The m6A RNA methylation regulates oncogenic signaling pathways driving cell malignant transformation and carcinogenesis
Molecular Cancer volume 20, Article number: 61 (2021)
The m6A RNA methylation is the most prevalent internal modification in mammalian mRNAs which plays critical biological roles by regulating vital cellular processes. Dysregulations of the m6A modification due to aberrant expression of its regulatory proteins are frequently observed in many pathological conditions, particularly in cancer. Normal cells undergo malignant transformation via activation or modulation of different oncogenic signaling pathways through complex mechanisms. Accumulating evidence showing regulation of oncogenic signaling pathways at the epitranscriptomic level has added an extra layer of the complexity. In particular, recent studies demonstrated that, in many types of cancers various oncogenic signaling pathways are modulated by the m6A modification in the target mRNAs as well as noncoding RNA transcripts. m6A modifications in these RNA molecules control their fate and metabolism by regulating their stability, translation or subcellular localizations. In this review we discussed recent exciting studies on oncogenic signaling pathways that are modulated by the m6A RNA modification and/or their regulators in cancer and provided perspectives for further studies. The regulation of oncogenic signaling pathways by the m6A modification and its regulators also render them as potential druggable targets for the treatment of cancer.
Carcinogenesis is a complex process stemming from genetic and epigenetic changes in a specific cell population. Epigenetic changes that are conventionally known for differential gene expression pattern leading to alternative phenotypic traits involve- altered DNA methylation, modification of histone or remodeling of chromatin structure. Recently, the RNA posttranscriptional modification has emerged as another epigenetic gene expression regulatory mechanism. The ubiquitous presence of modified nucleotides in all kinds of RNA transcripts (both coding and non-coding) has given rise to a completely new concept named ‘Epithanscriptomics’ [1, 2].
Among all the modifications m6A is the most abundant internal modification of mRNAs and long non-coding RNAs (lncRNAs). In mRNAs, m6A sites are highly enriched in 5′-untranslated regions (5′-UTRs), near the stop codons, in the 3′-untranslated regions (3′-UTRs) and within long internal exons. m6A most frequently occurs in the consensus sequence RRACH (R = G/A/U, A = m6A, H=U/A/C) and such sequence occurs about every 85 nucleotides. Thus, the frequency of m6A has been estimated as 3–5 sites per mRNA transcript and 0.1–0.4% of all adenosine nucleotides in mammalian mRNAs [3, 4]. Due to vital functions of m6A modification that are observed in multiple cellular processes, dysregulation of the modification and its regulators have been implicated in various human diseases, especially in different types of cancers as reviewed in recent literatures [3, 5]. Here we discussed recent findings that demonstrate the role of m6A in modulating cellular oncogenic signaling pathways leading to carcinogenesis.
Regulation of m6A modification
The m6A modification is a highly dynamic and reversible process that involves enzymes responsible for- installing the modification called ‘writers’, removal of methylation called ‘erasers’ and recognition of the modification called ‘readers’ (Fig. 1). Members of every classes of regulators work together in a concerted manner to maintain a steady-state balance of m6A level inside the cell.
The m6A writers are methyltransferases that catalyze the transfer of a methyl group from S-adenosylmethionine (SAM) to the N-6 position of adenosine (A). The methyltransferases were first identified as a multicomponent complex in 1994 . Methyltransferase like-3 (METTL3) and its homolog METTL14 were later identified as two components of the complex . Shortly after the discovery of METTL14, Wilms’ tumour 1-associating protein (WTAP) was found to act as another component of methyltransferase complex in mammals . Both METTL3 and METTL14 possess methyltransferase activity and form stable heterodimer while WTAP does not although all of them share the same RNA binding motif. Loss of any of the components markedly decreases the m6A level as well as cell viability . RNA-binding motif protein15 (RBM15) and its paralogue RBM15B were later reported to be parts of the complex facilitating its recruitment to specific sites on RNA molecules . Recently few other components- Vir-like m6A methyltransferase associated protein (VIRMA)/KIAA1429, HAKAI and Zinc finger CCCH domain-containing protein13 (ZC3H13) have been found to interact with other components of the complex thus forming a large multi-component complex. In this complex VIRMA serves as a scaffolding protein holding WTAP, HAKAI and ZC3H13 together to create a binding site for METTL3 and METTL14 favoring their optimal catalytic function . Besides these, an independent m6A writer has been discovered called METTL16, which catalyzes m6A modification in small nuclear RNA, U6 snRNA and other noncoding RNAs (lncRNAs) .
Fat mass and obesity-associated protein (FTO) was the first discovered m6A eraser which regulates cellular energy homeostasis and is associated with obesity. The role of FTO as m6A demethylase further revealed its association with gene expression regulation [12, 13]. ALKBH5 was identified as the second m6A eraser. Both FTO and ALKBH5 belong to Fe2+/α-ketoglutarate dependent dioxygenases enzyme family that are capable of recognizing adenine and cytosine methylation in single stranded DNA and RNA molecules. In mammalian cells a number of ALKB homologs have been identified which include ALKBH1–8, FTO and Tet 1–3, but other than FTO and ALKBH5 none of them showed substrate specificity towards mRNAs or lncRNAs [14, 15]. ALKBH3 has been found as a new m6A demethylase which only exhibits substrate specificity towards tRNAs [16, 17].
While the methylation and demethylation is carried out by writers and erasers, it is the ‘readers’ who determines the functional outcomes of the modification. The YTH domain-containing family proteins1–3 (YTHDF1–3) were discovered as the first group of m6A readers . YTH domain-containing protein1 (YTHDC1) was subsequently identified as a new m6A reader in a study to identify its mode of RNA-binding . The fifth member of the family, YTHDC2 is an RNA helicase which like other family members contains YTH domain, but the feature that distinguishes it from others is the helicase domains that also contribute to RNA binding . m6A-mediated conformational changes called ‘structural switch’ also contribute to exposure of RNA-binding motifs (RBMs) in proteins that are otherwise buried in their native structures giving rise to new group of m6A readers. Alterations in the structures of mRNA and lncRNA by m6A modification were found to facilitate the binding of heterogeneous nuclear ribonucleoprotein C (hnRNPC) which by recognizing m6A facilitates pre-mRNA processing . Another member of this m6A reader family, hnRNPA2B1 plays vital roles in RNA splicing and microRNA (miRNA) processing . The 5′-cap of an mRNA is typically required for protein translation. Eukaryotic initiation factor 3 (eIF3) was identified as an m6A reader, capable of initiating protein translation in a cap-independent manner in the presence of a single 5′-UTR m6A . A distinct class of RNA-binding proteins named insulin-like growth factor-2 mRNA binding proteins 1, 2, and 3 (IGF2BP1–3) were identified as m6A readers by Huang et al. These proteins together with Hu antigen R (HuR) can recognize the m6A modifications in the transcripts, stabilizing them by protecting from degradation .
The subcellular localization of the writers, erasers and readers play crucial roles in determining the fate of m6A-modified transcripts and their biological roles (Table 1). The m6A readers and erasers are co-localized in the nuclear speckles, the primary site for mRNA processing and storage where they exert their methylation-demethylation effects on the target transcripts . The reader proteins have both nuclear and cytoplasmic distribution indicating their functional diversity. The YTH domain containing proteins YTHDF1–3 and YTHDC2 are localized mainly in the cytoplasm while YTHDC1 is found in the nucleus. YTHDF1 recognizes the m6A modification around the stop codon and facilitates mRNA translation. YTHDF2 recognizes the mRNAs which are not destined for translation and accelerates their degradation. YTHDC1 regulates the splicing events by promoting splicing factor SRSF3 or inhibiting SRSF10. It can also interact with the nuclear RNA export factor 1 (NXF1) to facilitate export for mRNA out of the nuclei. YTHDC2 and YTHDF3 can enhance mRNA translation or facilitate mRNA degradation in a context-dependent manner. hnRNPs are localized in the nucleus where hnRNPC can bind and control the processing of the nascent RNA transcripts. hnRNPA2B1 by direct recognition of m6A participates in mRNA splicing and miRNA maturation [16, 25,26,27]. The IGF2BPs (IGF2BP1–3) upon colocalization with HuR in the P-bodies and upon translocation to the stress granules during heat shock, protect mRNA transcripts from degradation. They are also reported to participate in mRNA translation by regulating alternative splicing .
Oncogenic signaling pathways regulated/modulated by m6A methylation
The process of carcinogenesis involves aberrant expressions of key signaling molecules that are tightly regulated by genes encoding them. Given the role of m6A modification in gene expression regulation it is likely to speculate that m6A may contribute to carcinogenesis via up or downregulation of vital components of cellular signaling. Here we compiled the crucial signaling molecules in key signaling pathways that are aberrantly expressed/activated/inactivated in different cancers due to m6A modification and the regulators that act upon them (Fig. 2, Table 2).
The Wnt/β-catenin pathway plays vital roles in developmental processes as well as maintaining adult tissue homeostasis. The key component of this signaling pathway is a glycoprotein Wnt which resides on the surface of the effector cell or is released from it. Wnt binds to its receptor complex on the target cell surface containing Frizzled (Fz) protein and low-density lipoprotein (LDL) receptor-related protein (LRP)5/6 . Inside the cell there are several proteins that participate in transduction of the Wnt mediated signal which include- Dishevelled (Dvl), glycogen synthase kinase-3 (GSK-3), Axin, Adenomatous Polyposis Coli (APC) and β-catenin . In the absence of ligand binding, cytoplasmic β-catenin is constantly degraded by the destruction complex containing Axin, APC, GSK-3 and the serine-threonine kinase CK1. When the Wnt binds to the receptor complex of Fz and LRP5/6, Dvl is recruited which phosphorylates LRP and recruits the Axin complex to the phosphorylated tail of LRP. This disrupts the Axin-scaffolded destruction complex inhibiting the phosphorylation of β-catenin and proteasomal degradation. As a result, the β-catenin is accumulated in the cytoplasm and translocated to the nucleus where it interacts with and activates TCF/Lef1 transcription complex initiating Wnt-activated gene expression [74, 76, 77].
Altered m6A modification and its regulatory protein expression have been observed in different cancer types modulating the Wnt/β-catenin signaling pathway. METTL3 has been found to highly express in hepatoblastoma (HB) which is linked to advanced clinical features like- vascular invasion, distant metastasis or HB recurrence [29, 30]. In molecular level miR-186 have been found to directly target the METTL3 decreasing its expression by directly binding to the 3′-UTR of METTL3 mRNA. In HB tumors the expression of miR-186 was significantly downregulated causing high METTL3 protein expression. This miR-186/METTL3 axis has been found to regulate the Wnt/β-catenin signaling pathway to promote cell proliferation, migration and invasion in HB. With high METTL3 expression and low miR-186 expression, the Wnt/β-catenin pathway-associated proteins such as- β-catenin, APC, cyclin D1 and c-myc were also found overexpressed . METTL3 also regulates the Wnt/β-catenin pathway in HB by increasing the m6A level of important genes in the pathway such as CTNNB1 (β-catenin), CCND1 (cyclin D1), NKD1 (NKD inhibitor of Wnt signaling pathway 1) etc. In HB a significant positive correlation has been found between METTL3 and CTNNB1 gene that encodes for β-catenin. Knockdown of METTL3 downregulated the expression of CTNNB1 expression and stability by decreasing m6A methylation of CTNNB1 transcripts indicating CTNNB1 as a direct downstream target of METTL3 in HB cells .
m6A mRNA methylation has also been reported to play oncogenic roles in PARP inhibitor (PARPi) resistant BRCA-mutated epithelial ovarian cancers (EOC). It was found that FZD10, a receptor and a modulator of Wnt/β-catenin signaling was upregulated due to increased m6A methylation at the 3’UTR region of the FZD10 mRNA. Although the levels of METTL3 and METTL14 did not increase significantly in these cells, the demethylases FTO and ALKBH5 were downregulated which was sufficient to increase m6A level in FZD10 mRNA. Also, the m6A reader IGF2BP2 which plays a role to stabilize m6A-containing mRNAs was upregulated in these cells. Increased m6A in FZD10 mRNA thus increased the β-catenin level in the nuclei thereby upregulating its target genes FOSL1 and CCND1 .
In human osteosarcoma (OS), METTL3 and m6A methylation were found upregulated which activated the oncogenic Wnt/β-catenin signaling. Lymphoid enhancer-binding factor1 (LEF1), an important component of Wnt signaling was the target of METTL3. With METTL3 upregulation, the m6A methylation of LEF1 mRNA was increased which also increased its stability causing Wnt/β-catenin pathway activation . Low ALKBH5 expression was correlated with increased pancreatic adenocarcinoma progression and chemoresistance due to Wnt/β-catenin pathway activation. ALKBH5 overexpression blocked this pathway by m6A demethylation-mediated upregulation of Wnt inhibitory factor-1 (WIF-1) which in pancreatic cancer was overcome by ALKBH5 downregulation . The m6A reader YTHDF1 overexpression was found to positively correlate with the colorectal cancer (CRC) progression via Wnt/β-catenin pathway activation. In CRC cells YTHDF1 recognition of the m6A-modified transcripts of Wnt signaling components FZD9 and WNT6 enhanced their translation. Abnormally increased FZD9 and WNT6 proteins then activated the Wnt/β-catenin pathway to enhance tumorigenicity and CRC stem cell formation . YTHDF1 overexpression has also been correlated with gastric cancer progression via m6A- mediated FZD7 mRNA translation and Wnt/β-catenin hyper-activation .
The PI3K-Akt-mTOR pathway is an interconnected cellular signaling pathway that is crucial in many cellular processes including growth and survival, nutrient uptake, anabolic reactions as well as in pathological conditions, particularly cancers [78, 79]. Phosphatidylinositide 3-kinases (PI3Ks), Akt/protein kinase B (PKB) and mammalian target of rapamycin (mTOR) are the core components of this pathway. PI3Ks are a family of intracellular lipid kinases that phosphorylate the 3′-hydroxyl group of the inositol ring of phosphatidylinositides (PIs) . Upon activation by growth factor receptors, the PI3Ks are recruited to the cell membrane that catalyzes the conversion of phosphatidylinositol-(4,5)-bisphosphate (PI-4,5-P2) to phosphatidylinositol-(3,4,5)-trisphosphate (PI3,4,5-P3). PI3,4,5-P3 or PIP3 acts as a second messenger to initiate the signaling by activating and recruiting the downstream proteins such as PI3K-dependent kinase-1 (PDK1) and Akt. Upon activation Akt can activate/inactivate several other downstream proteins including glycogen synthase kinase 3 (GSK3), IκB kinase (IKK), tuberous sclerosis 2 (TSC2), Bad, caspase 9 and PRAS40 (AKT1S1) etc. By this way Akt can exert a wide range of biological functions including promoting cell proliferation, survival, differentiation, apoptosis, angiogenesis and metabolism . Akt acts as a positive regulator of mammalian target of rapamycin (mTOR) upon activation of vascular endothelial growth factor receptors (VEGFRs). mTOR is a serine/threonine kinase which acts as a catalytic subunit of two distinct protein complexes: mTOR complex1 (mTORC1) and complex2 (mTORC2). Activation of mTORC1 phosphorylates a number of downstream proteins such as S6K1, 4EBP, RPS6K, ULK1 etc. and regulates vital cellular functions like lipid and nucleic acid synthesis, protein synthesis and autophagy thereby regulating cell growth. mTORC2 on the other hand acts on proteins like Akt, PKA, PKC, SGK etc. and regulates cellular functions such as cytoskeletal organization, cell migration, lipid metabolism controlling overall cell survival and proliferation [79,80,81].
Altered expression of the components of PI3K-Akt-mTOR pathway has been reported with alterations in the m6A mRNA methylation or its regulators in different cancer types. In endometrial cancer, a decrease in the m6A mRNA methylation in the mRNA transcripts of the important Akt-mTOR pathway components PHLPP2 (a phosphatase causing Akt dephosphorylation and inactivation) and mTORC2 (which phosphorylates and activates Akt) due to a hotspot R298P mutation in METTL14 and simultaneous downregulation of METTL3 contributed to increased proliferation and tumorigenicity. Decrease in m6A methylation of PHLPP2 mRNA decreased its expression, while decreased m6A methylation in mRNA transcripts of the components of mTORC2 complex (PRR5, PRR5L and mTOR) increased their stability and expression. Increased Akt activation with decreased m6A methylation of the target transcripts due to mutation in METTL14 or METTL3 downregulation contributed to endometrial cancer progression . Similarly, decreased m6A methylation due to decreased METTL3 and increased FTO was also associated with increased tumorignesis in gastric cancer (GC). With METTL3 knockdown the PI3K-Akt pathway was activated as evidenced by Akt and S6 phosphorylation. METTL3 knockdown also activated Wnt/β-catenin pathway as indicated by β-catenin and axin upregulation and GSK-3β phosphorylation. The opposite effect was observed with FTO downregulation. These results indicated a regulator-mediated effect of m6A methylation changes in the target transcripts to be the cause of increased proliferation and invasiveness of gastric cancer cells . Conversely, increased METTL3 expression has been shown to promote ovarian cancer by m6A modification mediated maturation and upregulation of miR-126-5p. In cancer cells miR-126-5p inhibited PTEN expression causing PI3K/Akt/mTOR pathway activation .
Using bioinformatics databases and different gastrointestinal (GI) cancer cell lines Zhao et al. showed that m6A methylation also regulates PI3K-Akt-mTOR pathway in GI cancers by affecting the stability of proteins acting in this pathway. In this study they showed that, m6A methylation in the PTEN, Akt1, PIK3CA and mTOR transcripts decreased their stability and translation. Silencing the METTL3 expression or inhibiting the methyltransferase activity of METTL3-METTL14 writer complex by S-adenosylhomocysteine (SAH) increased their stability and expression but inhibiting the demethylase activity of FTO by meclofenamic acid (MA) had an opposite effect. In addition, the intracellular kinase activity of Akt1, mTOR, GSK3β and p38 was inhibited upon SAH treatment but promoted by MA indicting a modulatory effect of m6A modification on PI3K-Akt-mTOR signaling pathway . In nasopharyngeal carcinoma (NPC), an increased expression of m6A reader YTHDC2 was correlated with the activation of PI3K-Akt/S6 signaling conferring radioresistance to the cancer cells. YTHDC2 was found upregulated in radioresistant NPC cells compared to their radiosensitive counterparts. YTHDC2 activated PI3K-Akt/S6 signaling by increasing the translation of insulin-like growth factor-1 receptor (IGF1R) mRNA . Inverse correlation of METTL3 expression and renal cell carcinoma (RCC) progression and development was reported in another study. In RCC cells, PI3K-AKT-mTOR pathway was activated with decreased METTL3 expression as indicated by increased phosphorylation of the components of this pathway such as p-PI3K, p-AKT, p-mTOR, and p-P70. Upon METTL3 overexpression the levels of these phosphoproteins decreased indicating a suppressive effect of METTL3 on PI3K-AKT-mTOR pathway .
The m6A reader hnRNPA2B1 has been reported to be upregulated in cervical cancer which activated the PI3K-Akt pathway promoting cancer cell proliferation, invasion and migration . The m6A demethylase FTO expression was found to be induced by estrogen in endometrial cancer via activation of PI3K-Akt and MAPK pathway where FTO was the downstream target of PI3K-Akt and MAPK . FTO also activated the PI3K-Akt pathway in breast cancer cells which promoted the glycolysis and lactic acid production . Although m6A methylation changes in any of the mRNA transcripts in the PI3K-Akt pathway was not tested in these studies, it is possible that FTO or hnRNPA2B1 may be involved in regulating their target gene expressions via changing or recognizing their methylation status.
The Janus kinase (JAK)-signal transducer and activator of transcription (JAK-STAT) signaling pathway is associated with cellular proliferation, migration, differentiation and apoptosis aberrant expression of which is linked to different types of cancers . JAK signaling is transduced through the activation of STAT and its target genes which is considered as the canonical mode of signal transduction. However, a non-canonical mode of JAK-STAT signaling was also discovered. In mammalian cells there are four JAK kinase proteins (JAK1–3 and TYK2) which act upon seven STAT proteins (STAT1–4, 5A-B and 6). The cascade of JAK-STAT signal transduction is initiated with the binding of a peptide ligand (such as growth factor or cytokine) to its transmembrane receptor [83, 84]. JAK is associated with the cytoplasmic domains of the receptors in its inactive form. Ligand binding to the receptor causes tyrosine phosphorylation of the cytoplasmic domains of the receptors and causes receptor multimerization which brings two units of JAK kinase in close proximity to each other allowing a transphosphorylation to occur. JAK activation by the receptors in turn causes phosphorylation of the tyrosine residues in the cytoplasmic domains of the receptors which act as docking sites for cytoplasmic STAT proteins. Binding of STAT proteins to the receptor bring them to the JAK kinases which then phosphorylate the tyrosine residues in the C-terminal of STAT proteins and activate them. Upon phosphorylation STATs form homo- or heterodimers and dissociate from the receptor complex translocating to the nucleus. In the nucleus the STAT dimers bind with the promoter regions of their target genes and activate/repress their transcription [83, 85]. The activity of JAK-STAT pathway is negatively regulated by three different class of proteins: suppressors of cytokine signaling (SOCS), protein inhibitors of activated stats (PIAS) and protein tyrosine phosphatases (PTPs). STAT proteins upon activation and translocation in the nucleus activates the transcription of SOCS genes and the resulting SOCS proteins then bind to the JAKs and their associated receptors thus inactivating them. The PIAS proteins on the other hand bind to the STAT dimers and inhibit their binding to the target genes intended for transcription. The PTPs interact with the phosphorylated receptors in the cell membrane or the attached phosphorylated JAKs leading to their dephosphorylation and inactivation [82, 86].
Expression of the components of this pathway may be regulated in the transcriptional level by m6A leading to abnormal signaling in cancer progression. In hepatocellular carcinoma (HCC), METTL3 has been found to upregulate in cancerous cells compared to normal hepatocytes. Knockdown of METTL3 in HCC cells decreased their proliferative and metastatic capability as well as tumorigenicity in nude mice. On the other hand, METTL3 overexpression promoted these effects. SOCS2 has been identified as the target for METTL3-mediated m6A modification which is recognized by YTHDF2 leading to its degradation. Conversely, SOCS2 overexpression due to METTL3 knockdown increased the expression of phosphorylated STAT5 indicating a regulatory role of m6A on the JAK-STAT pathway in HCC progression . Elevated METTL3 level with subsequent downregulation of SOCS2 was also observed in colorectal cancer (CRC). Increased m6A in SOCS2 mRNA decreased its stability contributing to low SOCS2 protein expression in cancerous cells which abolished its inhibitory effect on the leucine-rich repeat-containing G protein-coupled receptor5 (LGR5) expression. LGR5 is responsible for stemness and chemoresistance in colon cancer cells. Thus, increased post-transcriptional m6A modification of SOCS2 promoted cancer cell proliferation via LGR5 induction in CRC . Increased METTL3 expression and decreased SOCS2 protein level was also reported to increase cellular proliferation in gastric cancer cells although no STAT activity could be established in this study .
m6A RNA modification may also contribute to T cell-mediated tumor immunity as METTL3 has been reported to regulate T cell homeostasis and differentiation in knockout animal models. METTL3-mediated m6A modification was found to control the balance between IL-7- mediated JAK-STAT signaling and TCR-mediated ERK-AKT signaling, two important cellular signaling pathways in T cells regulating their homeostasis. Thus m6A accelerates SOCS1, SOCS3 and CISH mRNA degradation activating the IL-7-mediated JAK signaling and the downstream STAT5 activation to re-program the naïve T cell for differentiation and proliferation .
The mitogen-activated protein kinase (MAPK) family proteins are the family of kinases that transmit extracellular signals to the intracellular components which control fundamental cellular processes like growth and differentiation, proliferation, motility, stress response, survival and apoptosis. MAPK pathway converges signals from a wide range of stimuli including stress and DNA damage responses, growth factors, extracellular matrices and responses from cell-cell interaction . Upon receiving signals, the pathway activates the MAPKs in a three-tier kinase activity in which the receptor stimulation first phosphorylates the MAPK kinase kinase (MAPKKK) which in turn phosphorylates MAPK kinase (MAPKK) and MAPK is then phosphorylated and activated by MAPKK. Activation of MAPK pathway act upon diverse cellular components and initiate a series of signaling events. Depending on the type of kinase in the MAPK layer, there are at least four cascades of MAPK pathways: extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK)1/2/3, p38 signaling isoforms (p38 α/β/γ/δ) and ERK5 [89, 90]. The ERK1/2 pathway is the first MAPK cascade discovered and the most well studied of all MAPK pathways. The signaling in ERK pathway is transduced mainly by the Ras-Raf-MEK module where the extracellular signal induce the phosphorylation of small GTPase Ras proteins. Ras in turn recruit and phosphorylates Raf which on its downstream phosphorylates MEK 1 and 2. MEK1/2 then phosphorylates the MAPKs- ERK 1 and 2 and the signal continues by phosphorylation of hundreds of substrates to their downstream in the cytoplasm, nucleus and various other organelles [90, 91]. The JNK cascade was identified as the activator of the transcription factor c-Jun (hence named as JNK) in response to intra and extracellular stresses. The JNK cascade initiates with the activation of small GTPases Rho, Rac and CDC42 which then phosphorylate the MAPKKKs MEKK1/2, MLK1/2, TAK1, ASK1/2, TAO1 etc. Activation of MAPKKKs further phosphorylates the kinases MKK4 and MKK7. Activated MKKs in turn activate the MAPKs JNK1–3 which shuttle between cytoplasm and nucleus and act on their downstream targets such as ATF2, HSF1, STAT3, p53 etc. [91, 92]. Another stress-activated MAPK cascade is p38 pathway which is activated by the same small GTPases and MAPKKK components of JNK cascade but differs from that by the MAPKK components (MKK3 and MKK6) as JNK activators [89, 92]. Unlike other MAPK cascades ERK5 is less studied and hence less understood.
Dysregulated MAPK signaling has been implicated in numerous cancer types. However, the contribution of m6A RNA methylation on regulating this pathway in cancer has started to emerge only in recent days. Decreased METTL14 and increased carcinogenesis in renal cell carcinoma (RCC) has been reported in a recent study. In this study it is shown that, increased m6A modification is negatively correlated with cancer cell migration and invasion through alternative splicing and downregulation of P2RX6, a G-protein coupled Ca2+ ion channel and a preferred receptor for ATP binding. Overexpression of this receptor increases Ca2+ influx in the RCC cells which activates the ERK1/2 pathway. ERK1/2 activation increases the transcription of MMP9 which triggers the invasion and migration of RCC cells. METTL14 downregulation in cancer cells decreases the m6A levels in P2RX6 mRNA, thereby increasing its expression which leads to ERK1/2 MAPK pathway activation promoting RCC cell migration and invasion .
Decreased METTL3 was found correlated with increased tumor size, advancement and metastasis in colorectal cancer (CRC) where METTL3 downregulation activated the MAPK pathway-associated proteins- particularly p38 and ERK resulting in CRC cell proliferation, migration and invasiveness . An opposite effect of METTL3 in CRC was also reported. In this case increased m6A RNA modification was observed in cancerous and surrounding normal tissues due to upregulation of METTL3 which was otherwise low in the normal tissues. METTL3 increased the m6A modification in the pri-miR-1246 promoting its maturation which suppressed the transcription of its downstream target SPRED2 that activates RAF/MEK/ERK pathway. Suppression of SPRED2 inhibits the phosphorylation of RAF/MEK/ERK proteins and abolishes its suppressive effect on migration and invasive capacity of cancer cells. METTL3-mediated m6A modification and upregulation of miR-1246 thus negatively regulate SPRED2 and inactivate MAPK pathway to promote cancer metastasis . METTL3 overexpression contributed to the gastric cancer progression by m6A-mediated downregulation of basic leucine zipper ATF-like transcription factor2 (BATF2). BATF2 acts as a tumor suppressor by enhancing p53 protein stability thereby inhibiting ERK signaling. METTL3-mediated downregulation of BATF2 reversed this effect .
Low YTHDC2 expression was observed in cancerous tissues compared to normal tissues in CRC patients. Bioinformatic analysis showed a positive correlation of YTHDC2 with MAPK pathway and downstream components of intrinsic and extrinsic apoptotic pathways. YTHDC2 activated the p38 MAPK which promoted transcription of a number of genes causing upregulation of exogenous death receptor pathway associated proteins caspase 8, Fas and FasL and the endogenous mitochondrial apoptotic pathway proteins Bax, caspase 9 and cytochrome C . WTAP has been found highly expressed in high-grade serous ovarian carcinoma (HGSOC). Knockdown of WTAP in ovarian cancer cells decreased expression of several MAPK proteins such as p-ERK, ERK, p-JNK, JNK, p38 and p-p38 as well as AKT pathway proteins (p-AKT, AKT) indicating the association of these two pathways with WTAP in ovarian cancer . In hepatocellular carcinoma (HCC), YTHDF2 was downregulated in response to hypoxia leading to HCC cell proliferation. Overexpression of YTHDF2 abolished the hypoxic stress induced MAPK pathway activation by decreasing the phosphorylation of ERK and MEK. YTHDF2 exerted its suppressive effect on MAPK pathway by directly targeting its upstream regulator EGFR recognizing and interacting with m6A on EGFR mRNA thereby promoting its degradation. Thus, YTHDF2 overexpression inhibited the MEK/ERK pathway which was activated by hypoxia and suppresses HCC growth and proliferation .
p53 is a well-known tumor suppressor which is also termed as the ‘guardian of the genome’. It acts as a cellular stress sensor which upon activation by cellular stresses like DNA damage, hypoxia, hyperproliferation, oxidative stress or lack of nutrients triggers feedback responses such as cell cycle arrest, senescence or apoptotic cell death . In a normal cell at its basal state, p53 level is maintained in a steady state level by several negative regulators such as Mdm2, COP1, ARF-BP1 and Pirh2. The p53 regulators are all ubiquitin ligases which bind to p53 to make it a target for degradation by proteasomal pathway . p53 network of proteins are activated by three independent pathways- a) by DNA damage, b) in response to aberrant growth signals and c) chemotherapeutic agents or protein kinase inhibitors. DNA damage by the ionizing radiation recruits the protein kinases called ataxia telangiectasia mutated (ATM) and Chk2 kinases. Activation of ATM is caused by the DNA double-strand break which in turn activates Chk2. Aberrant growth signals triggered by oncogenes like Ras or Myc causes activation of p14ARF protein which activates the p53 network. p53 activation by chemotherapeutic agents or protein kinases are brought about by a group of kinases called ataxia telangiectasia related (ATR) proteins. Activation of these pathways stabilize the p53 protein by inhibiting its degradation resulting in high p53 level inside the cells. Increased p53 level thus induces the transcriptional activation of the downstream signaling proteins to carry out its functions .
Alterations in the m6A status in mRNA transcripts due to changes in m6A regulatory components have been reported to alter the expression of genes that are involved in p53 signaling pathway in various cancer types. Dominissini et al. showed that, knockdown of the METTL3 resulted in changes in more than 20 genes that are associated with p53 signaling pathway. These alterations in p53 pathway proteins which include Mdm2, Mdm4 and p21 are due to alternative mRNA splicing resulting from altered m6A status . In acute myeloid leukemia (AML) patients, coexistence of mutations and/or copy number variations of the m6A regulatory genes and p53 mutations were observed which conferred a poor overall patient survival. However, it is not clear how or to what extent the m6A regulators are contributing to the incorporation of the mutation marks in p53 gene . Copy number variations of m6A regulatory genes and alterations of TP53 status were also observed in clear cell renal cell carcinoma (ccRCC) patients which is associated with poor disease prognosis and overall survival. In these patients the METTL3 and METTL14 transcripts and protein expression were higher than their normal counterparts which are assumed to be significantly associated with TP53 alteration .
In an effort to identify and validate the prognostic signature of the m6A regulators in esophageal carcinoma (ESCA), Yang and colleagues observed several m6A regulatory proteins that were upregulated in ESCA. Among these, high expression phenotype of HNRNPC was associated with activation of cell cycle and p53 signaling pathway . Low expression of m6A eraser ALKBH5 was found in a cohort of pancreatic cancer (PC) patients in which both the mRNA and protein levels of ALKBH5 were downregulated in the cancerous tissues compared to the noncancerous pancreatic tissues. Overexpression of ALKBH5 inhibited the proliferation and tumorigenic potential of the PC cells in both in vitro and in vivo studies. RNA-seq analysis revealed PER1 mRNA, as the most affected transcript which was downregulated upon ALKBH5 loss through elevated m6A and recognition by YTHDF2. Ectopic expression of PER1 in PC cells activated the p53 pathway in an ATM-dependent manner as evidenced by the upregulation of p-ATM, p-CHK2, p-CDC25C, p-p53, p21, and p-CDK1 as well as downregulation of Cyclin B1 indicating a positive regulation of p53 pathway by ALKBH5. Ectopic p53 expression in these cells on the other hand increased ALKBH5 level by direct interaction with its promoter and decreased overall m6A level in the transcripts creating a positive feedback loop. Thus, increased m6A in PC promoted tumor growth and invasiveness due to interruption of the ALKBH5-PER1-p53-ALKBH5 feedback loop .
Increased m6A modification due to METTL3 overexpression was also found to contribute to the mutant p53 expression and increased chemotherapeutic resistance in colon cancer cells. Elevated glycosphingolipid (particularly Gb3) in the glycosphingolipid-enriched microdomains (GEMs) of the cancer cell membrane due to increased glucosylceramide synthase (GCS) activated the cSrc/β-catenin signaling pathway which upregulated the METTL3 expression. METTL3 overexpression increases the m6A modification in the point mutated codon 273 of p53 pre-mRNA with preferential splicing towards mutant protein expression. Suppression of glycosphingolipid Gb3 production or METTL3 silencing suppressed the m6A modification in p53 pre-mRNA and activated the p53 pathway as evidenced by increased p-p53, p21, PUMA and Bax protein expression thereby resensitizing the cells to chemotherapeutic agents [60, 97].
FTO was found upregulated in human non-small cell lung cancer (NSCLC) cells increasing their rate of proliferation. FTO exerted the protumorigenic effects by decreasing the m6A level in the ubiquitin-specific protease-7 (USP7) mRNA thereby stabilizing and increasing USP7 protein expression that played an important role in p53 pathway-dependent tumor progression. Inhibition of USP7 promoted the degradation of E3-ubiquitin ligase Mdm2 inhibiting the ubiquitination and degradation of p53 causing tumor suppression. FTO stabilized the USP7 transcript and caused its upregulation thereby promoting the growth of NSCLC cells by inactivating p53 pathway .
In hepatocellular carcinoma (HCC), METTL3 has been shown to play a protumorigenic role by decreasing the level of RDM1, a key regulator of the DNA double-strand break repair and recombination, RNA processing and protein translation. RDM1 was found to bind with p53 protein prolonging its half-life and modulate the downstream protein p21, Cyclin A1 and 14–3-3σ thus acting as a tumor suppressor. RDM1 was also found to repress the Ras/Raf/ERK signaling pathway in the presence of wild-type p53. METTL3 induced the m6A modification to the RDM1 transcripts which decreased their stability and repressed RDM1 protein expression .
The Hippo signaling pathway is one of the key pathways for controlling organ development or maintaining tissue homeostasis. Hippo pathway maintains it by promoting cell death or inhibiting cellular proliferation or differentiation deregulations of which are also implicated in pathological conditions like cancers . The main components of this pathway are: mammalian Ste20-like kinases 1/2 (MST1/2), large tumor suppressor 1/2 (LATS1/2), Salvador homolog 1 (Sav1), MOB kinase activator 1A and 1B (MOBKL1A and 1B) collectively known as Mob1, Yes association protein (YAP) and TAZ (also known as WWTR1). MST1/2, LATS1/2 and Mob1 form a cascade of kinases which act on YAP and TAZ to phosphorylate and inactivate them . In canonical Hippo signaling, activation of the pathway MST1 and 2 kinases are phosphorylated which further phosphorylate and activate LATS1/2. Activation of LATS1/2 is also carried out by Mob1 kinases. Mob1 is also phosphorylated by MST1/2 which enhances Mob1-LATS1/2 interaction. Sav1 has been shown to act as a scaffolding protein for the Hippo pathway components which can also bind and phosphorylate MST1/2 which in turn phosphorylate Sav1 [98, 99]. LATS1/2 can directly interact with and phosphorylate their downstream target YAP, TAZ and Yki. Phosphorylation of these proteins promotes their retention in the cytoplasm by interaction with the 14–3-3 proteins where they undergo proteasomal degradation. YAP, TAZ and Yki are the downstream effectors of the Hippo pathway, phosphorylation and degradation of which inhibits their nuclear translocation and termination of their activity as transcriptional coactivators [98, 100]. When the Hippo pathway is inactive, YAP, TAZ and Yki proteins translocate to the nucleus where they activate the transcriptional process of their downstream target genes, mainly the TEA-domain-containing (TEAD) family members TEAD1–4, the transcription of which are associated with cell proliferation and death. They also regulate the transcriptional activity of SMAD1/2/3, SMAD7, RUNX1/2, p73 and ErbB4 which are associated with transcription of a wide array of genes involved in cell proliferation, differentiation or developmental processes [98, 99, 101]. With the increasing evidence that YAP and TAZ are highly expressed in solid tumors, these molecules are emerging as crucial determinants of malignant transformations in human cancers [100, 102].
The main determinants of the outcomes of Hippo pathway YAP and TAZ expression have been found to be regulated by m6A modification in human tumors. Increased m6A modification due to increased METTL3 expression and YAP upregulation was observed in human NSCLC cells compared to normal lung cells. Elevated METTL3 level in cancer cells increases m6A modification in YAP mRNA transcripts which are recognized by m6A readers YTHDF1 and YTHDF3 that are also upregulated in NSCLC cells. This subsequently recruits the translation initiation factor eIF3b resulting in an increased YAP level. In addition to this, elevated METTL3 and YTHDF3 also increased the stability and expression of lncRNA MALAT1 which further upregulated the YAP expression via modulation of miR-1914-3p. These all together enhanced tumor growth, metastasis and impart drug resistance to NSCLC cells . In NSCLC, ALKBH5 was found to decrease tumor growth, invasiveness and metastasis by decreasing m6A-mediated YAP expression and inactivation. The m6A reader YTHDF2 recognized the YAP mRNA recruiting argonaute RISC catalytic component 2 (AGO2) to facilitate YAP mRNA degradation. ALKBH5 further decreases the YAP activation by decreasing the miR-107 level in a HuR dependent manner. Thus, reducing m6A methylation of YAP by ALKBH5 contributes to decreased tumor growth and metastasis by YTHDF2-mediated YAP degradation and miR-107/HuR-mediated YAP inactivation .
Elevated METTL3 expression was also found to increase translation of oncoproteins- EGFR and Hippo pathway effector TAZ thereby increasing growth, survival and invasiveness of lung adenocarcinoma (LUAD) cells. METTL3 promoted the translation of target mRNAs by direct recruitment of eIF3 . An opposite effect of m6A methylation in the transcripts was observed in colorectal cancer (CRC) due to YAP1-mediated Hippo pathway activation. In CRC a decreased METTL14 level was observed which resulted in a decreased m6A methylation in the pri-miR-375 inhibiting its maturation by DGCR8. Decreased miR-375 subsequently resulted in upregulation of oncogenes YAP1 and SP1 promoting the tumor progression. In contrast, overexpression of METTL14 inhibited CRC progression by miR-375-mediated downregulation of YAP1 and SP1 .
YTHDF2 was found upregulated in pancreatic cancer which promoted cancer cell proliferation via activation of Akt/GSK3β/Cyclin D1 pathway. However, YTHDF2 overexpression also suppressed the migration and invasive capability of pancreatic cancer cells, an effect termed ‘migration-proliferation dichotomy’. YTHDF2 suppressed the EMT by upregulation and phosphorylation of Mob1 which in turn phosphorylated and activated LATS1 and activated pLATS1 further phosphorylated and inactivated YAP .
Apart from the pathways discussed above, several other cellular signaling pathways such as NFκB, TGFβ, Hedgehog signaling pathway, Notch signaling pathway etc. have also been reported to be either directly or indirectly modified by m6A methylation or its regulatory proteins leading to malignant transformations and cancer development.
NFκB is a regulator of the transcriptional activity of the genes that are involved in inflammation, cell proliferation, survival and malignant transformation. It is shown that, IL-6 in cooperation with lncRNA CUDR induced the malignant transformation of the human embryonic stem cells derived hepatocyte-like stem cells through activation of NFκB signaling by METTL3. Triggered by inflammatory cytokine IL-6, the lncRNA CUDR overexpression enhanced the expression of METTL3 and increased m6A mRNA methylation of the suppressor of variegation 39 homolog-1 (SUV39H1), a histone methyltransferase thereby increasing its expression. SUV39H1 in turn enhanced Histone3 mono-, di- and tri-methylation leading to the expression and phosphorylation of NFκB. Activated p-NFκB translocated to the nucleus and promoted expression and phosphorylation of STAT3. By interacting with the promoter regions of target miRNAs and lncRNAs, p-STAT3 caused the malignant transformation of the hepatocyte-like stem cells . Cytokines released in the tumor microenvironment are also correlated with NFκB activation and tumor progression via changes in cellular m6A status. Ovarian cancer cells co-cultured with macrophages showed higher expression of m6A demethylase ALKBH5 and TLR4. ALKBH5 was also found upregulated in ovarian cancer tissues compared to normal ovarian tissues. It was found that the increased ALKBH5 level was due to TLR4-mediated activation of NFκB pathway. Increased ALKBH5 further targeted the NANOG transcript by increasing m6A level, therefore contributing to increased ovarian cancer stem cell generation and carcinogenesis . Increased METTL3 and m6A-mediated upregulation of estrogen receptor related receptors γ (ERRγ) was shown to trigger chemoresistance in cancer cells. ERRγ interacted with NFκB/p65 to induce transcriptional activation of ABC transporter ABCB1 (thereby increasing expression of P-gp) which facilitated drug efflux . METTL3 has also been found upregulated in bladder cancer (BCa) which promoted proliferation, migration and suppresses the apoptotic cancer cell death. This oncogenic effect of METTL3 is mediated by multiple levels of regulation on MYC oncogene, one of which is activation of NFκB pathway .
METTL3 also exhibited its regulatory effect on Hedgehog pathway promoting prostate cancer cell proliferation, survival and invasive capability. METTL3 exerted these effects by enhancing the expression of GLI1, one of the key Hedgehog signaling proteins which contributed to prostate cancer progression by upregulation of downstream targets c-Myc and cyclin D1 .
In glioma stem-like cells METTL3 was found upregulated which contributed to tumorigenesis via activation of Notch signaling. Several Notch signaling genes (such as notch ligands DLL1, DLL3 and JAG2, notch receptors NOTCH1, NOTCH2 and NOTCH3, and notch target HES1) were found to have enhanced m6A modification which resulted in their upregulation . METTL3 upregulation has also been implicated in promoting migration and invasion potential of liver cancer cells through Snail. METTL3 increased the m6A level in the coding sequence (CDS) of Snail which was recognized by YTHDF1 thus triggering the polysome-mediated translation of Snail mRNA . In HCC cells METTL3 was found SUMOylated by a small ubiquitin-like modifier SUMO1 which increased its expression upon mitogen stimulation. Upregulated METTL3 further regulated the Snail mRNA homeostasis causing HCC progression .
Prospect of m6A targeted cancer therapy
While the role of m6A modification and its regulators in cancer genesis and progression is becoming increasingly evident with increased research efforts in this field in recent years, the question whether this RNA modification can be targeted for cancer therapeutics is yet to be answered. Since no m6A modification-based therapy has been tested so far, it is hard to speculate where we are standing in terms of m6A-targeted therapy development. Some efforts have been made in the way of developing m6A-targeted therapy. Until now most of the efforts to this end are based mainly on the targeting of m6A modifying enzymes. The availability of high resolution crystal structures of these enzymes have facilitated the prediction of potential binding sites which may be utilized to design molecules with high affinity . An in silico method was used to develop ligands which may bind specifically to the m6A methylation writer complex (METTL3/METTL14/WTAP) with high affinity and the ligands were tested for their activity and kinetics of binding toward these enzymes .
The demethylation activity of FTO and ALKBH5 rely on Fe2+, 2-oxoglutarate (2OG) and O2. Inhibitors of 2OG or molecules that interfere with the Fe2+ binding to the demethylases may act as m6A demethylase inhibitors. Niu et al. compiled the list of such compounds that have been tested for their demethylase inhibitor activity, but unfortunately none of the candidate molecules showed selectivity therefore could not be used for therapeutic purposes . The natural compound ‘Rhein’ showed some degree of selectivity with low toxic effect and meclofenamic acid (MA), a non-steroidal anti-inflammatory agent showed potent inhibitory effect against FTO which exhibited promises as demethylase inhibitors. MA2, an ethyl ester derivative of MA is hydrolyzed inside the cell to yield its active form MA. With in vitro experiments MA2 was shown to increase the m6A modification level in HeLa cells and had better cellular penetrability than MA [105, 106]. However, none of these were tested clinically for their effectiveness.
Conclusion and perspectives
m6A regulators may act directly on the key signaling molecules causing their up/downregulation by changing their m6A status or indirectly via acting on and changing the expression levels of other proteins associated with the pathway. For example- METTL3 overexpression contributed to Wnt/β-catenin pathway activation and hepatoblastoma (HB) tumor progression via direct upregulation of Wnt/β-catenin pathway-associated proteins such as- β-catenin, etc. or indirectly via increasing m6A level and upregulation of CTNNB1gene encoding β-catenin [29, 30]. m6A modification has also been shown to simultaneously activate more than one signaling pathways in carcinogenesis. METTL3 has been found to play a protumorigenic role in HCC through suppressing both p53 and Ras/Raf/ERK signaling by acting on a common target that regulates both of these pathways .
While significant progresses have been made in understanding important roles of m6A modification in various oncogenic signaling pathways as discussed in this review, further studies are needed to strengthen the link between m6A modification dysregulation and cancer to establish m6A regulators as effective cancer therapeutic targets. First, m6A modification may play dual roles in cancer, being oncogenic in some cases while tumor suppressive in others. In human osteosarcoma (OS), upregulation of METTL3 increased tumor progression by activating Wnt/β-catenin pathway while in renal cell carcinoma (RCC), lower METTL3 expression was associated with increased tumor size and advancement due to PI3K-AKT-mTOR pathway activation [32, 42]. Why the same molecule plays opposite roles needs further insight. This seemingly contrasting phenomena was also observed in similar cancer types as in the case of colorectal cancer (CRC). In one instance, increased METTL3 expression was correlated with decreased tumor size, advancement and metastasis, while in the other METTL3 was found to promote CRC metastasis. One explanation for these discrepant results may be the variability in the distribution of patient populations based on METTL3 expression. In the latter case the populations were evenly distributed between high and low expression groups, while in the former case there was a high variability between these two groups which may have contributed to the variable outcome of the analysis. These discrepancies may also be due to differences in signaling molecules targeted by METTL3, either activating or suppressing the MAPK pathway [51, 52]. Similar discrepancy was also observed in case of gastric cancer (GC) where METTL3 downregulation was correlated with malignant phenotype in one case while the opposite was observed in the other. Again, this may be due to variable population size or differences in the molecules targeted by METTL3 thereby activating two distinct pathways [36, 54]. Second, current studies on the role of m6A modification in cancer are mostly carried out in cancer cells, whether m6A modification dysregulation plays a role in the process of converting normal cells to cancer cells remains largely unknown . Further studies are needed to determine whether and how m6A modification dysregulation contributes to cell malignant transformation caused by various cancer etiology factors. Third, it is now known that m6A modifications are present in different types of cellular RNAs (e.g. mRNAs, lncRNAs, miRNAs etc.). However, current studies on the role of m6A in modulating oncogenic signaling pathways focus mainly on mRNAs. Further studies are needed to determine the role of m6A dysregulation in non-coding RNAs especially the lncRNAs in regulating oncogenic signaling pathways in cancer initiation and progression. Fourth, m6A modification affects signaling molecules in more than one pathway simultaneously, it is highly likely that altered expression of a single m6A regulator may play key roles in dysregulating multiple oncogenic pathways. Therefore, targeting a single m6A regulatory molecule may lead to inhibition of multiple oncogenic signaling pathways (Fig. 3). Further studies are needed to determine whether targeting m6A regulators alone or in combination with other currently approved anticancer drugs could be effective cancer therapeutic strategies.
Availability of data and materials
5′ untranslated regions
3′ untranslated regions
Wilms’ tumour 1-associating protein
RNA-binding motif protein
Vir-like m6A methyltransferase associated protein
Zinc finger CCCH domain-containing protein13
Fat mass and obesity-associated protein
YTH domain-containing family proteins
Long non-coding RNA
Heterogeneous nuclear ribonucleoprotein C
Eukaryotic initiation factor 3
Insulin-like growth factor-2 mRNA binding proteins
Hu antigen R
Nuclear RNA export factor 1
Low-density lipoprotein (LDL) receptor-related protein
Glycogen synthase kinase-3
Adenomatous Polyposis Coli
Epithelial ovarian cancer
Lymphoid enhancer-binding factor 1
Wnt inhibitory factor1
Protein kinase B
Mammalian target of rapamycin
Glycogen synthase kinase 3
Tuberous sclerosis 2
Vascular endothelial growth factor receptors
Insulin-like growth factor 1 receptor
Renal cell carcinoma
Signal transducer and activator of transcription
Suppressors of cytokine signaling
Protein inhibitors of activated stats
Protein tyrosine phosphatases
Leucine-rich repeat-containing G protein-coupled receptor 5
Mitogen-activated protein kinase
MAPK kinase kinase
Extracellular signal-regulated kinase
c-Jun N-terminal kinase
Basic leucine zipper ATF-like transcription factor 2
High-grade serous ovarian carcinoma
Ataxia telangiectasia mutated
Ataxia telangiectasia related
Acute myeloid leukemia
Clear cell renal cell carcinoma
Non-small cell lung cancer
Mammalian Ste20-like kinases 1/2
Large tumor suppressor 1/2
Salvador homolog 1
MOB kinase activator
Yes association protein
Argonaute RISC catalytic component 2
Suppressor of variegation 39 homolog 1
Estrogen receptor related receptors γ
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This work was supported by the National Institutes of Health [R01ES026151; R01ES028256; R01ES029496; R01ES029942].
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Uddin, M.B., Wang, Z. & Yang, C. The m6A RNA methylation regulates oncogenic signaling pathways driving cell malignant transformation and carcinogenesis. Mol Cancer 20, 61 (2021). https://doi.org/10.1186/s12943-021-01356-0