LIM only 4 is overexpressed in late stage pancreas cancer
© Yu et al; licensee BioMed Central Ltd. 2008
Received: 04 August 2008
Accepted: 22 December 2008
Published: 22 December 2008
LIM-only 4 (LMO4), a member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors, was initially reported to have an oncogenic role in breast cancer. We hypothesized that LMO4 may be related to pancreatic carcinogenesis as it is in breast carcinogenesis. If so, this could result in a better understanding of tumorigenesis in pancreatic cancer.
We measured LMO4 mRNA levels in cultured cells, pancreatic bulk tissues and microdissected target cells (normal ductal cells; pancreatic intraepithelial neoplasia-1B [PanIN-1B] cells; PanIN-2 cells; invasive ductal carcinoma [IDC] cells; intraductal papillary-mucinous adenoma [IPMA] cells; IPM borderline [IPMB] cells; and invasive and non-invasive IPM carcinoma [IPMC]) by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR).
9 of 14 pancreatic cancer cell lines expressed higher levels of LMO4 mRNA than did the human pancreatic ductal epithelial cell line (HPDE). In bulk tissue samples, expression of LMO4 was higher in pancreatic carcinoma than in intraductal papillary-mucinous neoplasm (IPMN) or non-neoplastic pancreas (p < 0.0001 for both). We carried out microdissection-based analyses. IDC cells expressed significantly higher levels of LMO4 than did normal ductal epithelia or PanIN-1B cells (p < 0.001 for both) or PanIN-2 cells (p = 0.014). IPMC cells expressed significantly higher levels of LMO4 than did normal ductal epithelia (p < 0.001), IPMA (p < 0.001) and IPMB cells (p = 0.003).
Pancreatic carcinomas (both IDC and IPMC) expressed significantly higher levels of LMO4 mRNA than did normal ductal epithelia, PanIN-1B, PanIN-2, IPMA and IPMB. These results suggested that LMO4 is overexpressed at late stages in carcinogenesis of pancreatic cancer.
Pancreatic cancer is one of the most aggressive malignant tumors. It is the fifth leading cause of cancer death in Japan [1, 2] and has the lowest survival rate of any solid cancer . Because the lack of specific symptoms in patients with pancreatic cancer makes early diagnosis difficult, initial diagnosis typically occurs when the tumor has reached an advanced stage . A better understanding of pancreatic carcinogenesis is urgently needed to facilitate early detection. Pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary-mucinous neoplasm (IPMN) were reported to be precursor lesions of pancreatic cancer [5–8]. Development of invasive ductal adenocarcinoma has been proposed to occur via two pathways [9–11], the PanIN-Invasive ductal carcinoma (IDC) progression pathway and the IPM adenoma (IPMA)-invasive IPM carcinoma (IPMC) pathway, although some specific subtypes of IPMN, such as intestinal-type IPMN, may not progress to invasive carcinoma through the same genetic pathway as PanIN. Longnecker et al  reported that PanIN-1 and IPMA showed mild dysplasia (grade 1), PanIN-2 and IPM borderline (IPMB) lesions showed moderate dysplasia (grade 2), and PanIN-3 and IPMC (carcinoma in situ [CIS]) showed severe dysplasia (grade 3).
LIM-only 4 (LMO4) is one of the four members (LMOs 1, 2, 3 and 4) of the LIM-only subfamily of LIM domain proteins. LIM domains are an approximately 55-amino acid, cysteine-rich, zinc-binding motif that mediate protein-protein interactions present in a variety of proteins including LIM homeobox proteins . The nuclear LIM-only proteins (LMOs 1–4) lack a DNA-binding domain but still function as transcriptional regulators by recruiting other protein partners including transcription factors [14, 15]. Kenny et al  reported the isolation and characterization of LMO4, a novel LIM-only gene that is highly expressed in the T-lymphocyte lineage, cranial neural crest cells, somites, dorsal limb bud mesenchyme, motor neurons and Schwann cell progenitors. As well as its role in development, there are several lines of evidence suggesting that LMO4 may have roles in oncogenesis . LMO4, initially described as a human breast tumor autoantigen , was reported to have a role in maintaining proliferation of mammary epithelium and suggested that deregulation of this gene may contribute to breast tumorigenesis . Additionally, Sum et al  found that LMO4 interacts with the cofactor CtIP and the tumor suppressor breast cancer 1 (BRCA1), and inhibits the transcriptional activity of BRCA1 in both yeast and mammalian cells by functional assays. They concluded that deregulation of LMO4 in breast epithelium directly contributes to breast neoplasia by altering the rate of cellular proliferation and promoting cell invasion. In 2005, Sum and colleagues reported that LMO4 mRNA was overexpressed in 5 of 10 human breast cancer cell lines; in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of the specimens . It has also been reported that expression of LMO4 is up-regulated at the invasive front of oral cancer, suggesting a role in cancer cell invasion . It was recently reported that the bone morphogenic protein (BMP7) gene, which controls cell proliferation and apoptosis of mammary epithelial cells, is a direct target of LMO4 . Both pancreatic cancer and breast cancer are known to have an epithelial origin while pancreatic cancer also reveals papillo-tubular structures that are similar to the histological characteristics of the initial breast cancer . As well, known genetic changes in pancreatic cancer often involve the same genes as those found in breast cancer . Taken together, these findings suggest that LMO4 has critical functions in pancreatic carcinogenesis as well as in normal development. Thus clarification of the role of LMO4 may be useful for diagnosis and/or treatment of pancreatic cancer. However, little is known about the role of LMO4 in pancreatic cancer and carcinogenesis.
To determine whether LMO4 is correlated with pancreatic cancer and carcinogenesis, we quantified LMO4 mRNA levels in cultured pancreatic cell lines, bulk tissues and microdissection-based target cells (including normal pancreatic ductal, PanIN-1B and PanIN-2, IDC, IPMA, IPMB and IPMC cells), by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Our goal was to characterize LMO4 expression in the early and late stages of pancreatic carcinogenesis to clarify both if and when overexpression of LMO4 occurs.
Materials and methods
Fourteen pancreatic cancer cell lines, AsPC-1, KP-1N, KP-2, KP-3, PANC-1, SUIT-2 (provided by Dr. H. Iguchi, National Shikoku Cancer Center, Matsuyama, Japan), MIA PaCa-2 (Japanese Cancer Resource Bank, Tokyo, Japan), NOR-P1 (established in our laboratory), CAPAN-1, CAPAN-2, CFPAC-1, H48N, HS766T and SW1990 (American Type Culture Collection, Manassas, VA, USA), the HPDE cell line and six primary cultures of fibroblasts derived from pancreatic tumors were studied. Cells were maintained as described previously .
Total RNA was extracted from cultured cells with a High Pure RNA Isolation Kit (Roche, Mannheim, Germany). Total RNA was extracted from bulk tissues with an RNeasy Mini Kit (Qiagen, Tokyo, Japan) following the manufacturer's protocol. Total RNA was extracted from cells isolated by microdissection with the standard acid guanidinium thiocyanate-phenol-chloroform protocol  with or without glycogen (Funakoshi, Tokyo, Japan) .
Quantitative assessment of LMO4 level by real-time RT-PCR
Quantitative real-time RT-PCR was performed with a QuantiTect SYBR Green RT-PCR Kit (Qiagen, Tokyo, Japan) with a Chromo4™ System (Bio-Rad, Hercules, CA, USA). In brief, the reaction mixture was first incubated at 50°C for 30 min to allow for reverse transcription. PCR was initiated with one cycle of 95°C for 15 min to activate the modified Taq polymerase followed by 40 cycles of 94°C for 15 sec, 55°C for 20 sec, and 72°C for 10 sec, and one cycle of 95°C for 0 sec, 65°C for 15 sec and +0.1°C/sec to 95°C for melting analysis. Each sample was run in triplicate. The level of LMO4 mRNA expression was calculated from a standard curve constructed with total RNA from the SUIT-2 pancreatic cancer cell line. The range of threshold cycles was from 20–35 cycles for LMO4 primers (forward, 5'-GGA CCG CTT TCT GCT CTA TG-3'; reverse, 5'-AAG GAT CAT GCC ACT TTT GG-3'), and from 7–35 cycles for 18S rRNA primers (forward 5'-GAT ATG CTC ATG TGG TGT TG-3'; reverse, 5'-AAT CTT CTT CAG TCG CTC CA-3'). Expressions of LMO4 mRNA were normalized to that of 18S rRNA.
Microdissection-based quantitative analysis of LMO4 mRNA
Data were analyzed with multiple comparison in ANOVA (analysis of variance) and Bivariate Correlations with Statistics Package for Social Science (SPSS) software (SPSS Inc. Chicago, IL, USA) after Kolmogorov-Smirnov test to assure that each data set is showed a normal distribution. For multiple comparisons by ANOVA, we used the least significant difference (LSD) test and set the statistical significance at p < 0.05. We used Spearman test for bivariate correlations.
Quantitative analysis of LMO4 expression in 14 pancreatic cancer cell lines, a non-neoplastic ductal epithelial cell line and six primary cultures of pancreatic fibroblasts
Quantitative analyses of LMO4 expression in bulk pancreatic tissues
Microdissection-based quantitative analysis of LMO4 expression in IDC, PanIN-2, PanIN-1B and normal ductal cells
In general, bulk pancreatic tissues are composed of a various types of cells, including ductal epithelial, acinar, islet and mesenchymal cells, and fibroblasts. Cancer cells comprise only 30% – 70% of the cells in bulk tissue specimens of pancreatic cancer . Premalignant cells, such as PanINs, and normal ductal cells comprise even smaller percentages of the cells in non-malignant tissues. The results of our present analyses of cultured cells suggested that LMO4 was expressed in pancreatic fibroblasts (Figure 3). Therefore, to avoid the influence of contaminating non-ductal cells, we used a laser-microdissection (LMD) method to select specific ductal cells for further analysis.
Quantitative analyses of LMO4 expressions in IPMC, IPMB, IPMA and normal ductal cells
Quantitative analyses of LKB1 expressions in cultured and microdissected cells, and correlation analyses between LKB1 and LMO4 in pancreatic carcinogenesis
In the present study, we performed quantitative real-time RT-PCR to measure LMO4 expression during pancreatic carcinogenesis through the PanIN-IDC and IPMA-IPMC pathways. Analyses of cultured cells revealed that 9 of 14 pancreatic cancer cell lines and all primary cultures of pancreatic fibroblasts expressed higher levels of LMO4 than did a non-neoplastic pancreatic ductal cell line. Bulk tissue analysis showed that pancreatic cancer tissues expressed higher levels of LMO4 than non-neoplastic and non-malignant IPMN tissues; however, the difference in LMO4 expression between non-neoplastic tissues and non-malignant IPMN was not significant. To avoid the influence of LMO4-expressing non-ductal cells contained in bulk tissues, we microdissected target cells, such as IDCs, PanINs, IPMNs and non-neoplastic ductal cells, and measured LMO4 expression in the microdissected cells. It is usually difficult to obtain frozen sections of intermediate or high-grade PanIN-2 or PanIN-3 (CIS) or non-invasive IPMC (CIS) lesions. In the present study, we obtained frozen sections from 3 cases of PanIN-2 lesions and 3 of non-invasive IPMC. We found that the LMO4 expression in IDC cells was significantly higher than those in PanIN-1B, PanIN-2, and normal ductal cells; however, the PanIN-2 sample number was small. We also found that both invasive and non-invasive IPMC cells expressed higher levels of LMO4 than did non-malignant IPMN or normal ductal cells. By contrast, we could not detect any differences in LMO4 expression among PanIN-1B, PanIN-2 and normal ductal cells, or among IPMA, IPMB, and normal ductal cells. Taken together, these data suggested that LMO4 expression is up-regulated in pancreatic cancer but not in low-grade intraductal precursors in both the PanIN-IDC and IPMA-IPMC pathways.
This is the first report to use qRT-PCR for analyses of LMO4 expression during pancreatic carcinogenesis. LMO4 is reported to have an oncogenic role in carcinogenesis and in carcinoma progression in breast cancer and SCC [18–21]. However, the human LMO4 gene is located on chromosome 1p22.3 , which is a region deleted in several human cancers, such as those of liver, skin, and lung [36, 37], and Setogawa et al reported that the tumor suppressor LKB1 induces p21 expression in collaboration with LMO4, suggesting that LMO4 may have a tumor suppressor function . In the present study, we found that there was significant correlation between LMO4 and LKB1 in both primary cultured fibroblasts and microdissected non-malignant cells, but there was not a significant correlation between LMO4 and LKB1 in cancer cells. We also found the downregulation of LKB1 mRNA in IDC cells, consistent with LKB1's tumor suppressive function. Our data suggest that alteration of the LKB1-LMO4 balance is involved in pancreatic carcinogenesis, although the exact function of LMO4 in pancreatic carcinogenesis remains unknown. Taken together, there appears to be a conflicting function of LMO4 in carcinogenesis as a tumor suppressor or as an oncogene; it is reasonable that LMO4, a transcription regulator, may have multiple functions in individual cancers, like E2F1, an another transcription factor, that was reported both as an oncogene by stimulating cell proliferation  and as a tumor suppressor by signaling p53-dependent apoptosis .
Recently, Murphy et al  used immunohistochemical staining and reported that a subset of patients with low LMO4 expression-pancreatic cancers had poor outcomes. In the present study, LMO4 mRNA was not overexpressed in any of the 14 pancreatic cancer cell lines. However, all IDC cells microdissected from cancer tissues showed relatively high expression of LMO4 mRNA although the sample number was small. This might have been the result of using of frozen sections with a histological diagnosis of moderately or well-differentiated adenocarcinoma, which can be microdissected easily. Patients with well-differentiated adenocarcinoma usually have better prognosis ; thus the present data may be partially consistent with Murphy's result demonstrating that high LMO4-pancreatic cancers are associated with a significant survival advantage for patients with surgical resection.
Taken together, it remains unclear if LMO4 has an oncogenic function or a tumor suppressive function in pancreatic carcinogenesis. To better clarify the functional roles of LMO4 in pancreatic carcinogenesis, further examinations such as inhibition experiments using RNAi technology are needed.
In conclusion, the present results showed that LMO4 is overexpressed in pancreatic cancer related to both the PanIN-conventional IDC pathway and the IPMA-IPMC pathway, but not at the early stages of pancreatic carcinogenesis.
We thank Miyuki Ohmori for expert technical assistance in preparing frozen sections for microdissection, and thank Shoko Sadatomi, Midori Sato, and Emiko Manabe for help in maintaining the cultures of cell lines and clinical samples. This work has been supported by the grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan; the Fukuoka Cancer Society, Pancreas Research Foundation of Japan; Clinical Research Foundation; and Kaibara Morikazu Medical Science Promotion Foundation. We appreciate for the technical supports from the Research Support Center, Graduate School of Medical Sciences, Kyushu University, Japan.
- Warshaw AL, Fernandez-del Castillo C: Pancreatic carcinoma. N Engl J Med. 1992, 326: 455-65.View ArticlePubMedGoogle Scholar
- Yamamoto M, Ohashi O, Saitoh Y: Japan Pancreatic Cancer Registry: current status. Pancreas. 1998, 16: 238-42. 10.1097/00006676-199804000-00006View ArticlePubMedGoogle Scholar
- Matsuno S, Egawa S, Fukuyama S, Motoi F, Sunamura M, Isaji S: Pancreatic Cancer Registry in Japan: 20 years of experience. Pancreas. 2004, 28: 219-30. 10.1097/00006676-200404000-00002View ArticlePubMedGoogle Scholar
- Tanaka M: Important clues to the diagnosis of pancreatic cancer. Roczniki Akademii Medycznej w Bialymstoku (1995). 2005, 50: 69-72.Google Scholar
- House MG, Guo M, Iacobuzio-Donahue C, Herman JG: Molecular progression of promoter methylation in intraductal papillary mucinous neoplasms (IPMN) of the pancreas. Carcinogenesis. 2003, 24: 193-8. 10.1093/carcin/24.2.193View ArticlePubMedGoogle Scholar
- Hruban RH, Takaori K, Klimstra DS, Adsay NV, Albores-Saavedra J, Biankin AV: An illustrated consensus on the classification of pancreatic intraepithelial neoplasia and intraductal papillary mucinous neoplasms. Am J Surg Pathol. 2004, 28: 977-87. 10.1097/01.pas.0000126675.59108.80View ArticlePubMedGoogle Scholar
- Klimstra DS, Longnecker DS: K-ras mutations in pancreatic ductal proliferative lesions. The American journal of pathology. 1994, 145: 1547-50.PubMed CentralPubMedGoogle Scholar
- Nakata B, Yashiro M, Nishioka N, Aya M, Yamada S, Takenaka C: Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas. Int J Oncol. 2002, 21: 1067-72.PubMedGoogle Scholar
- Adsay NV, Conlon KC, Zee SY, Brennan MF, Klimstra DS: Intraductal papillary-mucinous neoplasms of the pancreas: an analysis of in situ and invasive carcinomas in 28 patients. Cancer. 2002, 94: 62-77. 10.1002/cncr.10203View ArticlePubMedGoogle Scholar
- Hruban RH, Adsay NV, Albores-Saavedra J, Compton C, Garrett ES, Goodman SN: Pancreatic intraepithelial neoplasia: a new nomenclature and classification system for pancreatic duct lesions. Am J Surg Pathol. 2001, 25: 579-86. 10.1097/00000478-200105000-00003View ArticlePubMedGoogle Scholar
- Takaori K, Kobashi Y, Matsusue S, Matsui K, Yamamoto T: Clinicopathological features of pancreatic intraepithelial neoplasias and their relationship to intraductal papillary-mucinous tumors. J Hepatobiliary Pancreat Surg. 2003, 10: 125-36. 10.1007/s00534-003-0756-8View ArticlePubMedGoogle Scholar
- Longnecker DS, Adsay NV, Fernandez-del Castillo C, Hruban RH, Kasugai T, Klimstra DS: Histopathological diagnosis of pancreatic intraepithelial neoplasia and intraductal papillary-mucinous neoplasms: interobserver agreement. Pancreas. 2005, 31: 344-9. 10.1097/01.mpa.0000186245.35716.18View ArticlePubMedGoogle Scholar
- Kenny DA, Jurata LW, Saga Y, Gill GN: Identification and characterization of LMO4, an LMO gene with a novel pattern of expression during embryogenesis. Proc Natl Acad Sci USA. 1998, 95: 11257-62. 10.1073/pnas.95.19.11257PubMed CentralView ArticlePubMedGoogle Scholar
- Boehm T, Foroni L, Kaneko Y, Perutz MF, Rabbitts TH: The rhombotin family of cysteine-rich LIM-domain oncogenes: distinct members are involved in T-cell translocations to human chromosomes 11p15 and 11p13. Proc Natl Acad Sci USA. 1991, 88: 4367-71. 10.1073/pnas.88.10.4367PubMed CentralView ArticlePubMedGoogle Scholar
- Royer-Pokora B, Loos U, Ludwig WD: TTG-2, a new gene encoding a cysteine-rich protein with the LIM motif, is overexpressed in acute T-cell leukaemia with the t(11;14)(p13;q11). Oncogene. 1991, 6: 1887-93.PubMedGoogle Scholar
- Lu Z, Lam KS, Wang N, Xu X, Cortes M, Andersen B: LMO4 can interact with Smad proteins and modulate transforming growth factor-beta signaling in epithelial cells. Oncogene. 2006, 25: 2920-30. 10.1038/sj.onc.1209318View ArticlePubMedGoogle Scholar
- Racevskis J, Dill A, Sparano JA, Ruan H: Molecular cloning of LMO41, a new human LIM domain gene. Biochim Biophys Acta. 1999, 1445: 148-53.View ArticlePubMedGoogle Scholar
- Visvader JE, Venter D, Hahm K, Santamaria M, Sum EY, O'Reilly L: The LIM domain gene LMO4 inhibits differentiation of mammary epithelial cells in vitro and is overexpressed in breast cancer. Proc Natl Acad Sci USA. 2001, 98: 14452-7. 10.1073/pnas.251547698PubMed CentralView ArticlePubMedGoogle Scholar
- Sum EY, Peng B, Yu X, Chen J, Byrne J, Lindeman GJ: The LIM domain protein LMO4 interacts with the cofactor CtIP and the tumor suppressor BRCA1 and inhibits BRCA1 activity. J Biol Chem. 2002, 277: 7849-56. 10.1074/jbc.M110603200View ArticlePubMedGoogle Scholar
- Sum EY, Segara D, Duscio B, Bath ML, Field AS, Sutherland RL: Overexpression of LMO4 induces mammary hyperplasia, promotes cell invasion, and is a predictor of poor outcome in breast cancer. Proc Natl Acad Sci USA. 2005, 102: 7659-64. 10.1073/pnas.0502990102PubMed CentralView ArticlePubMedGoogle Scholar
- Mizunuma H, Miyazawa J, Sanada K, Imai K: The LIM-only protein, LMO4, and the LIM domain-binding protein, LDB1, expression in squamous cell carcinomas of the oral cavity. British journal of cancer. 2003, 88: 1543-8. 10.1038/sj.bjc.6600952PubMed CentralView ArticlePubMedGoogle Scholar
- Wang N, Lin KK, Lu Z, Lam KS, Newton R, Xu X: The LIM-only factor LMO4 regulates expression of the BMP7 gene through an HDAC2-dependent mechanism, and controls cell proliferation and apoptosis of mammary epithelial cells. Oncogene. 2007, 26: 6431-41. 10.1038/sj.onc.1210465View ArticlePubMedGoogle Scholar
- Kitamura N, Murata S, Abe H, Hanasawa K, Tsukashita S, Tani T: Obstructive jaundice in a metastatic tumor of the pancreas from breast cancer: a case report. Japanese Journal of clinical oncology. 2003, 33: 93-97. 10.1093/jjco/hyg018View ArticlePubMedGoogle Scholar
- Schutte M, Hruban RH, Hedrick L, Cho KR, Nadasdy GM, Weinstein CL: DPC4 gene in various tumor types. Cancer research. 1996, 56: 2527-30.PubMedGoogle Scholar
- Ohuchida K, Mizumoto K, Murakami M, Qian LW, Sato N, Nagai E: Radiation to stromal fibroblasts increases invasiveness of pancreatic cancer cells through tumor-stromal interactions. Cancer research. 2004, 64: 3215-22. 10.1158/0008-5472.CAN-03-2464View ArticlePubMedGoogle Scholar
- Yu J, Ohuchida K, Mizumoto K, Ishikawa N, Ogura Y, Yamada D: Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer. World J Gastroenterol. 2006, 12: 3878-82.PubMed CentralPubMedGoogle Scholar
- Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical biochemistry. 1987, 162: 156-9. 10.1016/0003-2697(87)90021-2View ArticlePubMedGoogle Scholar
- Tachikawa T, Irie T: A new molecular biology approach in morphology: basic method and application of laser microdissection. Med Electron Microsc. 2004, 37: 82-8.PubMedGoogle Scholar
- Liu N, Furukawa T, Kobari M, Tsao MS: Comparative phenotypic studies of duct epithelial cell lines derived from normal human pancreas and pancreatic carcinoma. The American journal of pathology. 1998, 153: 263-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Ouyang H, Mou L, Luk C, Liu N, Karaskova J, Squire J: Immortal human pancreatic duct epithelial cell lines with near normal genotype and phenotype. The American journal of pathology. 2000, 157: 1623-31.PubMed CentralView ArticlePubMedGoogle Scholar
- Jenne DE, Reimann H, Nezu J, Friedel W, Loff S, Jeschke R: Peutz-Jeghers syndrome is caused by mutations in a novel serine threonine kinase. Nature genetics. 1998, 18: 38-43. 10.1038/ng0198-38View ArticlePubMedGoogle Scholar
- Sato N, Rosty C, Jansen M, Fukushima N, Ueki T, Yeo CJ: STK11/LKB1 Peutz-Jeghers gene inactivation in intraductal papillary-mucinous neoplasms of the pancreas. The American journal of pathology. 2001, 159: 2017-22.PubMed CentralView ArticlePubMedGoogle Scholar
- Su GH, Hruban RH, Bansal RK, Bova GS, Tang DJ, Shekher MC: Germline and somatic mutations of the STK11/LKB1 Peutz-Jeghers gene in pancreatic and biliary cancers. The American journal of pathology. 1999, 154: 1835-40.PubMed CentralView ArticlePubMedGoogle Scholar
- Setogawa T, Shinozaki-Yabana S, Masuda T, Matsuura K, Akiyama T: The tumor suppressor LKB1 induces p21 expression in collaboration with LMO4, GATA-6, and Ldb1. Biochemical and biophysical research communications. 2006, 343: 1186-90. 10.1016/j.bbrc.2006.03.077.View ArticlePubMedGoogle Scholar
- Tse E, Grutz G, Garner AA, Ramsey Y, Carter NP, Copeland N: Characterization of the Lmo4 gene encoding a LIM-only protein: genomic organization and comparative chromosomal mapping. Mamm Genome. 1999, 10: 1089-94. 10.1007/s003359901167View ArticlePubMedGoogle Scholar
- Emi M, Matsumoto S, Iida A, Tsukamoto K, Nakata T, Yokota T: Correlation of Allelic Losses and Clinicopathological Factors in Primary Breast Cancers. Breast Cancer. 1997, 4: 243-6. 10.1007/BF02966514View ArticlePubMedGoogle Scholar
- Hoggard N, Brintnell B, Howell A, Weissenbach J, Varley J: Allelic imbalance on chromosome 1 in human breast cancer. II. Microsatellite repeat analysis. Genes, chromosomes & cancer. 1995, 12: 24-31. 10.1002/gcc.2870120105View ArticleGoogle Scholar
- Singh P, Wong SH, Hong W: Overexpression of E2F-1 in rat embryo fibroblasts leads to neoplastic transformation. The EMBO journal. 1994, 13: 3329-38.PubMed CentralPubMedGoogle Scholar
- Jamshidi-Parsian A, Dong Y, Zheng X, Zhou HS, Zacharias W, McMasters KM: Gene expression profiling of E2F-1-induced apoptosis. Gene. 2005, 344: 67-77. 10.1016/j.gene.2004.09.030View ArticlePubMedGoogle Scholar
- Murphy NC, Scarlett CJ, Kench JG, Sum EYM, Segara D, Colvin EK: Expression of LMO4 and outcome in pancreatic ductal adenocarcinoma. British Journal of Cancer. 2008, 98: 537-41. 10.1038/sj.bjc.6604177PubMed CentralView ArticlePubMedGoogle Scholar
- Pongprasobchai S, Pannala R, Smyrk TC, Bamlet W, Pitchumoni S, Ougolkov A: Long-term survival and prognostic indicators in small (< or = 2 cm) pancreatic cancer. Pancreatology. 2008, 8: 587-92. 10.1159/000161009PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.