1 genes in these cells. Next, PTOV1 mRNA was knocked-down in prostate cells by lentiviral transduction of two distinct short hairpin RNAs (sh1397 and sh1439). These caused a significant and specific depletion of PTOV1 mRNA and protein levels in RWPE1, in ras-transformed RWPE2 cells, and in PC-3 cells (Figure 1B and Additional file 1: Figure S3) accompanied with a significant upregulation of the endogenous HES1 and HEY1 mRNA levels. Reciprocally, ectopic expression of HA-PTOV1 induced a significant downregulation of endogenous HES1 and HEY1 mRNA and protein (Figure 2A) and inhibited the transactivation of HES1- luciferase by ΔE or ICN, partially and fully activated forms of the Notch1 receptor, respectively, suggesting that PTOV1 acts as a repressor downstream of fully processed Notch1 (Figure 2B) in PC-3, RWPE2 and DU-145 cells. Similar Notch repressor effects by HA-PTOV1 were observed in HeLa and COS-7 fibroblasts transfected with ΔE or ICN, although not in HEK293T cells (Additional file 1: Figure S4).
PTOV1 interacts with the Notch repressor complex at the HEY1 and HES1 promoters
We next analyzed whether the repressive function of PTOV1 on HEY1 and HES1 transcription is associated with its nuclear localization. We have previously described that PTOV1 translocation to the nucleus leads to increased cell proliferation [4, 16]. In the presence of DAPT, endogenous PTOV1 and also SMRT, a component of the Notch repressor complex, showed a markedly increased nuclear localization in PC-3 and LNCaP cells (Additional file 1: Figure S5), suggesting that under conditions of inactive Notch nuclear PTOV1 and SMRT might associate with the Notch repressor complex. As indicated by pull-down assays using extracts of PC-3 cells transfected with FLAG-SMRT, PTOV1 and SMRT interacted with each other (Additional file 1: Figure S5B). Both FLAG-SMRT and endogenous SMRT proteins specifically bound the GST-A and GST-B domains of PTOV1, with the B domain showing a more efficient pull-down.
The association of PTOV1 with the Notch repressor complex was confirmed by co-immunoprecipitation of PTOV1 and FLAG-RBP-Jκ (a Notch-specific DNA binding protein), observed only in the presence of DAPT but not after transfection of constitutively activated Notch (Figure 3A). To corroborate that PTOV1 interacts with the Notch-repressor complex at the HEY1 and HES1 promoters, we used chromatin immunoprecipitation (ChIP). When PC-3 cells were treated with DAPT, ChIP consistently revealed occupation of these promoters by endogenous PTOV1 (Figure 3B and Additional file 1: Figure S6A). RBP-Jκ, but not Notch, was also detected in these conditions. In contrast, when cells were transfected with Notch1-ICN, the HEY1 and HES1 promoters were occupied by ICN and RBP-Jκ, whereas PTOV1 was clearly absent. ChIP with these proteins yielded no amplified bands when using primers for internal HES1 gene sequences and irrelevant immunoglobulins did not pull down DNA associated with these promoters. As an additional control, the co-repressor NCoR was detected at the HEY1 promoter only in the absence of active Notch (Additional file 1: Figure S6B).
Next, the association of PTOV1 with additional elements of the Notch repressor complex was performed by pull-down experiments. In these experiments, full-length GST-PTOV1 interacted with RBP-Jκ, HDAC1, HDAC4 and NCoR (Figure 3C), whereas different components of the Notch repressor complex showed different binding preferences for either PTOV1-A domain or B domain, such that HDAC1 and HDAC4 bound to both PTOV1-A and B domains, while RBP-Jκ and NCoR showed detectable binding only to the PTOV1-A domain or the B domain, respectively (Figure 3C, right panel). These results suggest that, under conditions of inactive Notch, the nuclear localization of endogenous PTOV1 is increased and is associated with several components of the Notch repressor complex at the HEY1 and HES1 promoters. Activated Notch, on the other hand, provokes the dismissal of PTOV1 from these promoters.
PTOV1 repressor activity requires active histone deacetylases
The repressive function of PTOV1 might be linked to the concurrent recruitment to these promoters of co-repressors, such as histone deacetylases (HDACs). To determine this, we treated PC-3 cells with trichostatin A (TSA), an inhibitor of HDACs  that relieves repression at Notch responsive promoters . TSA significantly decreased the repression exerted by HA-PTOV1 on the HES1 promoter, indicating that the PTOV1 repressive function requires active HDACs (Figure 3D). Conversely, transfection of the acetyl transferase CBP, but not p300, enhanced the transactivation of HES1-luciferase promoted by Notch1 and completely abolished the repressive activity of PTOV1 (Figure 3E). Consistently, PTOV1 co-immunoprecipitated with CBP (Figure 3F) , but not with p300. Thus, the repressive action of PTOV1 on the HES1 promoter requires active HDACs, it is enhanced by p300 and is overcome by the expression of CBP.
PTOV1 Suppresses notch function in drosophila melanogaster
To further corroborate the observed functional interactions between PTOV1 and the Notch pathway, we tested the effects of the expression of human PTOV1 on Notch mutant-dependent Drosophila wing patterns. The Notch mutant phenotype was first described in flies, where dosing of Notch produces specific patterns throughout Drosophila development [21, 52, 53]. We generated transgenic flies containing the full-length human PTOV1 cDNA tagged with HA (hPTOV1) under the control of the Upstream Activating Sequence (UAS) promoter to direct the expression of hPTOV1 using the Gal4/UAS system . The expression of hPTOV1 was analyzed using the engrailed-Gal4UAS-GFP line that directs the expression of GFP and hPTOV1 only in the posterior part of the third instar larval wing imaginal discs (Figure 4A). To study the effect of hPTOV1 on patterns associated with loss-of-function of Notch, we used the N55e11 allele, a Notch null mutant that promotes notched wings . When UAS-HA-hPTOV1 was expressed in these heterozygous flies using the nubbin-Gal4 line that drives expression in the central part of the wing disc during larval development, we observed a significant increase in the number of notches per wing (Figure 4B). The Notch gain-of-function phenotype (NAx-M1 allele) results in failure to complete development of the most distal part of vein L5 and in a significant increase of wing size, when cultured at 25°C . Expression of hPTOV1 in the NAx-M1 background restored the L5 vein and the wing size to wild-type patterns, indicating suppression by hPTOV1 of the effects promoted by constitutively active Notch (Figure 4C-D). These results support the conclusion that PTOV1 acts as a negative regulator of the Notch pathway.
PTOV1 is pro-oncogenic in prostate cancer cells
The expression of HA-PTOV1 in PC-3 cells significantly increased invasion compared to control cells and, reciprocally, cells expressing shPTOV1 showed that this protein is required for optimal cell invasion (Figure 5A). Importantly, the gain in invasiveness prompted by overexpression of PTOV1 was abrogated by the concomitant expression of ICN or ΔE. Similarly, knockdown of PTOV1 caused a significant reduction in the ability of PC-3 cells to from spheroids, while expression of HA-PTOV1 stimulated spheroid formation (Figure 5B). On the other hand, constitutive expression of a full-length form of Notch1 in PC-3 cells, that express low endogenous levels of this gene (Additional file 1: Figure S2), caused a significant reduction in their capacity to form spheroids (Figure 5C). These results suggest that PTOV1 promotes, and Notch signaling suppresses, key cellular properties associated with PC progression. The contrasting activities of PTOV1 and HES1 and HEY1 were also tested in HaCaT transformed skin keratinocytes, a cellular model in which Notch has known tumor suppressor functions [31, 55]. In these cells, HA-PTOV1 significantly repressed HES1 and HEY1 expression and promoted cell proliferation and spheroid formation (Additional file 1: Figure S7). Reciprocally, knockdown of PTOV1 in HaCaT cells significantly increased the expression of these genes and decreased spheroid formation, further supporting the notion that high levels of PTOV1 suppress Notch signaling and induce oncogenic properties in different cellular contexts.
PTOV1 is required for tumorigenesis and metastasis of PC-3 prostate cancer cells
We next tested whether PTOV1 is required for the tumorigenic and metastatic properties of PC-3 cells. Cells knocked down for PTOV1 grew significantly smaller (p = 0.0095) subcutaneous tumors in SCID-beige mice compared to control cells transduced with a non-targeting shRNA (Figure 6A). Immunohistochemical analysis of tumors derived from shPTOV1 cells showed strongly increased levels of HES1 and HEY1 proteins as compared to control cells (Figure 6B), consistent with a negative regulation of their expression by PTOV1. In addition, distant metastases of PTOV1 knockdown cells were detected with a significant delay (p = 0.001) as compared to control cells. These results provide evidence that PTOV1 is required for the expression of full tumorigenic and metastatic potentials of PC-3 cells in vivo.
Reciprocal expression patterns of PTOV1 and HEY1 in prostate cancer
To know the relative contributions of PTOV1 and Notch signaling to malignancy in PC, we analyzed the expression of PTOV1, HEY1 and HES1 in 45 prostate adenocarcinomas and control associated benign peripheral zone (BPZ) by real-time RT-PCR. As expected [1, 4], PTOV1 expression was significantly higher in cancer with respect to BPZ (Figure 7A). In contrast, the expression levels of HEY1 were significantly lower in tumors compared to adjacent BPZ, such that a significant inverse correlation was established between the expression levels of HEY1 and PTOV1 (Pearson coefficient = 0.87). The expression levels of a second Notch transcriptional target, HES1, were not significantly altered in tumors compared to BPZ.
Tumor tissues were analyzed at single-cell level by immunohistochemistry for the expression of PTOV1, HEY1 and HES1 proteins on serial sections from 20 primary tumors (n = 10 with Gleason < 7 and n = 10 with Gleason > 7) and 16 lymph node metastases. Epithelial cells from BPZ showed undetectable or faint staining for PTOV1, while a gradual increase in staining intensity was observed from HGPIN lesions to adenocarcinoma lesions, which generally showed a strong staining (Figure 7B-C). In metastases, the staining for PTOV1 was also significantly stronger than in BPZ. In contrast, the expression of HEY1 followed a pattern almost reciprocal to that of PTOV1 and it was significantly stronger in epithelial cells in BPZ and pre-malignant HGPIN compared to cancer and metastasis (Figure 7B-C), confirming the results at the mRNA level. HES1 expression did not show notable differences in intensity between BPZ and tumor areas, although cancerous cells showed a prevalent cytoplasmic localization (Figure 7C). Nevertheless, HES1 expression significantly decreased in metastases (Figure 7B-C), confirming a reciprocal expression pattern between PTOV1 and HES1 in metastatic lesions.
The above results bear not only on any putative roles of PTOV1 in the regulation of HES1 and HEY1 and in prostate cancer progression, but also on the controversial role of Notch in PC [33–39]. Although the results of immunohistochemical analysis show mere correlations between high PTOV1 and low HES1 and HEY1 levels, when taken in the context of the Notch repressor function for PTOV1 described above in cellular models, they are consistent with the notion that high levels of PTOV1 repress the transcriptional activity of Notch in metastatic prostate cancer.
A role for PTOV1 in tumor progression was suggested by previous findings showing its overexpression in PC and other neoplasms in association with increased proliferation rates and higher histological grade [1–6]. Here, we provide evidences suggesting that the pro-oncogenic function of PTOV1 is associated with a downregulation of the Notch target genes HEY1 and HES1. The functional link that we have found between (i) the inhibition of Notch phenotypes in the Drosophila wing, (ii) the upregulation of endogenous HES1 and HEY1 in cells knockdown for PTOV1 and, reciprocally, their inhibition caused by ectopic expression of PTOV1 in PC cells and HaCaT keratinocytes, where Notch acts as tumor suppressor , and (iii) the occupancy by PTOV1 of the HES1 and HEY1 promoters in cells with inactive Notch receptor, provide strong evidences in support of the participation of PTOV1 in the regulation of Notch signaling.
PTOV1 shares similarities with SMRT, a known Notch co-repressor, in the repressive activity on HEY1 and HES1 promoters, the requirement for HDACs and the counteracting effects of histone acetyl transferases [56, 57]. However, while SMRT is excluded from the nucleus by MEKK-1/MEK-1 or IKKα signaling [56, 58], PTOV1 translocates to the nucleus upon stimulation with growth factors [4, 16], and while SMRT is expressed at similar levels in BPZ and PC , PTOV1 is overexpressed in PC. We propose that while SMRT is generally required for the repression of Notch transcriptional activity and other signaling pathways, PTOV1 might be a facultative transcriptional co-repressor with a more restricted scope. Indeed, in response to certain mitogenic signals, PTOV1 translocates to the nucleus, where it could facilitate the transcription of genes necessary for proliferation [4, 16], and invasion (Figure 5A, 5C) while simultaneously repressing Notch targets HEY1 and HES1 genes, as shown in the current study. Reciprocally, Notch activation excludes PTOV1 from these promoters, thus permitting the engagement of Notch-dependent programs while preventing the activation of genes that regulate general proliferation and invasion (Figure 5A, 5C). The function of PTOV1 as a Notch co-repressor could also differ from that of SKIP , since we show here that PTOV1 interacts with the Notch repressor complex, but not with Notch1. Similarly, SHARP, another Notch co-repressor, also interacts with the same inhibitors as PTOV1 [51, 61, 62], but shows different expression patterns in human tumors [6, 61].
The Notch pathway is regulated by positive and negative signals . Although we have shown that PTOV1 can repress the Notch-dependent expression of HEY1 and HES1 in the several cell lines tested, these genes are under the regulation of additional pathways in different cell types or tissues, as suggested by the observation that HES1 expression in Notch1 knockout and in CBF-1/RBP-Jκ knockout mutants is not downregulated [63, 64]. Thus, although HES1 is a bona fide Notch/ RBP-Jκ target, it is also regulated by different signaling cascades in tissues  and in fibroblasts [66, 67].
The evidence presented here suggests that the recruitment of the histone acetyl transferase CBP to the HES1 promoter overcomes the repressive action of PTOV1 on HES1 transcription. In contrast, p300, another major histone acetyl transferase, appears to enhance the transcriptional repression of HES1 by PTOV1. This suggests that these two histone acetyl transferases determine opposing transcriptional states of the HES1 promoter, with CBP favoring a state of active transcription and p300 a state of transcriptional repression. Recent findings indicate that CBP has a stronger trans-activating function than p300 on genes whose products are negative transcription regulators, such as HES1. This is consistent with our observations that PTOV1 and p300 cooperate to repress HES1 transcription, while CBP relieves this repression. Of interest, p300 has been described as a positive inducer of prostate cancer progression, while CBP has been described as a tumor suppressor in the prostate [42, 44, 69, 70]. Together with our observations that PTOV1 expression correlates positively, and HES1 expression negatively, with prostate cancer progression, these evidences may suggest that both PTOV1 and p300, which antagonize Notch target transactivation, function as positive inducers of prostate cancer progression, whereas the Notch signaling and the HES1 activator CBP function as suppressors of prostate cancer establishment and/or progression.
Our evidences also suggest that the function of PTOV1 as a repressor of Notch signaling may have significant consequences for PC progression. Knockdown of PTOV1 in PC-3 cells led to a strong upregulation of HES1 and HEY1 both in vitro and in cells implanted in SCID-beige mice, accompanied with a significant delay in tumor growth and metastatic spread. These pro-oncogenic functions of PTOV1 were also observed in HaCaT keratinocytes, in which Notch behaves as a tumor suppressor . In addition, our evidences suggest that high levels of PTOV1 downregulate HES1 and HEY1 in PC cells by promoting the recruitment of a transcription repressive complex to their promoters. This PTOV1-mediated repression requires active HDACs and is counteracted by the histone acetyl transferase CBP but not p300, suggesting that PTOV1 and Notch activities might be modulated by differential expression of these two enzymes.
In human tissues, we have found evidence of active Notch signaling in the normal prostate epithelium, as attested by the relatively high levels of expression of HES1 and HEY1, as expected [36, 46], while PC metastatic samples expressed significantly lower levels of these proteins, suggestive of a Notch repressed state. PTOV1, on the other hand, showed expression patterns almost reciprocal of those for HEY1 or HES1: low levels or absent in normal epithelium and high levels in metastases. Our observations lend support to a tumor suppressor function of Notch signaling in PC, similarly to its previously demonstrated role in skin, myeloid leukemia and cervical carcinoma cells [30, 55, 71, 72]. Additional evidences are also suggestive of a tumor suppressor function of Notch in PC, including the observations of downregulation of HEY1 and of activated Notch1 [34, 35], and prevention of luminal cell differentiation and induction of proliferation in Notch1 knock-out models [35, 39]. On the other hand, the activation of Notch2 detected in rare metastatic cells , and the overexpression of the Notch ligand Jagged-1 found in metastasis, suggest an oncogenic role for Notch in PC, although no assessment on Notch signaling was done in the same tumors .
Taken together, our observations are compatible with a model whereby PTOV1 contributes to the initiation and progression of PC in part by counteracting the expression of HEY1 and HES1 genes, thus decreasing Notch signaling. These findings are also supportive of a tumor suppressor role of Notch in prostate cancer progression [33–36].
Cell culture, transfection and antibodies
Cell lines were obtained from the American Type Culture collection (Rockville, MD). PC-3, DU-145 and LNCaP prostate cancer cells were maintained in RPMI medium supplemented with 10% heat-inactivated FBS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.1 mM non-essential amino acids (Life Technologies, Grand Island, NY) at 37 ºC in an atmosphere of 5% CO2. COS-7 fibroblasts and HaCaT keratinocytes were maintained in Dulbecco’s modified Eagle’s medium supplemented as above. Human benign prostate-derived epithelial cells RWPE1 and RWPE2  were maintained in Keratinocyte-Serum-Free Medium (Invitrogen, Carlsbad, CA) supplemented with 1 μg/mL human recombinant epidermal growth factor and 10 μg/mL bovine pituitary extract. COS-7 and HeLa cells were transiently transfected for 48 h using Lipofectamine Plus reagents (Invitrogen). Prostate-derived cells were transiently transfected using the Trans IT®-Prostate Transfection Kit (Mirus Bio, Madison, WI). Cells stably transduced by HA-PTOV1 lentivirus or control were selected by flow citometry for GFP-positive cells. PC-3 cell clones c4 and c15, stably expressing Notch1, were obtained by transfection of pcDNA3-Notch1 and selection for two weeks in media containing G418. Control clones, transfected with empty vector, were also selected. DAPT (Sigma-Aldrich, St Louis, MO) was used in cell culture experiments for 4 days at 10 μM. Antibodies to SMRT, HES1 and HEY1 were from Millipore. Antibodies to Notch1 (C-20), GST (1E5) and actin (I19) were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody to γ - secretase-processed Notch1 recognizing Val 1744 was from Cell Signaling. Anti-Flag (M2), Anti-HA and anti β-tubulin were from Sigma-Aldrich.
Full-length human PTOV1 cDNA was obtained from the I.M.A.G.E. Clone Consortium. Constructs harboring the PTOV1 gene were described previously [4, 16]. pIRE-LTXT vector was a generous gift of Dr. Luis Álvarez-Vallina. Partially activated Notch1 ΔE and fully activated intracellular Notch1 (ICN) constructs were kindly provided by Raphael Kopan. Full-length Notch1 in pcDNA3 (FlN1) was a kind gift of Jon Aster.
RNA interference and lentivirus production
Short-hairpin shRNA sequences 1397 and 1439 (Sigma-Aldrich), targeting the human PTOV1 mRNA are shown in Additional file 1: Table S1. Cells transduced with lentiviral particles, were selected with 1 μg/mL puromycin (Sigma-Aldrich) for seven days.
Cells, seeded in 12 well plates, were transfected with test plasmids plus HES1-Luc, or HEY-Luc (0.4 μg), as reporter plasmids and TK-Renilla (0.2 μg) as an internal control for transfection efficiency. The total amount of DNA was kept constant in each experiment by including control pCMV-HA vector. Luciferase assays were performed 48 h after transfection, following the manufacturer’s instructions (Dual Luciferase Reporter Assay Systems, Promega, Madison, WI). Firefly luciferase values were normalized to Renilla values. Each condition was tested in three independent experiments performed in triplicate.
A total of 43 prostate adenocarcinomas from radical prostatectomies performed for T2 to T3 stage tumors were obtained from the archives of the Department of Pathology, Clinic Hospital of Barcelona. The Gleason sum score ranged from 4 to 9 and were stratified as grade < 7 (7 patients) versus > 7 (26 patients). Prostatic tissue from the benign peripheral zone (BPZ) could be evaluated in 10 specimens. Total RNA was prepared from cells or tissues with RNeasy kits (Qiagen, Valencia, CA) and cDNA synthesis was performed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Real-time RT-PCR was performed with the Universal Probe Library system (Roche, Mannheim, Germany) on a LightCycler 480 RealTime PCR instrument (Roche). Specific primers used are shown in Supplementary Information Additional file 1: Table S2. Since the relative amplification efficiencies of target and reference samples were found to be approximately equal, the ΔΔCt method was applied to estimate relative transcript levels. RPS14 amplification levels were used as internal references. Data in triplicates were calculated and presented as mean + SEM.
Immunofluorescence assays of cultured cells were performed as described . DNA was stained with Hoechst 33258. Fluorescent images were captured by confocal microscopy (FV1000, Olympus, Tokyo, Japan) and quantified with Olympus Fluorview software.
Chromatin immunoprecipitation (ChIP)
Chromatin was immunoprecipitated using EZ-chip Chromatin Immuno Precipitation kit (Millipore, Billerica, MA). Briefly, after a mild formaldehyde crosslinking step, cells were sonicated, lysates incubated with primary antibodies and precipitated with protein A/G-Sepharose. Crosslinking of DNA-protein complexes was reversed, DNA purified and used as a template for PCR reactions. Primers used for PCR in ChIP experiments are described in Additional file 1: Table S3.
GST-fusion proteins expressed in Escherichia coli BL-21 strain were purified and stored at -80°C. Pull down assays were performed as described . Protein complexes were analyzed by SDS-PAGE and Western blotting.
Western blotting was performed as described previously . Reactivity was detected with a chemiluminescent substrate (ECL, Amersham Biosciences).
In vitro invasion assays
Assays were performed using growth factor-reduced Matrigel-coated 8-μm pore size Transwell chambers (BD Bioscience, Franklin Lakes, NJ). Invasive cells at the bottom chamber were stained with Hoechst 33258 and scored. Each condition was tested in triplicate.
Spheroid formation assays
Cells (103 cells/well) were plated in triplicate samples in 24-well Ultra Low Attachment plates (Corning) in 1 mL of complete medium containing 0.75% methylcellulose (Sigma-Aldrich) and grown for 14 days before counting.
In vivo tumorigenic assays
The firefly luciferase gene was integrated into the genome of PC-3 cells by lentiviral transduction of a pIRE-LTXT-based construct. shControl and PC-3 shPTOV1 cells (3×106 cells/100 μL PBS:Matrigel (1:1)) were subcutaneously implanted in the rear right flank of 6 week-old male SCID-Beige mice (n = 5 for each cell line) (Charles River Laboratories, Barcelona, Spain). All animal experimental procedures were approved by the Vall d’Hebron Hospital Animal Experimentation Ethic Committee. Tumor growth was monitored twice a week by caliper measurements (D × d2/2, where D is the major diameter and d the minor diameter) and in vivo bioluminescence imaging (BLI). BLI intensity was quantified in photons per second (ph/s) using the IVIS Spectrum Imaging System equipped with the Living Image 4.0 software (Caliper Life Sciences). After reaching 1.5 cm in diameter, mice were anesthetized and primary tumors excised, weighted and imaged by ex vivo BLI. Tumor/control (T/C) weight ratio was calculated by dividing the median value of the tumor weight of the test tumors by the median value of the control group. Mice were monitored for metastatic growth after tumor excision to detect secondary metastases by in vivo bioluminescent imaging using the IVIS Spectrum. Experimental end-point was metastasis detection, after which mice were euthanized and selected tissues analyzed by ex vivo BLI and then processed for histopathology.
Samples from 20 prostate adenocarcinomas, 10 with Gleason < 7 and 10 with Gleason > 7, plus 16 metastases to regional lymph nodes were obtained from the archives of the Department of Pathology, Hospital Vall d’Hebron and the Clinic Hospital of Barcelona. The study was approved by the Institutional Ethical Boards at the Vall d´Hebron Research Institute and the Clinic Hospital. Four μm consecutive or nearly consecutive sections were analyzed by immunohistochemistry with the avidin-biotin peroxidase method. As a negative control, non-specific rabbit antibody was used and gave clean negative results in all cases tested. Positivity was considered when > 10% of the cells showed unequivocal staining. PTOV1, HEY1 and HES1 expression were evaluated in a semiquantitative manner [6, 8], whereby the levels of expression are represented as the percentage of positive cells and the intensity of staining [Hscore = 1 × (% weak) + 2 × (% moderate) + 3 × (% intense) in a range between 0 and 300] [6, 8],
Fly strains and experiments
The Notch alleles N55e11 and NAx-M1 and nubbin-Gal4 and engrailed-Gal4UAS-GFP lines were obtained from the Bloomington Stock Center (Indiana University, Bloomington, IN). The generation of the transgenic lines is described in the Supporting Information. For immunohistochemistry, third instar larval discs were dissected, fixed and processed for staining with specific antibodies. Quantification of wing areas was performed using the NIH ImageJ software.
Results are expressed as means + standard errors of the means. The 2-tailed Student’s t test was used for statistical analysis. A p value < 0.05 was taken as the level of significance. To analyze distributions of qualitative variables, the Pearson coefficient was used. These analyses were performed using the Excel package.
N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester
Intracellular partially activated Notch1
Intracellular activated Notch1
High grade intraepithelial neoplasia
Silencing mediator of retinoid and thyroid hormone receptors/nuclear receptor co-repressor
T cell acute lymphoblastic leukemia
Histone acetyl-transferase CREB-binding protein
Dominant-negative of Mastermind-like gene.
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The authors are grateful to C. Faoro for helping with cell cultures and RNA extractions, E. Castillo and J. Ponsá for helping with the immunoprecipitation and pull-down experiments, and to the Bloomington Stock Center for providing Drosophila stocks.
This work was supported by: Instituto Carlos III RD06/0020/0058 RETICS, Ministry of Science and Innovation SAF2008-03936 and SAF2011-30496 (to R.P.), SAF2008-04136-C02-01 and SAF2011-24686 (to T.M.T.), AGAUR 2009SGR1482 (to R.P. and T.M.T.), Red Nacional de Biobancos, Instituto Carlos III (to R.B.), Fondo de Investigaciones de la Seguridad Social PI20231 (to P.L.F.) and Ministry of Science and Innovation BFU2009-09781 (to F.S.).
The authors declare that they have no competing interest.
Conceived and designed the experiments: R Paciucci. Performed the in vitro culture studies: L Alaña, M Sesé, V Cánovas, C Ruiz. Performed the animal experiments: Y Fernandez, I Abasolo. Supporting technical experiments: Y Puñal, PL Fernandez. Performed the Drosophila experiments: M Sesé, M Corominas, F Serras. Analyzed the data: R Paciucci, L Alaña, M Sesé, I deTorres, T Thomson. Contributed reagents/materials/analysis tools: L Espinosa, A Bigas, S Ramón y Cajal. Wrote the manuscript: R Paciucci, TM Thomson. All authors read and approved the final manuscript.
Lide Alaña, Marta Sesé contributed equally to this work.
Electronic supplementary material
Additional file 1: Table S1: shRNA sequences used for PTOV1 knockdown. Table 2SA. Primers used for real-time RT-PCR using Universal Probe Library (Roche). SB. Primers used for real-time RT-PCR using SYBR Green (Life Technology). Table S3. Primers used for Chromatin inmunoprecipitation (ChIP). Figure S1. The levels of transcription of the Notch target genes HES1 and HEY1 in LNCaP prostate cancer cells are modulated by the γ-secretase inhibitor DAPT. Figure S2. The four different Notch receptors are expressed at variable levels in human prostate cell lines. Figure S3. Western blots illustrating the degree of PTOV1 knockdown in RWPE1, RWPE2 and PC-3 cells by shRNA1397 and shRNA1439. Figure S4. PTOV1 represses Notch dependent HES1 expression in HeLa and COS-7 cells. Figure S5. PTOV1 interacts with the Notch co-repressor SMRT. Figure S6(A) Occupancy by PTOV1 of the endogenous HES1 promoter under inhibition of Notch signaling. S6(B) Occupancy by the co-repressor NCoR of the endogenous HEY1 promoter under inhibition of Notch signaling. Figure S7. PTOV1 promotes proliferation, anchorage-independent growth and repression of Notch targets genes HES1 and HEY1 in HaCaT transformed keratinocytes. (DOCX 2 MB)
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