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Extracellular vesicle microRNAs contribute to Notch signaling pathway in T-cell acute lymphoblastic leukemia
Molecular Cancer volume 21, Article number: 226 (2022)
Abstract
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive T-cell malignancy characterized by genotypically-defined and phenotypically divergent cell populations, governed by adaptive landscapes. Clonal expansions are associated to genetic and epigenetic events, and modulation of external stimuli that affect the hierarchical structure of subclones and support the dynamics of leukemic subsets. Recently, small extracellular vesicles (sEV) such as exosomes were also shown to play a role in leukemia. Here, by coupling miRNome, bulk and single cell transcriptome profiling, we found that T-ALL-secreted sEV contain NOTCH1-dependent microRNAs (EV-miRs), which control oncogenic pathways acting as autocrine stimuli and ultimately promoting the expansion/survival of highly proliferative cell subsets of human T-cell leukemias. Of interest, we found that NOTCH1-dependent EV-miRs mostly comprised members of miR-17-92a cluster and paralogues, which rescued in vitro the proliferation of T-ALL cells blocked by γ-secretase inhibitors (GSI) an regulated a network of genes characterizing patients with relapsed/refractory early T-cell progenitor (ETP) ALLs. All these findings suggest that NOTCH1 dependent EV-miRs may sustain the growth/survival of immunophenotypically defined cell populations, altering the cell heterogeneity and the dynamics of T-cell leukemias in response to conventional therapies.
Background
Acute lymphoblastic leukemia or ALL, is an aggressive malignancy of immature lymphocytes with about 15–20% of cases of T lineage (T-ALL). It is the most common type of cancer in children, but also affects adults with incidences of ~ 30 new cases per 1,000,000 per year [1]. Pediatric T-ALL is largely curable with intensive chemotherapy, but there are significant side effects and ~ 20% of patients suffer relapse. In contrast, adult T-ALL is characterized by a 5-year overall survival of ~ 40% [2].
T-ALL is the result of a malignant alteration of hematopoietic progenitors during T-cell development. A relevant oncogenic pathway involved in T-cell transformation is the NOTCH1 signaling pathway with over 50% of human T-ALL carrying activating mutations of NOTCH1 gene [3, 4].
Recently, small extracellular vesicles (sEV) such as exosomes were reported to contribute to leukemic progression [5, 6]. sEV were shown to reprogram the bone-marrow microenvironment [7], dampen anti-leukemia immune response [8] and promote drug resistance [9]. sEV exert such molecular and cellular functions by transferring molecular information from cancer cells to proximal and/or to distant body districts, including pro-metastatic niche [10]. Importantly, in T-ALL a miRNA-tumor suppressor gene network drives the malignant transformation of T-cell progenitors [11, 12] and cooperates with NOTCH1-driven T-ALL [13,14,15,16,17]. However, the precise role of sEV and miRNA cargo in NOTCH1-driven T-ALL remains elusive. Here, we tackle this issue and present new evidences supporting a central role for EV-miRs in the progression of NOTCH1-driven T-ALL.
Results and discussion
The molecular characteristics of sEV in T-ALL were initially explored in CUTLL1 cell line, a well-characterized human T-cell lymphoma cell line derived from a pleural effusion in a pediatric patient with T-ALL at relapse, with aberrant NOTCH1 activation and strongly sensitive to γ-secretase inhibitors [18]. CUTLL1 cells were lentivirally transduced to constitutively express a dominant-negative form of Mastermind-like protein 1 (dnMAM) to shutdown NOTCH1 signalling, or an empty vector as a control. Indeed, several NOTCH1 target genes were strongly reduced under dnMAM condition (Fig. 1A, B; Fig. S1A). Next, we analyzed size distribution, morphology, quantities of sEV released from CUTLL1-CTRL and CUTLL1-dnMAM cells by nanoparticle-tracking (NTA), TEM, and WB analyses using sEV markers (Fig. 1C-D; Fig. S1B-C). Overall, the prevalent size of sEV matched with expected exosome size distribution (i.e., ~ 30-150 nm; Fig. 1C) and sEV concentration was significantly increased in dnMAM cells (Fig. 1C) in line with previous results showing that a deranged NOTCH signaling in T cells induces a dramatic increase in exosomes release [19]. We detected a total of 318 miRNAs (Fig. 1E; Table S1) by whole-miRNA expression profiling of CUTTL1 cells of which 73 also detected in sEV (i.e., Common-miRs; Fig. 1E; Table S1). Yet, hierarchical clustering analysis showed a set of highly abundant ‘EV-miRs’ comprising members of miR-17-92a cluster and paralogues (Fig. 1F), which we found to be characterized by overrepresented EXOmotifs (Figure S1D), i.e. sorting sequences that determine miRNAs upload into sEV [20, 21]. In line with previous reports, miR-19b is highly expressed in T-ALL cells and is targeted by the t(13;14)(q32;q11) translocation in T-ALL. Likewise, other members of miR-17-92a clusters i.e., miR-20a and miR-92a, were found highly expressed in T-ALL and together with miR-19b were shown being capable of promoting T-ALL [11]. Expression profiling analysis of the Common-miRs in T-ALL cells and in sEV (Fig. 1E), revealed a significant and specific decreased expression of miR-17–92 cluster and paralogues upon NOTCH1 signalling inactivation (i.e., dnMAM vs. CTRL; Fig. 1G) both intracellularly and in sEV (Fig. 1G), which suggests NOTCH1 signalling modulates EV-miRs quantities in sEV. As matter of fact, previous studies showed the existence of a tight interplay between c-MYC and miR-17–92 cluster expression [22] and that c-MYC is an important direct target of Notch-1 in T-ALL [23]. Consistently, we found that miR-17–92 cluster expression is rescued in dnMAM condition upon forced expression of c-MYC (Fig. S1E-G). Next, to investigate the function of these NOTCH1-dependent EV-miRs, we produced PKH26-labelled sEV enriched in miR-17–92 (aka EV_miR-17–92) by overexpressing miR-17–92 cluster in dnMAM cells which yielded sEV enriched in miR-17–92 cluster (Fig. 1H-I; see methods). Internalization of EV_miR-17–92 in dnMAM cells (Fig. 1J) significantly increased the proliferation rate to a comparable level to NOTCH1-proficient CUTLL1 CTRL cells (Fig. 1K). We then treated CUTLL1-wt cells with γ-secretase inhibitor (GSI; see supplemental methods) and observed, as expected, a strong impairment of cell viability (Fig. S2A). Contrariwise, EV_miR-17–92 induced expansion of CUTLL1-wt cells (p < 0.01; Student's T-test; Fig. 2A) and, importantly, were able to rescue the GSI-induced phenotype in T-ALL cells (Fig. 2A). Similar results were obtained by using cells from two independent clones of T-ALL patient-derived xenografts (PDX) (Fig. S2B). Of note, using a known NOTCH1-dependent miRNA i.e. the miR-223-3p (Table S1; [24, 25]) we obtained comparable results in vitro (Fig. S2C-E). Finally, leukemia cells of M71 and H3255 PDX lines were transduced with lentiviruses encoding miR-17–92 cluster or empty vector (EV) as control and subsequently transplanted into immunocompromised (NSG) mice. In line with all previous results, we observed that miR-17–92 transduced human cells indeed rescued the GSI-induced phenotype in T-ALL PDXs (Fig. 2A-B).
Taken together, such results showed, for the first time, the ability of sEV_miR-17–92 to propagate molecular information among T-ALL cells which was able to restore, at least in part, a defective NOTCH1 signalling pathway.
Lastly, we dissected the molecular function of miR-17–92 cluster in the realm of NOTCH1-driven T-ALL. We reasoned that NOTCH1 signalling can be generalized in two main routes: Path-A) the ‘canonical’ transcriptional output of NOTCH1 intracellular domain (NCID) (Fig. 2C); Path-B) the transcriptional output controlled by NOTCH1 through miR-17–92 (Fig. 2C). High-throughput gene expression profiling of CUTLL1 cells ± miR-17–92, and GSI/mock treated (Fig. 2D; see methods) followed by quantitative trait analysis (see methods) identified two transcriptional gene modules, i.e. Mod-A (N = 966 genes) and Mod-B (N = 765 genes), which differ in terms of transcriptional regulation and are both dependent to GSI treatment yet indifferent to rescued miR-17–92 expression (i.e., Mod-A; Fig. 2D; see methods), or reverted (i.e., Mod-B Fig. 2D) (Table S2). Such results confirmed our hypothesis of a bipartite NOTCH1 signalling transcriptional output (Fig. 2C). Notably, MSigDB analysis of Mod-B gene sets revealed a strong and significant enrichment (FDR q-value < 0.0001) of predicted miR-17–92-targeted transcripts (Table S3; see methods) further confirming the regulatory function of the miR-17–92 cluster in Mod-B. Furthermore, IPA software (see methods) revealed that Mod-A comprised canonical NOTCH-signalling genes (e.g., NOTCH2-4, c-MYC, CTNNB1, GATA1-3, etc.) (Fig. S3A) while Mod-B was enriched in gene involved in proliferation (CDKN2A, CCNE1, E2F3, E2F6, RBL1), stemness (FOXM1, TCF4) and cancer (ETS1, RELA, NFE2L2) (Fig. S3B). Intriguingly, when we used Mod-A and Mod-B gene sets to stratify an external cohort of human T-ALL (i.e., the Liu et al. cohort, N = 261; Table S4; [26]), we observed that Mod-B gene set hallmarks T-ALLs particularly enriched in the Early T-cell precursor (ETP) and pre-/post-cortical subtypes, with a higher post-therapeutic minimal residual disease (MRD), and blast count in the bone marrow, that are all characteristics of an adverse outcome [27,28,29](Fig. 2E-F; Table S5).
Next, we performed single-cell RNA sequencing of primary cells, derived from T-ALL patients (N = 2), without any expansion in vivo into immunocompromised mice. Using the Phenograph algorithm [30], we identified several distinct cell subsets (n = 11) (Fig. 2G). Gene set enrichment analysis (GSEA) using Mod-A and -B and hierarchical clustering analysis revealed three main clusters grouping T-ALL cell subsets which shared similar pattern of enrichment scores (ES) (Fig. 2H). In particular, CL2 contains cell subsets contributed by both two patients (Fig. S2F-G) with coherent expression trend of both Mod-A and -B as defined in Fig. 2D, which is a hallmark of activity of NOTCH1 signalling pathway. Indeed, GSEA using Hallmark gene sets (see methods) confirmed that these CL2-cell subsets were significantly characterized by mechanisms involved in proliferation and metabolism (Fig. 2I), which further show how both canonical’ NCID signalling (Mod-A) and NOTCH1 miR-17–92 mediated signalling (Mod-B) can contribute to Notch1-related phenotypes and coexist in the same T-ALL cell subsets.
Our findings shed new light on composite interactions between sEV-miRs, Notch signalling and cellular plasticity that characterize the tumor heterogeneity of T-ALL and promote relapsed/refractory cell subsets of T-cell leukemias. In this scenario, further investigations are needed to explore such mechanisms in T-ALL with the final intent of offering more efficient therapies targeting diverse oncogenic states and microenvironments that support aggressive tumor cells.
Materials and methods
For extensive details on all methodologies see online Supplemental Material and Methods.
Human samples
The institutional ethical committees approved this study (registration number: N91/CE), and informed consent was obtained from all patients enrolled.
Profiling by TaqMan Human MicroRNA Arrays
Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A + B Card Set v3.0 (Applied Biosystems, Foster City, CA).
Genome-wide expression profiling
Gene expression profiling was performed using the GeneChip® Human Clarion S Array (Thermo Fisher Scientific) including more than 210,000 distinct probes representative of > 20,000 well-annotated genes (hg19; Genome Reference Consortium Human Build 37 (GRCh37)).
Single cell RNA-sequencing (scRNA-Seq)
Whole transcriptome analysis at single cell level was performed on FACS-sorted primary T-ALL cells using the BD Rhapsody Single-Cell Analysis System (BD, Biosciences).
Data set availability
The normalized (U6) data for miRNA can be found in Table S1 while mRNA expression data can be accessible at NCBI GEO (GSE193482) and SRA (PRJNA784728 for scRNA-Seq data).
Availability of data and materials
The normalized (U6) data for miRNA can be found in Table S1 while mRNA expression data can be accessible at NCBI GEO (GSE193482; reviewer token: gjubkewovfsvdyr) and SRA (PRJNA784728 for scRNA-Seq data).
Abbreviations
- T-ALL:
-
T-cell acute lymphoblastic leukemia
- sEV:
-
Small extracellular vesicles
- EV-miRs:
-
SEV contain NOTCH1-dependent microRNAs
- GSI:
-
γ-Secretase inhibitors
- ETP:
-
Early T-cell progenitor
- dnMAM:
-
Dominant-negative form of Mastermind-like protein 1
- NTA:
-
Nanoparticle-tracking analysis
- PDX:
-
Patient-derived xenografts
- NCDI:
-
NOTCH1 intracellular domain
- MRD:
-
Minimal residual disease
- GSEA:
-
Gene set enrichment analysis
- IPA:
-
Ingenuity Pathway Analysis
- ddPCR:
-
Droplet Digital PCR
- CTRL:
-
Control
- NES:
-
Normalized enrichment scores
References
Uckun FM, Sensel MG, Sun L, Steinherz PG, Trigg ME, Heerema NA, Sather HN, Reaman GH, Gaynon PS. Biology and treatment of childhood T-lineage acute lymphoblastic leukemia. Blood. 1998;91:735–46.
Pui CH, Evans WE. Treatment of acute lymphoblastic leukemia. N Engl J Med. 2006;354:166–78.
Weng AP, Ferrando AA, Lee W. Morris JPt, Silverman LB, Sanchez-Irizarry C, Blacklow SC, Look AT, Aster JC: Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science. 2004;306:269–71.
Garcia-Peydro M, Fuentes P, Mosquera M, Garcia-Leon MJ, Alcain J, Rodriguez A, Garcia de Miguel P, Menendez P, Weijer K, Spits H, et al. The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model. J Clin Invest. 2018;128:2802–18.
Yang C, Yang H, Liu J, Zhu L, Yu S, Zhang X, Gao L. Focus on exosomes: novel pathogenic components of leukemia. Am J Cancer Res. 2019;9:1815–29.
Kalluri R, LeBleu VS. The biology, function, and biomedical applications of exosomes. Science. 2020;367:eaau6977.
Kumar B, Garcia M, Weng L, Jung X, Murakami JL, Hu X, McDonald T, Lin A, Kumar AR, DiGiusto DL, et al. Acute myeloid leukemia transforms the bone marrow niche into a leukemia-permissive microenvironment through exosome secretion. Leukemia. 2018;32:575–87.
Whiteside TL. Exosomes and tumor-mediated immune suppression. J Clin Invest. 2016;126:1216–23.
Wang J, Hendrix A, Hernot S, Lemaire M, De Bruyne E, Van Valckenborgh E, Lahoutte T, De Wever O, Vanderkerken K, Menu E. Bone marrow stromal cell-derived exosomes as communicators in drug resistance in multiple myeloma cells. Blood. 2014;124:555–66.
Melo SA, Sugimoto H, O’Connell JT, Kato N, Villanueva A, Vidal A, Qiu L, Vitkin E, Perelman LT, Melo CA, et al. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis. Cancer Cell. 2014;26:707–21.
Mavrakis KJ, Van Der Meulen J, Wolfe AL, Liu X, Mets E, Taghon T, Khan AA, Setty M, Rondou P, Vandenberghe P, et al. A cooperative microRNA-tumor suppressor gene network in acute T-cell lymphoblastic leukemia (T-ALL). Nat Genet. 2011;43:673–8.
Ye F. MicroRNA expression and activity in T-cell acute lymphoblastic leukemia. Oncotarget. 2018;9:5445–58.
Mavrakis KJ, Leslie CS, Wendel HG. Cooperative control of tumor suppressor genes by a network of oncogenic microRNAs. Cell Cycle. 2011;10:2845–9.
Li X, Sanda T, Look AT, Novina CD, von Boehmer H. Repression of tumor suppressor miR-451 is essential for NOTCH1-induced oncogenesis in T-ALL. J Exp Med. 2011;208:663–75.
Fragoso R, Mao T, Wang S, Schaffert S, Gong X, Yue S, Luong R, Min H, Yashiro-Ohtani Y, Davis M, et al. Modulating the strength and threshold of NOTCH oncogenic signals by mir-181a-1/b-1. PLoS Genet. 2012;8:e1002855.
Mets E, Van der Meulen J, Van Peer G, Boice M, Mestdagh P, Van de Walle I, Lammens T, Goossens S, De Moerloose B, Benoit Y, et al. MicroRNA-193b-3p acts as a tumor suppressor by targeting the MYB oncogene in T-cell acute lymphoblastic leukemia. Leukemia. 2015;29:798–806.
Mets E, Van Peer G, Van der Meulen J, Boice M, Taghon T, Goossens S, Mestdagh P, Benoit Y, De Moerloose B, Van Roy N, et al. MicroRNA-128-3p is a novel oncomiR targeting PHF6 in T-cell acute lymphoblastic leukemia. Haematologica. 2014;99:1326–33.
Palomero T, Barnes KC, Real PJ, Glade Bender JL, Sulis ML, Murty VV, Colovai AI, Balbin M, Ferrando AA. CUTLL1, a novel human T-cell lymphoma cell line with t(7;9) rearrangement, aberrant NOTCH1 activation and high sensitivity to gamma-secretase inhibitors. Leukemia. 2006;20:1279–87.
Jin K, Wen Z, Wu B, Zhang H, Qiu J, Wang Y, Warrington KJ, Berry GJ, Goronzy JJ, Weyand CM. NOTCH-induced rerouting of endosomal trafficking disables regulatory T cells in vasculitis. J Clin Invest. 2021;131:e136042.
Villarroya-Beltri C, Gutierrez-Vazquez C, Sanchez-Cabo F, Perez-Hernandez D, Vazquez J, Martin-Cofreces N, Martinez-Herrera DJ, Pascual-Montano A, Mittelbrunn M, Sanchez-Madrid F. Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs. Nat Commun. 2013;4:2980.
Garcia-Martin R, Wang G, Brandao BB, Zanotto TM, Shah S, Kumar Patel S, Schilling B, Kahn CR. MicroRNA sequence codes for small extracellular vesicle release and cellular retention. Nature. 2022;601:446–51.
Mihailovich M, Bremang M, Spadotto V, Musiani D, Vitale E, Varano G, Zambelli F, Mancuso FM, Cairns DA, Pavesi G, et al. miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth. Nat Commun. 2015;6:8725.
Weng AP, Millholland JM, Yashiro-Ohtani Y, Arcangeli ML, Lau A, Wai C, Del Bianco C, Rodriguez CG, Sai H, Tobias J, et al. c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma. Genes Dev. 2006;20:2096–109.
Wang R, Yang Z, Liang L, Feng X, Che B, Zhang X, Zheng Q, Yan X, Han H. Notch activation suppresses endothelial cell migration and sprouting via miR-223-3p targeting Fbxw7. In Vitro Cell Dev Biol Anim. 2022;58:124–35.
Ogando J, Tardaguila M, Diaz-Alderete A, Usategui A, Miranda-Ramos V, Martinez-Herrera DJ, de la Fuente L, Garcia-Leon MJ, Moreno MC, Escudero S, et al. Notch-regulated miR-223 targets the aryl hydrocarbon receptor pathway and increases cytokine production in macrophages from rheumatoid arthritis patients. Sci Rep. 2016;6:20223.
Liu Y, Easton J, Shao Y, Maciaszek J, Wang Z, Wilkinson MR, McCastlain K, Edmonson M, Pounds SB, Shi L, et al. The genomic landscape of pediatric and young adult T-lineage acute lymphoblastic leukemia. Nat Genet. 2017;49:1211–8.
Bernt KM, Hunger SP, Neff T. The Functional Role of PRC2 in Early T-cell Precursor Acute Lymphoblastic Leukemia (ETP-ALL) - Mechanisms and Opportunities. Front Pediatr. 2016;4:49.
Anand P, Guillaumet-Adkins A, Dimitrova V, Yun H, Drier Y, Sotudeh N, Rogers A, Ouseph MM, Nair M, Potdar S, et al. Single-cell RNA-seq reveals developmental plasticity with coexisting oncogenic states and immune evasion programs in ETP-ALL. Blood. 2021;137:2463–80.
Berry DA, Zhou S, Higley H, Mukundan L, Fu S, Reaman GH, Wood BL, Kelloff GJ, Jessup JM, Radich JP. Association of Minimal Residual Disease With Clinical Outcome in Pediatric and Adult Acute Lymphoblastic Leukemia: A Meta-analysis. JAMA Oncol. 2017;3:e170580.
Levine JH, Simonds EF, Bendall SC, Davis KL, el Amir AD, Tadmor MD, Litvin O, Fienberg HG, Jager A, Zunder ER, et al. Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis. Cell. 2015;162:184–97.
Acknowledgements
We are grateful to Chiara Di Giorgio for critically editing the manuscript and Dr. Rossella Di Paola for her help in the Illumina next generation DNA sequencing. We would like to thank Dr. Andrew P. Weng (BC Cancer research, Terry Fox Laboratory) for providing us with PDX samples of T-ALL. We also thank Dr. Alessandra Aloisi and Dr. Riccardo Dicorato (CNR-IMM, Lecce Unit) for support with TEM analysis. This study was performed in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of “Casa Sollievo della Sofferenza” Foundation. All authors gave their consent to publication. The gene expression data used in this study are publicly available as indicated in the Methods and Supplementary Information sections.
Funding
This work was in part supported by the Associazione Italiana Ricerca sul Cancro [IG-22827 to F.B.; IG-23070 to V.G.], the Italian Ministry of Health [GR-2016–02363975 and CLEARLY to F.B.; GR-2016–02361287 to V.G; GR-2019–12370460 to T.C.; Ricerca Corrente program 2022–2024 to F.B. and to V.G.], Worldwide Cancer Research [20–0318 to V.G]. T.C. was supported by a fellowship from the Associazione Italiana Ricerca sul Cancro (#19548) and the Umberto Veronesi Foundation. F.T. was supported by a fellowship for Italy from the Associazione Italiana Ricerca sul Cancro (#21010, three-year AIRC fellowship "Pietro Finocchiaro"). R.C. was supported by a fellowship from Fondazione Pezcoller.
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Conception and design: TC, VG, FB; Development of methodology: TC, PP, EDS, FT, VM, RC, GR, VG, FB; Acquisition of data: TC, PP, FT; Analysis and interpretation of data: TC, PP, VG, FB; Writing, review, and/or revision of the manuscript: TC, VG, FB; Administrative, technical, or material support: PP, GR; Study supervision: VG, FB. The author(s) read and approved the final manuscript.
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The institutional ethical committees approved this study (registration number: N91/CE), and informed consent was obtained from all patients enrolled.
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Supplementary Information
Additional file 4.
Supplemental materials and methods.
Additional file 5: Table S1.
miRNA normalized (U6) expression data. Table S2. Mod-A and Mod-B gene sets. Table S3. MSigDB analysis of Mod-B genes. Table S4. Liu et al. T-ALL cohort stratified according to ssGSEA analysis performed with Mod-A and Mod-B gene sets (N=261 patients; see supplemental materials and methods). Table S5. Clinicopathologic characteristics of Liu et al. T-ALL cohort stratified according to ssGSEA analysis performed with Mod-A and Mod-B gene sets (N=261 patients; see supplemental materials and methods).
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Colangelo, T., Panelli, P., Mazzarelli, F. et al. Extracellular vesicle microRNAs contribute to Notch signaling pathway in T-cell acute lymphoblastic leukemia. Mol Cancer 21, 226 (2022). https://doi.org/10.1186/s12943-022-01698-3
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DOI: https://doi.org/10.1186/s12943-022-01698-3