L1CAM protein expression is associated with poor prognosis in non-small cell lung cancer
© Tischler et al; licensee BioMed Central Ltd. 2011
Received: 3 May 2011
Accepted: 10 October 2011
Published: 10 October 2011
The L1 cell adhesion molecule (L1CAM) is potentially involved in epithelial-mesenchymal transition (EMT). EMT marker expression is of prognostic significance in non-small cell lung cancer (NSCLC). The relevance of L1CAM for NSCLC is unclear. We investigated the protein expression of L1CAM in a cohort of NSCLC patients. L1CAM protein expression was correlated with clinico-pathological parameters including survival and markers of epithelial-mesenchymal transition.
L1CAM protein expression was found in 25% of squamous cell carcinomas and 24% of adenocarcinomas and correlated with blood vessel invasion and metastasis (p < 0.05). L1CAM was an independent predictor of survival in a multivariate analysis including pT, pN, and pM category, and tumor differentiation grade. L1CAM expression positively correlated with vimentin, beta-catenin, and slug, but inversely with E-cadherin (all p-values < 0.05). E-cadherin expression was higher in the tumor center than in the tumor periphery, whereas L1CAM and vimentin were expressed at the tumor-stroma interface. In L1CAM-negative A549 cells the L1CAM expression was upregulated and matrigel invasion was increased after stimulation with TGF-beta1. In L1CAM-positive SK-LU-1 and SK-LC-LL cells matrigel invasion was decreased after L1CAM siRNA knockdown.
A subset of NSCLCs with vessel tropism and increased metastasis aberrantly expresses L1CAM. L1CAM is a novel prognostic marker for NSCLCs that is upregulated by EMT induction and appears to be instrumental for enhanced cell invasion.
The 220 kDa transmembrane glycoprotein L1CAM belongs to the neuronal immunoglobulin superfamily of cell adhesion molecules and was first described in neural cell migration  for review see . L1CAM is comprised of six IgG-like domains and five fibronectin-type III repeats, followed by a transmembrane region and a highly conserved cytoplasmic tail [2, 3]. L1CAM protein expression was observed in renal cell cancer, ovarian carcinomas, melanoma, colon cancer, pancreatic cancer, and small cell lung cancer [4–11]. Currently, no data of L1CAM are available for non-small cell lung cancer (NSCLC). Membranous L1CAM enhanced motility of HEK293 cells by interfering with integrin-dependent signaling pathways, inducing endocytosis of beta 1 integrin . L1CAM expression activated extracellular signal-regulated kinase (Erk)-dependent gene regulation and induced NFκB activity conferring increased cell motility and invasion [13, 14].
We previously reported the prognostic importance of epithelial-mesenchymal transition (EMT) for lung cancer . EMT is a cell biological program in which cancer cells lose their epithelial features like E-cadherin expression and up-regulate mesenchymal proteins like vimentin or periostin . Recently, L1CAM has been linked to EMT because protein overexpression decreased the junctional expression of E-cadherin and promoted colony scattering in breast carcinoma cells . As a consequence of EMT, cancer cells acquire a fibroblastic phenotype which enables them to detach from their lattice and to become migratory and invasive. The EMT program is inducible by growth factors like transforming growth factor beta1 (TGF-beta1) or hepatocyte growth factor (HGF) . Several intracellular signaling cascades are involved thereafter, including MAPK and AKT pathways (for review see [17–20]). In pancreatic and endometrial carcinoma cells, L1CAM up-regulation was dependent on TGF-beta1 induction of the EMT transcriptional factor slug [9, 21]. The zinc finger protein slug binds to the E-box motif of the E-cadherin promoter thereby repressing E-cadherin . Further, L1CAM is a target gene of beta-catenin/TCF (T-cell factor) signaling . Topographically, L1CAM was exclusively detected at the invasive front of colorectal cancer and its knockdown reduced haptotactic motility .
The present study aimed to investigate the relationship between L1CAM and indicators of EMT in a large NSCLC patient cohort as well as in NSCLC cell lines. Further, we tested the hypothesis that L1CAM is relevant for patient survival.
Results and Discussion
L1CAM expression in NSCLC
L1CAM expression and clinicopathological parameters
Age ≤64 years
Age > 64 years
Size < 3.7cm
Size ≥ 3.7cm
No vessel infiltration
L1CAM expression is correlated with poor overall and disease free survival
Univariate cumulative survival analysis (54 months)
Multivariate survival analysis
L1CAM expression is correlated with EMT markers
Correlation of L1CAM with EMT markers
L1CAM expression in-vitro
In this study, we demonstrate that L1CAM protein is expressed in NSCLC and is correlated with vessel infiltration, metastasis, and a poor prognosis. In-vivo data suggests that L1CAM is involved in EMT of lung cancer. Further, we provide evidence that L1CAM is involved in NSCLC cell invasion.
L1CAM expression was found in 25% of NSCLC by immunohistochemistry, using our previously described monoclonal anti-L1CAM antibody (clone 14.10), which is directed to the ectodomain of L1CAM (CD171).
Three hundred fifty-three tumors with two L1CAM negative cores were defined as negative. It cannot be ruled out that some of these cases have L1CAM expression due to intratumoral heterogeneity. However, we found L1CAM expression heterogeneity of 13.5%. In these cases, different staining scores were observed in the two cores of the tumor. Of these, 34 tumors had the score "0 and 1" or "1 and 0". These are less than 10% compared to the 353 cases that are L1CAM negative for both cores. In 10 randomly chosen L1CAM negative cases identified by TMA we did not observe focal L1CAM positivity on whole sections. Our finding of L1CAM expression in non-small cell lung cancer is in contrast to a previous protein expression profile study across several human tissues, which was not able to identify L1CAM expression in NSCLC . This discrepancy is most probably due to the low case number (10 SCC/5 ADCA) in that recent expression analysis . Importantly, other studies identified L1CAM expression in small cell lung carcinoma, in pulmonary carcinoids and in large cell lung carcinomas [10, 25]. Interestingly, a relationship to prognosis was also demonstrated in large cell lung carcinomas , corroborating our findings in NSCLC. Only L1CAM was of uni- as well as multivariate prognostic significance for OS and PFS among all biomarkers tested. These data are in line with published prognostic data on L1CAM expression in extrahepatic cholangiocarcinoma, gastric, breast, colorectal, ovarian, endometrial, and pancreatic duct carcinoma [6, 21, 26–30].
We observed a negative correlation of L1CAM with E-cadherin and a positive correlation with slug/vimentin/beta-catenin, indicating a potential role of L1CAM in EMT of NSCLC. Our results are in concordance with findings in colorectal, breast and pancreatic carcinoma where L1CAM expression was clearly involved in the EMT program [9, 16, 23]. It is unclear how L1CAM up-regulation exactly occurs in NSCLC. Several mechanisms have been described so far: In colorectal carcinoma, aberrant L1CAM expression was attributed to a hyperactive beta-catenin/TCF pathway  or to DNA hypomethylation at CpG islands of the L1CAM promoter . In a pancreatic cancer cell line L1CAM expression was regulated via binding of the transcription factor slug to the L1CAM promoter, induced by TGF-beta1 and mediated by c-jun NH2-terminal kinase .
In A549 cells, L1CAM expression was inducible by TGF-beta1 which also induced beta-catenin and vimentin and downregulated E-cadherin. We found the EMT transcription factors slug and snail induced by TGF-beta1 on the mRNA level. Our results would favor a model in which aberrant L1CAM expression in NSCLC is mediated by slug and an activated beta-catenin pathway. A knockdown of slug or snail was not performed so that we cannot prove this hypothesis. L1CAM knockdown in the cell lines SK-LU-1 and SK-LC-LL reduced matrigel invasion but we did not observe further up-regulation of L1CAM expression by TGF-beta1 in the latter cell lines.
Recently, EMT has been linked to the cancer stem cell (CSC) phenotype , and L1CAM was shown to be co-expressed with the CSC marker CD133 in glioma cells . Molecular targeting of L1CAM in CD133+ glioma cells reduced tumor growth and increased survival in-vivo . In a widely accepted hypothesis CSC's are believed to be endowed with increased drug resistance . The existence of bronchoalveolar stem cells (BASC's) and their key role in KRAS-induced lung cancer was proven although it is not assured that BASC's can induce a histophenocopy of the initial tumor in secondary or tertiary hosts (for review see ). It remains to be seen if the dismal prognosis that we observe in the L1CAM positive NSCLC subgroup is related to the presence of CSC's.
We observed a correlation of L1CAM protein up-regulation with blood vessel invasion and metastasis in NSCLC. Further, L1CAM was accentuated at the tumor-stroma interface but decreased in central parts, suggesting an L1CAM involvement in tumor cell invasion. SiRNA knockdown of L1CAM conferred a less invasive phenotype in the two cell lines tested, supporting our in-vivo observation. On whole sections, we found strong L1CAM expression of tumor cells close to and inside intratumoral blood vessels. Up-regulation of L1CAM at the invasive front was also found in colorectal cancer . NSCLC has a highly desmoplastic stroma similar to pancreatic duct carcinoma, often with formation of a central scar. It is unclear whether this prominent fibrotic reaction is inhibiting or rather promoting tumor cell migration. Therefore the question arises whether up-regulation of L1CAM is a prerequisite for infiltrating tumor cells to reach and invade neo-angiogenetic blood vessels. This hypothesis requires further systematic investigation by in-vitro and in-vivo vessel invasion assays. Two recent studies showed that a soluble form of L1CAM acts pro-angiogenic and L1CAM expression in the tumor endothelium mediates selective tumor cell transmigration in pancreatic adenocarcinoma [35, 36].
Anti-L1CAM therapy altered L1CAM gene expression in-vitro as well as reduced tumor growth in a mouse model of intraperitoneally transplanted ovarian cancer cells [37–40]. Thus, anti-L1CAM agents may be used in the setting of intracavitary chemotherapy of malignant effusions. Since protein expression in the carcinoma cells was of similar intensity compared with adjacent peripheral nerves and proximal tubules of the kidney, systemic therapy with mAb to L1CAM could potentially lead to neurological or renal complications.
We demonstrate that the cell adhesion molecule L1CAM is aberrantly expressed in a subset of NSCLC and independently prognostic for poor survival. Further, we show that L1CAM expression is induced by TGFbeta1 and that siRNA knockdown of L1CAM reduces matrigel invasion. The mechanism by which L1CAM leads to a more aggressive tumor phenotype is potentially related to EMT in-vivo and in-vitro. A targeted tumoral anti-L1CAM therapy could support anti-epidermal growth factor receptor or anti-vascular endothelial growth factor inhibition in NSCLC.
Materials and methods
Tumor tissue of 472 consecutive patients with surgically resected primary NSCLC between 1993 and 2002 including 244 squamous cell carcinomas (SCC; 51.7%) and 228 adenocarcinomas (ADCA; 48.3%) was used for construction of a tissue microarray (TMA). Briefly, each patient's tumor was represented by two 0.6mm cores. Histotypes like large cell, adeno-squamous, neuroendocrine or sarcomatoid carcinoma, metastases or relapsing tumors and neo-adjuvantly treated patients were not included. Histotypes were entirely reviewed on H&E and mucin stained sections and controlled by IHC according to recent recommendations . Presence of blood vessel infiltration was identified on H&E and Elastica-Van-Gieson stained whole sections. Progression-free (PFS) and overall (OS) survival data was available for 456 patients. The mean follow-up time was 43 (range 0-169, median 25) and 51 months (range 1-169 months, median 41) for PFS and OS, respectively. The study was approved by the ethical committee of the Kanton of Zurich (reference number StV-29-2009).
Immunohistochemistry and -fluorescence
Immunohistochemistry (IHC) of TMA sections was performed using the following primary antibodies: mAb anti-E-cadherin (Cell Marque Lifescreen Ltd., Rocklin, CA, USA, clone EP700Y, 1:200), mAb anti-beta-catenin (BD Transduction laboratories, Lexington, KY USA, clone 14/beta-catenin, 1:50), mAb anti-vimentin (DAKO A/S, Glostrup, Denmark, 1:250), and mAb anti-slug (Cell Signaling Technology, Inc, Danvers, MA, USA, clone C19G7, 1:100). MAb anti-L1CAM (clone 14.10, directed to the ectodomain, 1:200) was generated as described . Antibodies were tested on a multi tissue TMA for appropriate dilutions. Two protocols were applied: First, on a Ventana Benchmark® platform (Ventana Medical Systems, Tucson, AZ, USA), the CC1 standard pretreatment with 60 min boiling in pH 8 Tris buffer was followed by incubation with primary mAb (E-cadherin, beta-catenin, vimentin) for 60 min at room temperature (RT) and development with the Ultraview-HRP kit, including incubation with respective secondary ab for 30 min at RT. Second, on a Leica Bond® platform (Vision Biosystems, Melbourne, Australia), the H2 standard pre-treatment with 60 min boiling in pH8 Tris buffer was followed by incubation with primary mAb (L1CAM, slug) for 30 min at RT and development with the Refine-DAB Bond kit, including incubation with secondary ab for 30 min at RT and additional polymer amplification. All primary antibodies were diluted in Tris/BSA. To visualize the topographic distribution of protein expression, representative whole sections (n = 5) were stained with double IHC or double IF. For IHC, the Leica Bond® platform with Bond Polymer AP Red Detection and Polymer Refine Detection kits was used. For both systems, hematoxylin counterstain was applied. For IF, sections were boiled for 20 min in citrate buffer and blocked with 5% goat serum for 10 min and incubated overnight with first mAb at 4°C. After washings, slides were probed with an F(ab) goat anti-mouse Alexa-488 (spectrum green, 1:100) or Alexa-546 (spectrum orange, 1:100) for 45 min and counterstained with DAPI. Pictures were taken on an Olympus microscope with CCD camera using the AnalySIS software (Olympus, Munster, Germany).
Intensity of immunoreactivity was semi-quantitatively scored, applying a four-tiered system (0 to 3). L1CAM was independently scored by two pathologists (VT&SH; interobserver kappa-value 0.78). Both scores showed similar results in correlation with EMT markers and clinicopathological parameters. One score (VT) is shown. Peripheral nerves served as internal positive control for L1CAM staining. For L1CAM and E-cadherin membranous staining was counted (score 0 negative, score 1 faint discontinuous, score 2 moderate continuous, score 3 strong continuous). Beta-catenin was separately scored at the plasma membrane, the cytoplasm and the nucleus. Slug was scored in the nucleus. Vimentin was scored in the tumor cell cytoplasm and in peritumoral stromal cells. The two TMA cores were summed up to the sum intensity score (range 0-6).
The lung carcinoma cell lines A549 (ADCA; obtained from ATCC, #CCL-185), SK-LU-1 (ADCA; obtained from ATCC, #HTB-57), H1395 and SK-LC-LL (ADCA and SCC, respectively; obtained from Dr. Reinhard Schwartz-Albiez, DKFZ Heidelberg) were cultivated in RPMI-1640 medium supplemented with 10% fetal bovine serum and 10 mM Glutamine at 37°C, 5% CO2 and 100% humidity. Cells were periodically tested by PCR for mycoplasma. For induction of EMT cells were cultivated in the presence of HGF (Promokine, Heidelberg, Germany) 5 ng/ml or TGF-beta1 (Promokine, Heidelberg, Germany) 10 ng/ml for 7 days.
Quantitative real-time PCR
qRT-PCR was performed as described before . Primers for qRT-PCR were designed using the IDT primer quest programme and were produced by MWG (Ebersberg, Germany). Beta-actin was used as an internal standard. The sequences of primers used are available on request.
SDS-PAGE under non-reducing conditions and transfer of proteins to an Immobilon membrane using semi-dry blotting has been described previously . After blocking with 5% skimmed milk in TBS, the blots were developed with the respective primary antibody as described , followed by peroxidase-conjugated secondary antibody and ECL (Perkin-Elmer, Rodgau, Germany) detection. The mAbs used for Western blot analysis were described before .
For siRNA mediated knockdown, either siGFP or siL1CAM were used. Sequences have been published before . Cells were transfected with Oligofectamine (Invitrogen, Karlsruhe, Germany) 24 h before onset of the Matrigel invasion assay.
Matrigel invasion assay
Tumor cell invasion in-vitro was determined in a double-filter assay. Briefly, Matrigel was layered between two filters, including a lower 5-μm pore nitrocellulose and an upper 8-μm pore polycarbonate filter. The lower filter was fixed by a gel that consisted of human fibrinogen (5 mg/mL) and casein (5 mg/mL) dissolved in serum-containing culture medium. 105 cells were incubated with the filter sandwich for 20 h in 1 mL of medium. The next day, the sandwich was fixed and the filters separated and DAPI stained. Cells attached to the lower filter were counted, and cell invasion was expressed as the ratio of the cell number on the lower filter to the total number of cells in a proliferation control. Five sections per filter were photographed using a Zeiss fluorescence microscope and counted using Image J software.
PFS time was defined as the interval between surgery and disease progression, death or last contact, respectively. Only documented relapses were accepted for warranting progression. Patients with initial metastatic disease were considered to have PFS = 0. OS time was defined as the interval between surgery and death or last contact, respectively. In case of one core loss, data from the remaining core was carried forward. If both cores were not evaluable (4 patients), the case was excluded (n final = 468). Correlations of protein expression intensities with clinico-pathologic parameters were calculated by Kendall's tau b analysis, with PFS and OS by the Kaplan-Meier method using log rank tests. The median value of the sum score was used as cut-off point for dichotomization into a "L1CAM negative" and "L1CAM positive" group. All cases which did not show equal staining intensity for both cores were counted as heterogeneous. Significant univariate prognostic factors were introduced into multivariate analysis applying Cox proportional hazards regression. Statistical analyzes were performed using PASW, version 18.0. P-values < 0.05 were considered significant. Statistical significance of the cell culture experiments was determined by Student's t test. P-values in Figure 4 are indicated as follows: *< 0.05, **< 0.01 ***< 0.001.
We are grateful to Silvia Behnke, Maya Baucamp, Susanne Dettwiler, André Fitsche, Marcel Glönkler, Martina Storz, André Wethmar, and Norbert Wey for excellent technical assistance. This work was supported by a grant from the Fondation Nuovo-Soldati to VT.
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