- Open Access
mRNA modification orchestrates cancer stem cell fate decisions
Molecular Cancer volume 19, Article number: 38 (2020)
Despite their small numbers, cancer stem cells play a central role in driving cancer cell growth, chemotherapeutic resistance, and distal metastasis. Previous studies mainly focused on how DNA or histone modification determines cell fate in cancer. However, it is still largely unknown how RNA modifications orchestrate cancer cell fate decisions. More than 170 distinct RNA modifications have been identified in the RNA world, while only a few RNA base modifications have been found in mRNA. Growing evidence indicates that three mRNA modifications, inosine, 5-methylcytosine, and N6-methyladenosine, are essential for the regulation of spatiotemporal gene expression during cancer stem cell fate transition. Furthermore, transcriptome-wide mapping has found that the aberrant deposition of mRNA modification, which can disrupt the gene regulatory network and lead to uncontrollable cancer cell growth, is widespread across different cancers. In this review, we try to summarize the recent advances of these three mRNA modifications in maintaining the stemness of cancer stem cells and discuss the underlying molecular mechanisms, which will shed light on the development of novel therapeutic approaches for eradicating cancer stem cells.
With the rapid development of high-throughput sequencing technologies, more than 170 types of post-transcriptional RNA modifications have been detected so far . RNA modifications were first identified in non-coding RNA elements like tRNA and rRNA  and have been historically regarded as irreversible decorations on RNA bases. However, subsequent investigations showed that some RNA modifications are actually reversible [3, 4]. Moreover, emerging evidence demonstrates that these dynamic and reversible RNA modifications are widely present in various RNA molecules, not only non-coding RNA but also mRNA. The multitude of RNA modifications led to the birth of “RNA epigenetics” in 2010  and the “Epitranscriptome” in 2012 , which are analogous to the concept of epigenetic modulation mediated by DNA or histone modifications.
Emerging RNA immunoprecipitation-sequencing methods have provided a detailed understanding of the genome-wide landscape of RNA modifications in human cells [7,8,9,10,11,12]. However, the majority of these modifications are mapped to tRNA and rRNA . So far, only a few forms of RNA modifications have been identified in mRNA, such as N6-methyladenosine (m6A), N1-methyladenosine(m1A), Inosine (I), Pseudouridine (Ψ), 5-methylcytosine (m5C), 5-hydroxymethylcytidine (hm5C), N6,2′-O-dimethyladenosine (m6Am), 7-methylguanosine (m7G), and N4-acetylcytidine (ac4C). Despite the low frequency in the human genome, they affect almost every step of mRNA biogenesis and degradation. For example, mRNA modifications extensively modulate a vast pool of biochemical events surrounding mRNA metabolisms, such as mRNA splicing [8, 14], RNA folding [15, 16], stability [17,18,19,20,21], mRNA translation [22,23,24], and RNA transport [25, 26].
Growing evidence indicates that mRNA modifications display dramatic and dynamic variations during lineage commitment and cell reprogramming [27,28,29], suggesting their biological significance in the maintenance of cell identity. As oncogenic transformation frequently accompanies activation of pluripotency genes like NANOG, MYC, and Oct4 [30,31,32], it is likely that mRNA modifications also actively participate in modulating cancer cells’ fate through controlling these oncogenic factors. Consistently, subsequent studies have found that mRNA modifications are also essential for maintaining the stemness and malignancy of cancer stem cells [33,34,35,36].
The concept of cancer stem cells (CSCs) was proposed in the 1970s . Analogous to stem cells in healthy tissue, CSCs possess stem-like properties, including the capacity for self-renewal and the ability to enhanced tumor initiation upon experimental transplantation . It is proposed that the existence of this small but aggressive cell population possesses a high risk of drug resistance and tumor relapse [39, 40]. The CSC hypothesis posits that tumors mirror the hierarchy as normal tissues and that the CSCs are located at the apex of this hierarchical organization [41, 42]. With the elevated capacity of persistent proliferation, CSCs undergo asymmetric division, leading to complicated tumor heterogeneity and resistance to chemotherapy .
A recent breakthrough of the high-throughput sequencing platform has illustrated the detailed epigenetic landscape in CSCs . The epigenetic modifications of DNA or histones are fundamental to the maintenance of cancer stem cell identity [45,46,47,48]. For instance, transformed cells which escape the senescence checkpoint, possess elevated levels of DNA methylation, leading to enhanced self-renewal and pro-survival signals .
However, as a novel modification form in the field of epigenetics, the function of mRNA modification in controlling the stemness of CSCs is still poorly understood. Currently, the three widespread mRNA modification forms are inosine, 5-methylcytosine (m5C), and N6-methyladenosine (m6A). In this review, we will provide an update of how these three mRNA modifications orchestrate regulatory gene networks within CSCs (Table 1). In addition, we will discuss their underlying molecular mechanisms and potential novel therapeutic strategies based on mRNA modification profiles.
A-to-I modification and cancer stem cells
In eukaryotes, adenosine-to-inosine (A-to-I) editing is one of the most prevalent RNA modifications. This process involves hydrolytic deamination of adenosine, catalyzed by the ADAR family members (ADAR1, ADAR2, and ADAR3) . The newly generated inosine base is interpreted by the ribosome as guanosine during mRNA translation, leading to altered protein products, if the modification occurs in the protein-coding region . Among the ADAR family members, ADAR1 and ADAR2 are ubiquitously expressed in eukaryotic cells while ADAR3 is highly expressed in brain cells . Genetic ablation of ADAR1 in mice led to embryonic lethality, at embryonic day E12.5, due to severe global interferon response and defects in hematopoiesis [60, 61]. ADAR2-deficient mice were born at the normal Mendelian ratio and appeared to develop normally, but these mice died within 3 weeks after birth, during or soon after weaning . These results suggest that ADAR1 and ADAR2 are indispensable for embryonic development and normal growth. According to a large-scale study including 6236 patient samples from 17 cancer types, A-to-I modifications display distinct distribution patterns in tumors and normal tissues . However, most modifications existed in the non-coding regions of the mRNA . Despite their prevalence, the functional consequences of these aberrant patterns in tumorigenesis remain elusive.
Previous studies demonstrated that A-to-I modifications could modulate the stemness of hematopoietic malignancies. For example, ectopic expression of ADAR1 potentiated malignant myeloid progenitor expansion through promoting alternative splicing of GSK3β, which enhanced the production of a misspliced form of GSK3β . In vivo studies showed that leukemia progenitor cells harboring this misspliced GSK3β gene displayed enhanced β-catenin expression, which was required for the self-renewal of leukemia stem cells (LSCs) . It was estimated that the genomic amplification of ADAR1 occurred in 30–50% of multiple myeloma patients and portended an unfavorable prognosis . The silencing of ADAR1 attenuated in vivo engraftment of myeloma through suppression of the transcriptional activity of GLI1 , a self-renewal agonist and candidate marker for CSCs . Further studies have shown that ADAR1 can edit exon 12 of the GLI1 transcript, leading to a novel GLI1 protein with a point mutation (Fig. 1a), which might stabilize the GLI1 protein by preventing the binding of a Hedgehog pathway negative regulator . When comparing the A-to-I editing status, scientists revealed the elevated frequency of 3’UTR editing events in malignant progenitor cells. Interestingly, the majority of A-to-I events occurred in the 3’UTR of MDM2 RNA transcripts . As an E3 ubiquitin ligase, MDM2 directly associates with and subsequently inactivates the transactivation domain of tumor suppressor p53. When the A-to-I editing occurred in the 3’UTR of MDM2 transcripts, miR-155 no longer bound to the edited 3’UTR (Fig. 1b), leading to the stabilization of MDM2 and inactivation of p53 .
In addition to mRNA, growing evidence demonstrates that ADAR1 also hinders the biogenesis of tumor-suppressive miRNAs, thereby driving leukemia stem cell self-renewal. Wild-type ADAR1, but not the editing-defective ADAR1E912 mutant, potentiates self-renewal gene expression and suppresses the biogenesis of stem cell inhibitory microRNA let-7 . Subsequent studies found that A-to-G nucleotide changes altered RNA secondary structures at the Drosha/DGCR8 cleavage sites, leading to impaired let-7 miRNA biogenesis (Fig. 1c).
Recent studies have provided substantial new insights into how A-to-I modifications regulate RNA splicing. mRNA maturation involves serial processing steps which structurally alter the newly synthesized RNA transcripts, such as 5′ end capping, RNA splicing, RNA editing, and 3′ end polyadenylation. Among these molecular processes, RNA splicing is a well-documented molecular event that is tightly regulated by A-to-I modification [65, 66]. The creation or removal of splice sites by A-to-I editing plays a vital role in the RNA splicing process. In mammalian cells, A-to-I modifications preferentially occur at Alu elements in the introns of the transcribed gene and create novel splice sites, resulting in exonization of the noncoding sequence. According to high-throughput sequencing results, it was estimated that around 1.4% of total human mRNAs are subject to A-to-I editing and that the editing sites are closely associated with RNA splicing machinery . Another mechanism by which A-to-I editing affects splicing is mediated through altering RNA secondary structures . Because both ADAR proteins and splicing machinery act on double-stranded RNA, the substitution of adenosine by inosine may change the stability of the RNA duplex , and eventually alter the mutual interaction between the splicing machinery and the double-stranded RNAs.
In addition, emerging evidence suggests that A-to-I editing plays a role in regulating RNA stability. In human B cells, DNA and RNA sequencing data showed that the expression levels of thousands of genes were modulated by ADAR proteins . Furthermore, ADAR1 strengthened target RNA stability through physically interacting with HuR, a potent RNA stabilizer. In mouse cells, a similar finding was also reported for ADAR2. The unedited Ctn RNA displayed a higher binding affinity with the RNA destabilizers HuR and PARN when compared to the ADAR2-edited Ctn RNA . However, ADAR2-mediated A-to-I editing of the 3’UTR of Ctn RNA hampered the interaction between the RNA destabilizer and Ctn RNA transcript, thereby leading to a prolonged half-life of Ctn RNA. Although the current findings indicate that both ADAR1 and ADAR2 promote RNA stability through HuR, HuR can function both as an RNA destabilizer or an RNA stabilizer, the mechanism of which is still largely unknown.
In summary, ADAR1 plays a pivotal role in maintaining the stemness of hematopoietic malignancies. Through enhancing self-renewal gene expression and impairing the biogenesis of tumor-suppressive miRNAs, ADAR1 is indispensable for normal hematopoietic stem cell maintenance and leukemia stem cell self-renewal. This suggests that ADAR1 may play an important role in a wide spectrum of hematopoietic disorders which have acquired aberrant stem cell self-renewal features.
m5C modification regulates cancer stem cells
5-methylcytosine (m5C) was first identified in tRNA and rRNA [72, 73]. Recently, the transcriptome-wide landscape of the m5C profile has shown that m5C modifications are preferentially located in the vicinity of the translational start codon of mRNA . In addition, the m5C modification is predominantly catalyzed by the RNA methyltransferase, NSUN2, and the m5C sites are recognized by the m5C reader protein, ALYREF (Fig. 2) . Besides mRNA, NSUN2 also catalyzes tRNA methylation at the variable loop region (C47-C50) . In NSUN2-null mice, the m5C modification was lost in tRNA at the following positions, tRNAGly, tRNALeu, tRNAAsp, and tRNAVal . In addition to NSUN2, DNMT2 is another confirmed m5C RNA methyltransferase [78, 79], which mainly catalyzes tRNA methylation [77, 80].
NSUN2 is highly expressed in various types of solid tumors [81,82,83] and is transcriptionally activated by the MYC oncogene . In bladder cancer, many oncogenic RNAs harbor hyper-methylated m5C sites, which are catalyzed by NSUN2 . YBX1 recognizes m5C-modified mRNA and then recruits mRNA stability maintainer, HuR (Fig. 2), which subsequently stabilizes the putative oncogene HDGF in an m5C-dependent manner .
However, paradoxically, in skin cancer, NSUN2 expression was downregulated and the depletion of NSUN2 increased the population of tumor-initiating cells . By quantifying protein synthesis, it was found that tumor-initiating cells synthesized less protein when compared to their progeny . Thus, a reduction in protein translation is beneficial for the generation of tumor-initiating cells as well as other stem cell types [53, 86].
The above contradictory findings raised two fundamental questions for NSUN2 and m5C methylation in cancer biology: (1) does NSUN2 exert opposite roles in different cancer types, and (2) does m5C methylation either promote or inhibit protein translation based on different microenvironments? It is not rare that a single gene can have dual roles as either a tumor suppressor or an oncogene. For instance, the stem cell marker gene, KLF4, has contrasting roles in various cancer types as reported in previous studies [87, 88]. Because different types of cancer possess distinct contexts and NSUN2 can target multiple RNAs simultaneously, it is likely that NSUN2 exerts its pleiotropic roles in a context-dependent pattern. In other words, if NSUN2 predominantly targets oncogenic RNA molecules in specific types of cancer, it would function as an oncogene. Otherwise, it would act as a tumor suppressor if it mainly affects tumor-suppressive RNA molecules.
Besides, whether the m5C modification promotes or suppresses mRNA translation is still under debate. It was reported that NSUN2-mediated m5C modification can either promote CDK1 and IL-17A translation or attenuate p27KIP1 translation [89,90,91]. In the DNMT2/NSUN2 double knockout cells, overall protein synthesis was dramatically reduced whereas protein translation in single knockout cells was not affected .
Taken together, these contrasting findings indicate that m5C has a sophisticated role in governing mRNA translation and further investigation is necessary to further clarify its mechanism of action.
m5C and the cellular fate of mRNA
Currently, it remains largely unknown how m5C modification alters mRNA expression. Recent findings suggest that m5C might enhance mRNA stability. It was found that YBX1 preferentially recognized mRNA with m5C modifications and subsequently stabilized target mRNAs. In zebrafish early embryos, m5C-modified maternal mRNAs displayed enhanced stability when compared to non-m5C-modified mRNAs . Subsequent mechanistic studies showed that YBX1 enhanced the stability of m5C-modified mRNAs through cooperation with mRNA stabilizer Pabpc1a. This highlights an essential role of m5C modification in RNA metabolism and zebrafish embryo development. In human bladder cancer cells, it was reported that many oncogenic mRNAs were hypermethylated by NSUN2. As an m5C reader, YBX1 recognized m5C sites within HDGF mRNA transcripts and then recruited mRNA stabilizer HuR, leading to enhanced mRNA stability . High expression of oncogenic HDGF mRNA subsequently promoted the pathogenesis of bladder cancer.
Besides, some recent papers indicate that m5C affects not only mRNA stability but also mRNA splicing. It was reported that the distribution of m5C sites partially overlapped with the binding sites of some RNA binding proteins. By analyzing the PAR-CLIP data from public databases, scientists uncovered that m5C sites were enriched in the binding regions of the mRNA splicing factors SRSF3 and SRSF4 , indicating a potent role of m5C modification in modulating mRNA alternative splicing. Interestingly, a recent publication revealed a previously unknown role of m5C modification in HIV infection through modulation of RNA splicing and translation . It was found that HIV-1 RNA transcripts were highly methylated by m5C methyltransferase NSUN2. High-throughput sequencing data subsequently confirmed an m5C site located in the vicinity of the A2 splice site within the Vif gene. Knockout of NSUN2 reduced the use of the D1/A2 splice junction and altered the RNA splicing of HIV RNA transcript. Moreover, loss of NSUN2 reduced m5C occurrence on HIV RNA transcripts and hampered HIV RNA translation, suggesting an important role of m5C modification in the life cycle of HIV. Collectively, these data suggest that m5C modification may be involved in mRNA splicing, although there are many questions that must be addressed by scientists in the coming future. For instance, it is still unclear which reader proteins recognize m5C sites and thereby mediate mRNA splicing. Since only a small number of mRNA splicing factor binding sites overlap with the m5C region, is it a specific phenomenon that occurs in some particular RNA transcripts? Further studies are needed to elucidate the functional role of m5C in alternative splicing.
High-throughput sequencing has provided a detailed mapping of m5C sites in eukaryotic cells. Moreover, the identification of m5C writers and readers has aided our understanding of the functional roles of m5C modifications in the regulation of RNA stability, alternative splicing, and RNA translation. Although scientists have illustrated the genome-wide m5C distribution at single-nucleotide resolution, the role of m5C in mammalian cells remains unclear. For instance, the m5C eraser is still unknown and how m5C mediates other RNA processing steps is to be further explored. Therefore, it is necessary to identify novel m5C-interacting proteins or enzymes, which will further elucidate the functional roles of m5C in various biological events and human diseases.
The dual role of m6A in cancer stem cell
N6-methyladenosine (m6A), occurring at the N6 position of adenosine, is the most pervasive and abundant post-transcriptional modification in eukaryotic cells. By using the antibody-enrichment sequencing method, m6A sites were found in all areas of mRNA transcripts but displayed significant enrichment near the stop codon and 3’UTR region . It was estimated that mRNA transcripts from 7676 mammalian genes have m6A modification . Subsequent studies revealed that 77.29% of m6A sites are present in a consensus motif DRACH (D = A, G or U; R = A or G; H = A, C or U) . Further bioinformatics analysis demonstrates that m6A RNA modification is evolutionarily conserved across different species .
The deposition of m6A in mRNA is mediated by methyltransferase complexes, such as METTL3/14, VIRMA, RBM15/15B, WTAP, HAKAI, and ZC3H13, called ‘writers’. The removal of m6A from mRNA transcript is catalyzed by ‘eraser’ demethylases, FTO and ALKBH5 (Fig. 3a). Owing to the presence of writers and erasers, m6A modification is a dynamic and reversible process that can fine-tune the fate of mRNA transcripts within a short time. This important characteristic allows prompt adaptation to abrupt environmental changes, such as hypoxia and injury.
In the cytoplasm, m6A sites are recognized by m6A binding proteins, such as hnRNPA2B1, YTHDF1/2/3, YTHDC2, and IGF2BP1/2/3, called ‘readers’. Recent advances highlight m6A readers as fundamental players in the modulation of mRNA metabolism (Fig. 3a). It has been reported that the binding of hnRNPA2B1 to m6A sites promotes primary miRNA processing and mediates the nucleocytoplasmic trafficking of mRNAs [98, 99]. YTHDC1 selectively associates with m6A, marks and modulates mRNA alternative splicing, and recruitments of YTHDF1 and YTHDF3 to m6A sites enhance mRNA translation . In addition, some readers also participate in the modulation of mRNA stability. YTHDF2 targets RNA transcripts that contain m6A modifications for degradation , while the binding of IGF2BP1/2/3 to m6A-modified mRNA promotes mRNA stability and translation . In summary, m6A modification tightly modulates most aspects of mRNA processing, including mRNA stability, pre-mRNA splicing, mRNA transportation, and translation.
As the most prevalent post-transcriptional modification, m6A is essential for pluripotency and reprogramming . Transcriptome-wide m6A profiling has shown that the majority of key pluripotent genes (e.g. NANOG, Oct3/4, SOX2, and KLF4) have abundant m6A modifications on their RNA transcripts, which eventually impairs mRNA stability and induces RNA degradation [103, 104]. METTL3 is the core component of the m6A methyltransferase complex. Complete depletion of m6A in METTL3-null mice led to early embryonic lethality owing to prolonged RNA half-life of core pluripotency genes, resulting in a delay in initiation of differentiation programs . Therefore, the correct deposition of m6A in RNA transcripts is essential for the maintenance of self-renewal capacity during embryo development.
Emerging evidence indicates that aberrant m6A profiles frequently occur in a variety of cancer types . Unexpectedly, both elevated and depressed levels of m6A methylation have been reported in different types of cancer, such as liver cancer and acute myeloid leukemia (AML) . In AML, elevated expression of METTL3 has been observed, which led to increased m6A methylation levels of BCL2 and c-MYC transcripts and thus enhanced their translation . METTL3 also induced m6A modification within the mRNA transcript of SP1, an oncogene in AML which modulates c-MYC expression . On the contrary, another group found that m6A demethylase FTO played an oncogenic role in AML through reducing the m6A levels of ABS2 and RARA, which led to decreased mRNA levels of these two targets and eventually contributed to leukemogenesis .
For CSCs, m6A demethylation actively helps to maintain the self-renewal capacity of cancer cells (Fig. 3b). In breast cancer cells, the m6A demethylase, ALKBH5, reduced the level of m6A modification in NANOG mRNA, which subsequently stabilized NANOG mRNA and thus promoted breast cancer stem cell phenotypes . ALKBH5 was also highly expressed in glioblastoma stem-like cells (GSCs) and the knockdown of ALKBH5 attenuated the growth of patient-derived GSCs . The mechanistic study revealed that ALKBH5 demethylated FOXM1 nascent RNA transcripts and enhanced FOXM1 expression, which ultimately maintained the self-renewal capacity of GSCs . Similar to ALKBH5, another m6A demethylase FTO was reported to promote self-renewal and tumorigenesis in GSCs and suppression of FTO by its inhibitor MA2 attenuated GSC growth and self-renewal . Consistently, treatment with another FTO inhibitor R-2HG significantly elevated global m6A modification in leukemia cells, which in turn induced the degradation of MYC/CEBPA RNA transcripts and inhibited the relevant pathways .
However, the opposite expression patterns of m6A exist in acute myeloid leukemia and glioblastoma. METTL14, a core component of the m6A methyltransferase complex, was dramatically elevated in normal hematopoietic stem/progenitor cells and acute myeloid leukemia cells . METTL14 catalyzed the m6A modification in oncogenic factors MYC and MYB, increasing their mRNA stability and thus maintaining the stemness of leukemia stem cells . In glioblastoma, m6A methyltransferase METTL3 was elevated in GSCs and its expression decreased during differentiation . Subsequent studies found that SOX2 mRNA was methylated by METTL3 and that methylated SOX2 mRNA displayed prolonged stability, suggesting that HuR is essential for METTL3-mediated stabilization of SOX2 mRNA .
In summary, aberrant m6A modification frequently occurs in a variety of cancer types and m6A’s deregulation plays a vital role in modulating the stemness of CSCs. However, both elevated and depressed levels of m6A have been reported in CSCs, and the mechanisms by which m6A modification contributes to cell fate decisions remain elusive. Therefore, further studies are needed to explore the underlying molecular mechanisms.
The underlying mechanisms for m6A in RNA expression and splicing
Currently, the mechanisms by which m6A methylation modulates mRNA decay are still under debate. The majority of the current findings indicate that m6A methylation predominantly hampers mRNA stability . It was reported that m6A-modified mRNA has shorter half-live in mammalian cells . Complete depletion of METTL3, the core component of the m6A methyltransferase complex, led to prolonged mRNA half-live when compared to that of wild type cells . On the other hand, knockdown of m6A demethylase, ALKBH5, impaired the stability of NANOG and FOXM1 mRNA transcripts in CSCs [33, 35], indicating that m6A methylation might destabilize mRNA transcripts. Interestingly, recent studies suggest that the destabilizing effect of m6A is attributed to the cytosolic m6A reader protein YTHDF2. The carboxy-terminal of YTHDF2 preferentially binds to m6A-modified mRNAs, and its amino-terminal is responsible for the translocation of the m6A-modified mRNAs towards the P-body, where the unwanted mRNAs are degraded . Furthermore, YTHDF2 silencing results in a prolonged lifetime of its mRNA targets, suggesting that YTHDF2 may play a vital role in mRNA decay .
In contrast to the mRNA-decay-promoting role of m6A methylation, a few emerging studies indicate that m6A methylation also stabilizes mRNA by recruiting IGF2BP1/2/3 and HuR proteins. For example, SOX2 is an m6A target for METTL3 and methylated SOX2 mRNA displays prolonged stability. In addition, RNA stabilizer protein HuR is essential for METTL3-mediated SOX2 mRNA stabilization . On the contrary, IGF2BP1/2/3 proteins can recognize m6A-modified mRNAs and enhance the RNA stability of their target mRNAs in an m6A-dependent manner, thereby modulating cancer cell proliferation .
Therefore, it seems that the cellular fate of m6A-modified mRNA depends on their binding proteins. YTHDF2 recognizes the m6A-modified mRNA transcripts and initiates RNA degradation . However, the binding of IGF2BP1/2/3 or HuR to m6A-modified mRNA enhances mRNA stability and translation [54, 102].
In addition to mRNA decay, the presence of m6A may also participate in mRNA alternative splicing. In 2016, two independent groups reported that m6A sites within the intron affected the splicing of Sxl gene [112, 113], a master regulator of Drosophila sex determination. The m6A mapping results revealed Sxl as a major intronic m6A target and that disruption of the m6A pathway compromised the female-specific Sxl splicing [112, 113]. Further studies demonstrated that m6A reader YT521-B was a dominant m6A effector for female-specific Sxl alternative splicing [112,113,114]. In mammalian cells, a few studies have found that m6A affected RNA splicing by recruiting m6A reader YTHDC1 to m6A-modified mRNA. Mechanistic studies showed that YTHDC1 modulated RNA alternative splicing through interacting with splicing factors [14, 115]. During mouse oocyte development, YTHDC1 regulates m6A-dependent processing of pre-mRNA transcripts through the recruitment of splicing factors CPSF6, SRSF3, and SRSF7. YTHDC1-deficient oocytes displayed extensive alternative polyadenylation, leading to altered 3′-UTR length . In mammalian cells, genome-wide m6A mapping and PAR-CLIP showed that the binding sites of YTHDC1 and SRSF3 co-localized with m6A sites . Subsequent studies found that YTHDC1 promoted exon inclusion by interacting with pre-mRNA splicing regulator SRSF3. To further investigate whether m6A modulates RNA splicing, various high-resolution m6A mapping methods have been used to determine whether m6A sites are located in the vicinity of splice junctions. Some groups have found enrichment of m6A in the proximity of exonic and intronic splice sites [116,117,118], while another independent group found that the majority of m6A sites were not located close to splice sites . These contradicting results raise concerns over the accuracy of current approaches to m6A mapping. More studies will be needed to provide a precise mapping of m6A distribution within the nascent RNAs, which will eventually elucidate the role of m6A in RNA splicing.
As the most prevalent RNA modification form in eukaryotic mRNAs, the m6A-interacting proteins (writers, erasers, and readers) have been identified by serial biochemical approaches. Subsequent studies have highlighted the biological and pathological importance of these proteins. However, the underlying molecular mechanisms of m6A modifications need to be further explored. In conclusion, the central questions remain about how m6A is added on or removed from target mRNAs, and how m6A modulates RNA metabolism.
Conclusion and perspectives
Previous studies highlighted mRNA modifications as key modulators in determining cell fate transition during embryonic development . Recently, emerging evidence demonstrates that several mRNA modification forms are fundamental for maintaining the stemness of CSCs. A unique feature of CSCs is the efficient maintenance of their self-renewal capacity in response to external stimuli such as chemotherapy and radiotherapy. Therefore, depending on the distinct RNA modification profiles between CSCs and other tumor cells, we can exploit this unique feature to develop novel biomarkers to distinguish drug-resistant tumor cells from drug-responsive tumor cells. Furthermore, the dependency on RNA modifications to shift cancer cell fate may be able to be exploited as a powerful therapeutic strategy to specifically eliminate CSCs in cancer patients.
Recent breakthroughs in epitranscriptome sequencing technologies have enabled scientists to decode mRNA modifications in mammalian cells, which strengthen our current understanding of the distribution and function of various mRNA modifications. However, although more than 170 RNA modifications have been identified , only a few sequencing technologies have been established to decode RNA modifications. Moreover, many sequencing platforms fail to provide a precise transcriptome-wide RNA modification landscape at single-base resolution in eukaryotic cells. Thus, more robust and sensitive methods are urgently needed to decipher the epitranscriptome in mammalian cells. Recently, Nanopore technology, a novel single-molecule method, has displayed precise and single base-resolution detection of m6A in synthetic RNA molecules [13, 119]. This single-molecule approach might serve as a novel paradigm to detect different RNA modifications simultaneously.
In addition to novel sequencing strategies, the corresponding RNA modifying enzymes remain largely unknown. For instance, although the m5C methyltransferase NSUN2 has been characterized, we still do not know the parallel demethylases which are responsible for the removal of m5C . Moreover, although the aberrant expression of RNA modifying enzyme has been identified in most aspects of cancer cells, it remains largely unknown how specific RNA modifications affect distinct cancer cell sub-populations. The functional consequences of RNA modification disruption remain unclear. Thus, a detailed understanding of how RNA modifications influence cancer cell fate is essential for harnessing these findings into novel cancer therapies.
In conclusion, the aberrant deposition of RNA modifications is tightly linked to the stemness of CSCs. The underlying molecular mechanisms show that RNA modifications orchestrate almost every step of mRNA metabolism, ranging from mRNA biogenesis to mRNA decay, which can eventually converge to determine the cancer stem cell’s fate and tumor progression.
Availability of data and materials
Alkb homologue 5
Cancer stem cells
- DNMT 1:
DNA methyltransferase 1
DNA methyltransferase 2
Forkhead box protein M1
Antisense to FOXM1
Fat mass and obesity associated protein
Glioblastoma stem-like cells
Human antigen R
IGF2 mRNA binding proteins
Long non-coding RNAs
Leukemia stem cells
Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation
Untranslated terminal region
Wilms tumor 1-associated protein
Y-box binding protein 1
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This work was supported by grants from the National Natural Science Foundation of China (81870449 and 81670601 to Q.Z., 81902886 to W.L.), Special Fund for Frontier and Key Technology Innovation of Guangdong Province (2015B020226004 to Q.Z.), Key Project Fund of Guangdong Natural Science Foundation (2017A030311034 to Q.Z.), Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (Year 2017 to Q.Z.), National Key Point Research and Invention Program of the Thirteenth (2018ZX10723203 to Q.Z.).
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Liang, W., Lin, Z., Du, C. et al. mRNA modification orchestrates cancer stem cell fate decisions. Mol Cancer 19, 38 (2020). https://doi.org/10.1186/s12943-020-01166-w
- RNA modification
- Cancer stem cells
- A-to-I editing